公开(公告)号：US20160215281A1

公开(公告)日：2016-07-28

申请号：US15003179

申请日：2016-01-21

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Song-Hua KE ,  Craig BRANCH ,  Jun LEE ,  Todd Peterson ,  Karl HECKER

IPC: C12N15/10 ,  C12Q1/68

CPC classification number: C12N15/1003 ,  C07H1/00 ,  C12Q1/6806 ,  C12Q2527/125 ,  C12Q2527/101

Abstract: Disclosed are rapid and reproducible methods for separating and purifying nucleic acids from paraffin-containing tissue samples. The disclosed methods involve a melting step, where paraffin-containing tissue samples are heated in the presence of a detergent, causing the paraffin to melt and tissue sample cells to lyse. From the resulting two-phase mixture (i.e., a paraffin phase and an aqueous phase), the aqueous phase is collected for purification of nucleic acid. Protease(s) can be used at one or more points in the separation process to facilitate tissue cell lysis and/or degrade proteins that could degrade nucleic acid or interfere with subsequent genetic manipulation or analysis.

Abstract translation: 公开了用于从含石蜡组织样品中分离和纯化核酸的快速和可重复的方法。 所公开的方法包括熔融步骤，其中在洗涤剂存在下加热含石蜡的组织样品，使石蜡熔化并组织样品细胞裂解。 从所得的两相混合物（即，石蜡相和水相）中收集水相以纯化核酸。 可以在分离过程中的一个或多个点使用蛋白酶，以促进组织细胞裂解和/或降解可能降解核酸或干扰后续遗传操作或分析的蛋白质。