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公开(公告)号：US11046953B2
公开(公告)日：2021-06-29
申请号：US16678576
申请日：2019-11-08
Inventor： Todd Peterson , Axel Trefzer , Thomas Poehmerer
Abstract: The disclosure generally relates to compositions and methods for the production of nucleic acid molecules. In some aspects, the invention allows for the microscale generation of nucleic acid molecules, optionally followed by assembly of these nucleic acid molecules into larger molecules. In some aspects, the invention allows for efficient production of nucleic acid molecules (e.g., large nucleic acid molecules such as genomes).
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2.发明申请SEPARATION AND PURIFICATION OF NUCLEIC ACID FROM PARAFFIN-CONTAINING SAMPLES 审中-公开从含PARAFFIN的样品中分离和纯化核酸公开(公告)号：US20160215281A1
公开(公告)日：2016-07-28
申请号：US15003179
申请日：2016-01-21
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Song-Hua KE , Craig BRANCH , Jun LEE , Todd Peterson , Karl HECKER
CPC classification number: C12N15/1003 , C07H1/00 , C12Q1/6806 , C12Q2527/125 , C12Q2527/101
Abstract: Disclosed are rapid and reproducible methods for separating and purifying nucleic acids from paraffin-containing tissue samples. The disclosed methods involve a melting step, where paraffin-containing tissue samples are heated in the presence of a detergent, causing the paraffin to melt and tissue sample cells to lyse. From the resulting two-phase mixture (i.e., a paraffin phase and an aqueous phase), the aqueous phase is collected for purification of nucleic acid. Protease(s) can be used at one or more points in the separation process to facilitate tissue cell lysis and/or degrade proteins that could degrade nucleic acid or interfere with subsequent genetic manipulation or analysis.
Abstract translation: 公开了用于从含石蜡组织样品中分离和纯化核酸的快速和可重复的方法。 所公开的方法包括熔融步骤，其中在洗涤剂存在下加热含石蜡的组织样品，使石蜡熔化并组织样品细胞裂解。 从所得的两相混合物（即，石蜡相和水相）中收集水相以纯化核酸。 可以在分离过程中的一个或多个点使用蛋白酶，以促进组织细胞裂解和/或降解可能降解核酸或干扰后续遗传操作或分析的蛋白质。
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公开(公告)号：US20150044758A1
公开(公告)日：2015-02-12
申请号：US14462640
申请日：2014-08-19
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Joseph Amshey , Daniel Bezdek , Espir Kahatt , Alexander Khorlin , Lance Parsons , Todd Peterson , Timothy Powers , Michael Thacker , Timothy Updyke , Kornelija Zgonc
IPC: B01L3/00
CPC classification number: B01L3/502715 , B01L3/50273 , B01L7/00 , B01L9/527 , B01L2200/025 , B01L2200/027 , B01L2200/0689 , B01L2200/10 , B01L2200/16 , B01L2300/0636 , B01L2300/0681 , B01L2300/0867 , B01L2300/088 , B01L2300/123 , B01L2400/0481 , B01L2400/0487 , B01L2400/0605 , B01L2400/0655 , G01N2035/00306 , G01N2035/1037 , Y10T436/2575
Abstract: The present invention provides systems, devices, apparatuses and methods for automated bioprocessing. Examples of protocols and bioprocessing procedures suitable for the present invention include but are not limited to immunoprecipitation, chromatin immunoprecipitation, recombinant protein isolation, nucleic acid separation and isolation, protein labeling, separation and isolation, cell separation and isolation, food safety analysis and automatic bead based separation. In some embodiments, the invention provides automated systems, automated devices, automated cartridges and automated methods of western blot processing. Other embodiments include automated systems, automated devices, automated cartridges and automated methods for separation, preparation and purification of nucleic acids, such as DNA or RNA or fragments thereof, including plasmid DNA, genomic DNA, bacterial DNA, viral DNA and any other DNA, and for automated systems, automated devices, automated cartridges and automated methods for processing, separation and purification of proteins, peptides and the like.
Abstract translation: 本发明提供了用于自动化生物处理的系统，装置，装置和方法。 适用于本发明的方案和生物处理方法的实例包括但不限于免疫沉淀，染色质免疫沉淀，重组蛋白分离，核酸分离和分离，蛋白质标记，分离和分离，细胞分离和分离，食品安全分析和自动珠 基于分离。 在一些实施方案中，本发明提供自动化系统，自动化装置，自动化盒和western印迹处理的自动化方法。 其他实施方案包括自动化系统，自动化装置，自动化盒和用于分离，制备和纯化核酸例如DNA或RNA或其片段的自动化方法，包括质粒DNA，基因组DNA，细菌DNA，病毒DNA和任何其它DNA， 以及用于自动化系统，自动化设备，自动化盒和用于处理，分离和纯化蛋白质，肽等的自动化盒和自动化方法。
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公开(公告)号：US11235323B2
公开(公告)日：2022-02-01
申请号：US16557192
申请日：2019-08-30
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Joseph Amshey , Daniel Bezdek , Espir Kahatt , Alexander Khorlin , Lance Parsons , Todd Peterson , Timothy Powers , Michael Thacker , Timothy Updyke , Kornelija Zgonc
Abstract: The present invention provides systems, devices, apparatuses and methods for automated bioprocessing. Examples of protocols and bioprocessing procedures suitable for the present invention include but are not limited to: immunoprecipitation, chromatin immunoprecipitation, recombinant protein isolation, nucleic acid separation and isolation, protein labeling, separation and isolation, cell separation and isolation, food safety analysis and automatic bead based separation. In some embodiments, the invention provides automated systems, automated devices, automated cartridges and automated methods of western blot processing. Other embodiments include automated systems, automated devices, automated cartridges and automated methods for separation, preparation and purification of nucleic acids, such as DNA or RNA or fragments thereof, including plasmid DNA, genomic DNA, bacterial DNA, viral DNA and any other DNA, and for automated systems, automated devices, automated cartridges and automated methods for processing, separation and purification of proteins, peptides and the like.
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公开(公告)号：US10280428B2
公开(公告)日：2019-05-07
申请号：US15840273
申请日：2017-12-13
Applicant: Life Technologies Corporation
Inventor： Farzad Haerizadeh , Todd Peterson , Wen Chen , Ewa Lis
Abstract: The present invention provides compositions and methods for the genetic manipulation of Algal cells. The compositions and methods allow enhanced transfer of genetic material into Algal cells and the cloning and selection of genetically modified cells. Expression of proteins encoded by the genetic material will be enhanced by the methods and compositions of the invention.
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Patent Agency Ranking
公开(公告)号：US12209239B2
公开(公告)日：2025-01-28
申请号：US17320572
申请日：2021-05-14
Inventor： Todd Peterson , Axel Trefzer , Thomas Poehmerer
Abstract: The disclosure generally relates to compositions and methods for the production of nucleic acid molecules. In some aspects, the invention allows for the microscale generation of nucleic acid molecules, optionally followed by assembly of these nucleic acid molecules into larger molecules. In some aspects, the invention allows for efficient production of nucleic acid molecules (e.g., large nucleic acid molecules such as genomes).
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公开(公告)号：US10434509B2
公开(公告)日：2019-10-08
申请号：US15719130
申请日：2017-09-28
Applicant: Life Technologies Corporation
Inventor： Joseph Amshey , Daniel Bezdek , Espir Kahatt , Alexander Khorlin , Lance Parsons , Todd Peterson , Timothy Powers , Michael Thacker , Timothy Updyke , Kornelija Zgonc
Abstract: The present invention provides systems, devices, apparatuses and methods for automated bioprocessing. Examples of protocols and bioprocessing procedures suitable for the present invention include but are not limited to: immunoprecipitation, chromatin immunoprecipitation, recombinant protein isolation, nucleic acid separation and isolation, protein labeling, separation and isolation, cell separation and isolation, food safety analysis and automatic bead based separation. In some embodiments, the invention provides automated systems, automated devices, automated cartridges and automated methods of western blot processing. Other embodiments include automated systems, automated devices, automated cartridges and automated methods for separation, preparation and purification of nucleic acids, such as DNA or RNA or fragments thereof, including plasmid DNA, genomic DNA, bacterial DNA, viral DNA and any other DNA, and for automated systems, automated devices, automated cartridges and automated methods for processing, separation and purification of proteins, peptides and the like.
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公开(公告)号：US20180179548A1
公开(公告)日：2018-06-28
申请号：US15840273
申请日：2017-12-13
Applicant: Life Technologies Corporation
Inventor： Farzad HAERIZADEH , Todd Peterson , Wen Chen , Ewa Lis
CPC classification number: C12N15/8206 , C12N1/12 , C12N5/0025 , C12N15/79 , C12N15/87
Abstract: The present invention provides compositions and methods for the genetic manipulation of Algal cells. The compositions and methods allow enhanced transfer of genetic material into Algal cells and the cloning and selection of genetically modified cells. Expression of proteins encoded by the genetic material will be enhanced by the methods and compositions of the invention.
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公开(公告)号：US10563240B2
公开(公告)日：2020-02-18
申请号：US14775648
申请日：2014-03-14
Inventor： Todd Peterson , Axel Trefzer , Thomas Poehmerer , Federico Katzen , Xiquan Liang
IPC: C12P19/34
Abstract: The disclosure generally relates to compositions and methods for the production of nucleic acid molecules. In some aspects, the invention allows for the microscale generation of nucleic acid molecules, optionally followed by assembly of these nucleic acid molecules into larger molecules. In some aspects, the invention allows for efficient production of nucleic acid molecules (e.g., large nucleic acid molecules such as genomes).
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公开(公告)号：US10519439B2
公开(公告)日：2019-12-31
申请号：US15463363
申请日：2017-03-20
Inventor： Todd Peterson , Axel Trefzer , Thomas Poehmerer
Abstract: The disclosure generally relates to compositions and methods for the production of nucleic acid molecules. In some aspects, the invention allows for the microscale generation of nucleic acid molecules, optionally followed by assembly of these nucleic acid molecules into larger molecules. In some aspects, the invention allows for efficient production of nucleic acid molecules (e.g., large nucleic acid molecules such as genomes).
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