公开(公告)号：US20140272940A1

公开(公告)日：2014-09-18

申请号：US14206716

申请日：2014-03-12

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Wayne Ge ,  Craig Cummings ,  Darcy Myers ,  Quoc Hoang ,  Catherine O'Connell ,  Maxim Brevnov ,  Daniel Kephart

IPC: C12Q1/68

CPC classification number: C12Q1/686 ,  C12Q1/6809 ,  C12Q2525/155 ,  C12Q2525/161 ,  C12Q2525/197 ,  C12Q2531/113

Abstract: The present disclosure, in some embodiments, relates to compositions and methods for detection of multiple target nucleic acid sequences by a single nucleic acid amplification based assay (e.g. PCR). Compositions comprising two or more primers are described for multiple target nucleic acid detection. Methods for differential detection of microorganisms (including strains/serovars/subtypes thereof) and cell types comprise a single step method to detect a signature of target nucleic acid sequences comprising: two, three or more target nucleic acids that are uniquely present in a microorganism/strain/serovar/subtype and/or cell and absent in other closely related organisms/cells. Embodiments relate to methods of diagnosis of diseases or conditions that can be detected by detecting the presence of two or more nucleic acid target markers.

Abstract translation: 在一些实施方案中，本公开涉及通过基于单个核酸扩增的测定（例如PCR）检测多个靶核酸序列的组合物和方法。 描述了包含两个或多个引物的组合物用于多靶核酸检测。 用于差异检测微生物（包括菌株/血清型/亚型）和细胞类型的方法包括检测靶核酸序列的特征的单步方法，其包括：两个或三个以上唯一存在于微生物/ 菌株/血清型/亚型和/或细胞，并且在其他紧密相关的生物/细胞中不存在。 实施方案涉及通过检测两种或多种核酸靶标志物的存在可以检测的疾病或病症的诊断方法。