公开(公告)号：US09804156B2

公开(公告)日：2017-10-31

申请号：US13787635

申请日：2013-03-06

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Niroshan Ramachandran ,  Lihao Meng ,  Gengxin Chen ,  David Bourdon ,  Suzy Van Le

IPC: A61K38/00 ,  G01N33/564 ,  G01N33/68

CPC classification number: G01N33/564 ,  G01N33/6893 ,  G01N2333/52 ,  G01N2333/523 ,  G01N2800/104 ,  G01N2800/52

Abstract: The disclosure provides SLE biomarkers. The disclosure further provides kits and methods of diagnosing, prognosing, and stratifying subjects with the disease by utilizing SLE biomarkers.

公开(公告)号：US10364414B2

公开(公告)日：2019-07-30

申请号：US15700474

申请日：2017-09-11

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Philip Martin ,  Paul Nelson ,  David Bourdon

IPC: C12N1/06 ,  C12M1/00 ,  C07K1/22 ,  C07K1/18 ,  C07K1/14 ,  C07K1/34

Abstract: A cell lysis workflow involving a cell suspension that is passed through a filter in a first flow direction from a first side of the filter toward a second side thereof. The filter captures a plurality of cells on the first side. A lysis solution is passed through the filter in a second flow direction opposite the first direction, thereby dislodging the plurality of cells captured on the filter, and resuspending the plurality of cells in the lysis solution on the first side of the filter. The lysis solution lyses the plurality of cells to produce a cellular lysate. The cellular lysate is passed through the filter in the first flow direction.