公开(公告)号：US09758755B2

公开(公告)日：2017-09-12

申请号：US14921741

申请日：2015-10-23

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Philip Martin

IPC: C12N1/06 ,  C12M1/00 ,  C07K1/22 ,  C07K1/18 ,  C07K1/16

CPC classification number: C12M47/06 ,  C07K1/145 ,  C07K1/18 ,  C07K1/22 ,  C07K1/34 ,  C12M29/04 ,  C12M29/16

Abstract: A cell lysis workflow involving a cell suspension that is passed through a filter in a first flow direction from a first side of the filter toward a second side thereof. The filter captures a plurality of cells on the first side. A lysis solution is passed through the filter in a second flow direction opposite the first direction, thereby dislodging the plurality of cells captured on the filter, and resuspending the plurality of cells in the lysis solution on the first side of the filter. The lysis solution lyses the plurality of cells to produce a cellular lysate. The cellular lysate is passed through the filter in the first flow direction.

公开(公告)号：US10364414B2

公开(公告)日：2019-07-30

申请号：US15700474

申请日：2017-09-11

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Philip Martin ,  Paul Nelson ,  David Bourdon

IPC: C12N1/06 ,  C12M1/00 ,  C07K1/22 ,  C07K1/18 ,  C07K1/14 ,  C07K1/34

Abstract: A cell lysis workflow involving a cell suspension that is passed through a filter in a first flow direction from a first side of the filter toward a second side thereof. The filter captures a plurality of cells on the first side. A lysis solution is passed through the filter in a second flow direction opposite the first direction, thereby dislodging the plurality of cells captured on the filter, and resuspending the plurality of cells in the lysis solution on the first side of the filter. The lysis solution lyses the plurality of cells to produce a cellular lysate. The cellular lysate is passed through the filter in the first flow direction.