公开(公告)号：US20190241942A1

公开(公告)日：2019-08-08

申请号：US16228304

申请日：2018-12-20

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Shiaw-Min CHEN ,  David W. RUFF ,  Harrison LEONG

IPC: C12Q1/6844 ,  G16B25/00 ,  G16B40/00

CPC classification number: C12Q1/6844 ,  G16B25/00 ,  G16B40/00 ,  C12Q2545/114

Abstract: Various embodiments of methods for analyzing proximity binding assay (PBA) data are disclosed. Proximity binding assays as a class of analyses offer the advantages of the sensitivity and specificity of biorecognition binding, along with the exponential signal amplification offered by a variety of oligonucleotide amplification reactions, such as the polymerase chain reaction (PCR). However, as various proximity binding assays have reaction kinetics governed by an additional step of the binding of a biorecognition probe (BRP) with a target molecule, there is a need for methods for the analysis of PBA data that are particularly suited to the unique characteristics of such data.