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    NUCLEIC ACID AMPLIFICATION
    1.
    发明申请
    NUCLEIC ACID AMPLIFICATION 审中-公开

    公开(公告)号：US20190338258A1

    公开(公告)日：2019-11-07

    申请号：US16442341

    申请日：2019-06-14

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Chieh-Yuan LI ,  David RUFF ,  Shiaw-Min CHEN ,  Jennifer O'NEIL ,  Rachel KASINSKAS ,  Jonathan ROTHBERG ,  Bin LI ,  Kai Qin LAO

    IPC: C12N9/12 ,  C12Q1/6844 ,  C12Q1/6853 ,  C12Q1/686 ,  C12Q1/6855

    Abstract: The present disclosure provides methods, compositions, kits and systems for nucleic acid amplification. In some embodiments, nucleic acid amplification methods include subjecting the nucleic acid to be amplified to partially denaturing conditions. In some embodiments, nucleic acid amplification methods include amplifying without fully denaturing the nucleic acid that is amplified. In some embodiments, the nucleic acid amplification method employs an enzyme that catalyzes homologous recombination and a polymerase. In some embodiments, methods for nucleic acid amplification can be conducted in a single reaction vessel and/or in a single continuous liquid phase of a reaction mixture, without need for compartmentalization of the reaction mixture or immobilization of reaction components. In some embodiments, methods for nucleic acid amplification comprise amplifying at least one polynucleotide onto a surface under isothermal amplification conditions, optionally in the presence of a polymer which can include a sieving agent and/or a diffusion-reducing agent.

    IMPROVED AMPLIFICATION AND SEQUENCING METHODS
    2.
    发明申请
    IMPROVED AMPLIFICATION AND SEQUENCING METHODS 审中-公开

    公开(公告)号：US20190048335A1

    公开(公告)日：2019-02-14

    申请号：US16085815

    申请日：2017-03-17

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Chieh-Yuan LI ,  David JOUN ,  Shiaw-Min CHEN

    IPC: C12N15/10 ,  C12Q1/6806 ,  C12N15/11 ,  C40B20/04

    Abstract: In some embodiments, the disclosure relates generally to methods, as well as related systems, compositions, kits, and apparatuses for any one or any combination of: conducting a library preparation method which generates a mixture of desirable template polynucleotides and non-desirable polynucleotide byproducts, amplifying the resulting library, enriching the desirable template polynucleotides, and sequencing the enriched template polynucleotides. The methods, as well as related systems, compositions, kits, and apparatuses, of the present teachings can be used to improve sequencing data.

    NUCLEIC ACID AMPLIFICATION
    3.
    发明申请
    NUCLEIC ACID AMPLIFICATION 有权

    公开(公告)号：US20140147852A1

    公开(公告)日：2014-05-29

    申请号：US14085727

    申请日：2013-11-20

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Chieh-Yuan LI ,  David RUFF ,  Jennifer O'NEIL ,  Rachel KASINSKAS ,  Shiaw-Min CHEN ,  Jonathan ROTHBERG

    IPC: C12Q1/68

    CPC classification number: C12Q1/6806 ,  C12Q1/6846 ,  C12Q1/6853 ,  C12Q1/686 ,  C12Q1/6874 ,  C12Q2531/119 ,  C12Q2565/537

    Abstract: In some embodiments, the present teachings provide methods for nucleic acid amplification, comprising forming a reaction mixture, and subjecting the reaction mixture to conditions suitable for nucleic acid amplification. In some embodiments, methods for nucleic acid amplification include subjecting the nucleic acid to be amplified to partially denaturing conditions. In some embodiments, methods for nucleic acid amplification include amplifying without fully denaturing the nucleic acid that is amplified. In some embodiments, the methods for nucleic acid amplification employ an enzyme that catalyzes homologous recombination and a polymerase. In some embodiments, methods for nucleic acid amplification can be conducted in a single reaction vessel. In some embodiments, methods for nucleic acid amplification can be conducted in a single continuous liquid phase of a reaction mixture, without need for compartmentalization of the reaction mixture or immobilization of reaction components. In some embodiments, methods for nucleic acid amplification comprise a amplifying at least one polynucleotide onto a surface under isothermal amplification conditions, optionally in the presence of a polymer. The polymer can include a sieving agent and/or a diffusion-reducing agent.

    METHODS FOR THE ANALYSIS OF PROXIMITY BINDING ASSAY DATA
    4.
    发明申请
    METHODS FOR THE ANALYSIS OF PROXIMITY BINDING ASSAY DATA 审中-公开

    公开(公告)号：US20190241942A1

    公开(公告)日：2019-08-08

    申请号：US16228304

    申请日：2018-12-20

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Shiaw-Min CHEN ,  David W. RUFF ,  Harrison LEONG

    IPC: C12Q1/6844 ,  G16B25/00 ,  G16B40/00

    CPC classification number: C12Q1/6844 ,  G16B25/00 ,  G16B40/00 ,  C12Q2545/114

    Abstract: Various embodiments of methods for analyzing proximity binding assay (PBA) data are disclosed. Proximity binding assays as a class of analyses offer the advantages of the sensitivity and specificity of biorecognition binding, along with the exponential signal amplification offered by a variety of oligonucleotide amplification reactions, such as the polymerase chain reaction (PCR). However, as various proximity binding assays have reaction kinetics governed by an additional step of the binding of a biorecognition probe (BRP) with a target molecule, there is a need for methods for the analysis of PBA data that are particularly suited to the unique characteristics of such data.

    NUCLEIC ACID AMPLIFICATION
    5.
    发明申请
    NUCLEIC ACID AMPLIFICATION 审中-公开

    公开(公告)号：US20190119738A1

    公开(公告)日：2019-04-25

    申请号：US16168810

    申请日：2018-10-23

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Chieh-Yuan LI ,  David RUFF ,  Jennifer O'NEIL ,  Rachel KASINSKAS ,  Shiaw-Min CHEN ,  Jonathan M. ROTHBERG ,  Bin LI ,  Kai Qin LAO

    IPC: C12Q1/6844 ,  C12Q1/686 ,  C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/6853

    Abstract: In some embodiments, provided are methods for nucleic acid amplification include subjecting the nucleic acid to be amplified to partially denaturing conditions. In some embodiments, methods for nucleic acid amplification include amplifying without fully denaturing the nucleic acid that is amplified. In some embodiments, the methods for nucleic acid amplification employ an enzyme that catalyzes homologous recombination and a polymerase. In some embodiments, methods for nucleic acid amplification can be conducted in a single reaction vessel. In some embodiments, methods for nucleic acid amplification can be conducted in a single continuous liquid phase of a reaction mixture, without need for compartmentalization of the reaction mixture or immobilization of reaction components. In some embodiments, methods for nucleic acid amplification comprise a amplifying at least one polynucleotide onto a surface under isothermal amplification conditions, optionally in the presence of a polymer. The polymer can include a sieving agent and/or a diffusion-reducing agent.

    NUCLEIC ACID AMPLIFICATION
    6.
    发明申请
    NUCLEIC ACID AMPLIFICATION 审中-公开

    公开(公告)号：US20140148345A1

    公开(公告)日：2014-05-29

    申请号：US14085718

    申请日：2013-11-20

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Chieh-Yuan LI ,  David RUFF ,  Jennifer O'NEIL ,  Rachel KASINSKAS ,  Shiaw-Min CHEN ,  Jonathan ROTHBERG

    IPC: C12Q1/68

    CPC classification number: C12Q1/6846 ,  C12Q1/6806 ,  C12Q1/6844 ,  C12Q1/6853 ,  C12Q1/686 ,  C12Q1/6874 ,  C12Q2531/119 ,  C12Q2565/537 ,  C12Q2563/159 ,  C12Q2565/513 ,  C12Q2565/519 ,  C12Q2565/607

    Abstract: In some embodiments, the present teachings provide methods for nucleic acid amplification, comprising forming a reaction mixture, and subjecting the reaction mixture to conditions suitable for nucleic acid amplification. In some embodiments, methods for nucleic acid amplification include subjecting the nucleic acid to be amplified to partially denaturing conditions. In some embodiments, methods for nucleic acid amplification include amplifying without fully denaturing the nucleic acid that is amplified. In some embodiments, the methods for nucleic acid amplification employ an enzyme that catalyzes homologous recombination and a polymerase. In some embodiments, methods for nucleic acid amplification can be conducted in a single reaction vessel. In some embodiments, methods for nucleic acid amplification can be conducted in a single continuous liquid phase of a reaction mixture, without need for compartmentalization of the reaction mixture or immobilization of reaction components. In some embodiments, methods for nucleic acid amplification comprise a amplifying at least one polynucleotide onto a surface under isothermal amplification conditions, optionally in the presence of a polymer. The polymer can include a sieving agent and/or a diffusion-reducing agent.

    NUCLEIC ACID AMPLIFICATION
    7.
    发明公开
    NUCLEIC ACID AMPLIFICATION 审中-公开

    公开(公告)号：US20240067939A1

    公开(公告)日：2024-02-29

    申请号：US18221099

    申请日：2023-07-12

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Chieh-Yuan LI ,  David RUFF ,  Shiaw-Min CHEN ,  Jennifer O'NEIL ,  Rachel KASINSKAS ,  Jonathan ROTHBERG ,  Bin LI ,  Kai Qin LAO

    IPC: C12N9/12 ,  C12Q1/6844 ,  C12Q1/6853 ,  C12Q1/6855 ,  C12Q1/686

    CPC classification number: C12N9/1252 ,  C12Q1/6846 ,  C12Q1/6853 ,  C12Q1/6855 ,  C12Q1/686 ,  C12Q1/6874

    Abstract: The present disclosure provides methods, compositions, kits and systems for nucleic acid amplification. In some embodiments, nucleic acid amplification methods include subjecting the nucleic acid to be amplified to partially denaturing conditions. In some embodiments, nucleic acid amplification methods include amplifying without fully denaturing the nucleic acid that is amplified. In some embodiments, the nucleic acid amplification method employs an enzyme that catalyzes homologous recombination and a polymerase. In some embodiments, methods for nucleic acid amplification can be conducted in a single reaction vessel and/or in a single continuous liquid phase of a reaction mixture, without need for compartmentalization of the reaction mixture or immobilization of reaction components. In some embodiments, methods for nucleic acid amplification comprise amplifying at least one polynucleotide onto a surface under isothermal amplification conditions, optionally in the presence of a polymer which can include a sieving agent and/or a diffusion-reducing agent.

    NUCLEIC ACID AMPLIFICATION
    10.
    发明申请
    NUCLEIC ACID AMPLIFICATION 有权
    核酸放大

    公开(公告)号：US20140080717A1

    公开(公告)日：2014-03-20

    申请号：US14023361

    申请日：2013-09-10

    Applicant: Life Technologies Corporation

    Inventor： Chieh-Yuan LI ,  David RUFF ,  Shiaw-Min CHEN ,  Jennifer O'NEIL ,  Rachel KASINSKAS ,  Jonathan ROTHBERG ,  Wolfgang HINZ

    IPC: C12Q1/68

    CPC classification number: C12N9/1252 ,  C12Q1/6846 ,  C12Q1/6853 ,  C12Q1/6855 ,  C12Q1/686 ,  C12Q1/6874 ,  C12Y207/07007 ,  C12Q2531/119 ,  C12Q2565/537

    Abstract: In some embodiments, the present teachings provide methods for nucleic acid amplification, comprising forming a reaction mixture, and subjecting the reaction mixture to conditions suitable for nucleic acid amplification. In some embodiments, methods for nucleic acid amplification include subjecting the nucleic acid to be amplified to partially denaturing conditions. In some embodiments, methods for nucleic acid amplification include amplifying without fully denaturing the nucleic acid that is amplified. In some embodiments, the methods for nucleic acid amplification employ an enzyme that catalyzes homologous recombination and a polymerase. In some embodiments, methods for nucleic acid amplification can be conducted in a single reaction vessel. In some embodiments, methods for nucleic acid amplification can be conducted in a single continuous liquid phase of a reaction mixture, without need for compartmentalization of the reaction mixture or immobilization of reaction components. In some embodiments, methods for nucleic acid amplification comprise a amplifying at least one polynucleotide onto a surface under isothermal amplification conditions, optionally in the presence of a polymer. The polymer can include a sieving agent and/or a diffusion-reducing agent.

    Abstract translation: 在一些实施方案中，本教导提供核酸扩增方法，包括形成反应混合物，并使反应混合物经受适合于核酸扩增的条件。 在一些实施方案中，用于核酸扩增的方法包括将待扩增的核酸进行部分变性的条件。 在一些实施方案中，用于核酸扩增的方法包括扩增而不完全变性扩增的核酸。 在一些实施方案中，用于核酸扩增的方法使用催化同源重组的酶和聚合酶。 在一些实施方案中，用于核酸扩增的方法可以在单个反应容器中进行。 在一些实施方案中，用于核酸扩增的方法可以在反应混合物的单个连续液相中进行，而不需要分隔反应混合物或固定反应组分。 在一些实施方案中，用于核酸扩增的方法包括在等温扩增条件下任选地在聚合物存在下在表面上扩增至少一种多核苷酸。 聚合物可以包括筛分剂和/或扩散还原剂。

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