公开(公告)号：US11248256B2

公开(公告)日：2022-02-15

申请号：US15614582

申请日：2017-06-05

Applicant: Life Technologies Corporation

Inventor： Robert Green ,  Dennis Wang ,  Julio Mulero ,  Robert Lagace

IPC: C12Q1/6827 ,  C12Q1/6888 ,  C12Q1/686 ,  C07H21/04

Abstract: Disclosed are primer set compositions, methods and kits for human identification using the highly complex sequence locus, SE33 (ACTBP2) in single and multiplex PCR reactions. Additionally, disclosed are three newly discovered single nucleotide polymorphisms (SNPs) within the SE33 locus that can cause discordance seen as mobility shift or allelic dropout. Also disclosed are kits useful in human identification.

公开(公告)号：US11913066B2

公开(公告)日：2024-02-27

申请号：US17585161

申请日：2022-01-26

Applicant: Life Technologies Corporation

Inventor： Robert Green ,  Dennis Wang ,  Julio Mulero ,  Robert Lagace

IPC: C12Q1/6827 ,  C12Q1/686 ,  C12Q1/6888

CPC classification number: C12Q1/6827 ,  C12Q1/6888 ,  C12Q1/686 ,  C12Q2537/143 ,  C12Q2600/156

Abstract: Disclosed are primer set compositions, methods and kits for human identification using the highly complex sequence locus, SE33 (ACTBP2) in single and multiplex PCR reactions. Additionally, disclosed are three newly discovered single nucleotide polymorphisms (SNPs) within the SE33 locus that can cause discordance seen as mobility shift or allelic dropout. Also disclosed are kits useful in human identification.

公开(公告)号：US09260757B2

公开(公告)日：2016-02-16

申请号：US14708467

申请日：2015-05-11

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Julio Mulero ,  Michael Malicdem ,  Robert Lagace

IPC: C12Q1/68

CPC classification number: C12Q1/6883 ,  C12Q1/6858 ,  C12Q1/6888 ,  C12Q2525/117 ,  C12Q2563/107 ,  C12Q2600/156

Abstract: Disclosed are methods for human identification utilizing newly discovered single nucleotide polymorphisms (SNPs) within CODIS loci which can cause allelic dropout. Also disclosed are kits useful in human identification.

Abstract translation: 本发明公开了利用新近发现的CODIS基因座内单核苷酸多态性（SNPs）进行人类鉴定的方法，可以导致等位基因脱落。 还公开了可用于人类鉴定的试剂盒。

公开(公告)号：US20240336958A1

公开(公告)日：2024-10-10

申请号：US18412907

申请日：2024-01-15

Applicant: Life Technologies Corporation

Inventor： Robert Green ,  Dennis Wang ,  Julio Mulero ,  Robert Lagace

IPC: C12Q1/6827 ,  C12Q1/686 ,  C12Q1/6888

CPC classification number: C12Q1/6827 ,  C12Q1/6888 ,  C12Q1/686 ,  C12Q2537/143 ,  C12Q2600/156

Abstract: Disclosed are primer set compositions, methods and kits for human identification using the highly complex sequence locus, SE33 (ACTBP2) in single and multiplex PCR reactions. Additionally, disclosed are three newly discovered single nucleotide polymorphisms (SNPs) within the SE33 locus that can cause discordance seen as mobility shift or allelic dropout. Also disclosed are kits useful in human identification.

公开(公告)号：US20160154990A1

公开(公告)日：2016-06-02

申请号：US14865079

申请日：2015-09-25

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Michael Harrold ,  Lori Hennessy ,  Robert Lagace

IPC: G06K9/00

CPC classification number: G06K9/00013 ,  A61B5/1172 ,  A61B5/1174 ,  A61B5/1176 ,  A61B5/14546 ,  A61B5/150022 ,  A61B5/150099 ,  A61B5/150229 ,  A61B5/150267 ,  A61B5/150305 ,  A61B5/150396 ,  A61B5/150404 ,  A61B5/150419 ,  A61B5/150427 ,  A61B5/150435 ,  A61B5/150511 ,  A61B5/150748 ,  A61B5/150755 ,  A61B5/150786 ,  A61B5/150847 ,  A61B5/150954 ,  A61B5/15109 ,  A61B5/1519 ,  A61B5/157 ,  A61B10/0045 ,  A61B10/02 ,  G05B1/00 ,  G06K9/00006 ,  G06K9/0008 ,  G06K9/00107 ,  G06K9/00892 ,  G06K2209/07

Abstract: Disclosed are devices and methods for collection and analyzing biological samples containing nucleic acid in conjunction with collecting at least one ridge and valley signature of a test subject, while keeping the sample and signature associated with each other. Such devices and methods are used in forensic, human identification, screening, and access control technologies to rapidly process an individual's identity or determine the identity of an individual.

公开(公告)号：US20160010146A1

公开(公告)日：2016-01-14

申请号：US14809418

申请日：2015-07-27

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Robert GREEN ,  Dennis Wang ,  Julio Mulero ,  Robert Lagace

IPC: C12Q1/68

CPC classification number: C12Q1/6827 ,  C12Q1/686 ,  C12Q1/6888 ,  C12Q2537/143 ,  C12Q2600/156

Abstract: Disclosed are primer set compositions, methods and kits for human identification using the highly complex sequence locus, SE33 (ACTBP2) in single and multiplex PCR reactions. Additionally, disclosed are three newly discovered single nucleotide polymorphisms (SNPs) within the SE33 locus that can cause discordance seen as mobility shift or allelic dropout. Also disclosed are kits useful in human identification.

Abstract translation: 公开了使用高度复杂的序列位点SE33（ACTBP2）在单次和多重PCR反应中进行人类鉴定的引物组合物，方法和试剂盒。 另外，所公开的是在SE33基因座内的三种新发现的单核苷酸多态性（SNP），其可以导致视为移动性偏移或等位基因缺失的不一致。 还公开了可用于人类鉴定的试剂盒。

公开(公告)号：US20150322522A1

公开(公告)日：2015-11-12

申请号：US14708467

申请日：2015-05-11

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Julio MULERO ,  Michael Malicdem ,  Robert Lagace

IPC: C12Q1/68

CPC classification number: C12Q1/6883 ,  C12Q1/6858 ,  C12Q1/6888 ,  C12Q2525/117 ,  C12Q2563/107 ,  C12Q2600/156

Abstract: Disclosed are methods for human identification utilizing newly discovered single nucleotide polymorphisms (SNPs) within CODIS loci which can cause allelic dropout. Also disclosed are kits useful in human identification.

Abstract translation: 本发明公开了利用新近发现的CODIS基因座单核苷酸多态性（SNPs）进行人类鉴定的方法，可以导致等位基因脱落。 还公开了可用于人类鉴定的试剂盒。

公开(公告)号：US20150167068A1

公开(公告)日：2015-06-18

申请号：US14414532

申请日：2013-07-15

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Robert Lagace

IPC: C12Q1/68

CPC classification number: C12Q1/6855 ,  C12Q1/6853 ,  C12Q1/6888 ,  C12Q2521/531 ,  C12Q2525/131 ,  C12Q2537/143

Abstract: The present invention provides methods, compositions, kits, systems and apparatus that are useful for multiplex PCR of one or more nucleic acids which belong to a panel of single nucleotide polymorphisms (SNPs) useful to identify a human. In particular, various target-specific primers are provided that allow for the selective amplification of one or more target sequences in the panel. In some aspects, amplified target sequences obtained using the disclosed methods, kits, systems and apparatuses can be used in various downstream processes including nucleic acid sequencing and used to identify a human.

Abstract translation: 本发明提供了可用于多属性PCR的一种或多种核酸的方法，组合物，试剂盒，系统和装置，所述核酸属于可用于鉴定人的单核苷酸多态性（SNP）的一组。 特别地，提供允许在面板中选择性扩增一个或多个靶序列的各种靶特异性引物。 在一些方面，使用所公开的方法，试剂盒，系统和装置获得的扩增靶序列可用于包括核酸测序在内的各种下游过程并用于鉴定人。