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    STABLE COMPOSITIONS FOR NUCLEIC ACID AMPLIFICATION AND SEQUENCING
    1.
    发明申请
    STABLE COMPOSITIONS FOR NUCLEIC ACID AMPLIFICATION AND SEQUENCING 审中-公开
    用于核酸扩增和测序的稳定组合物

    公开(公告)号：US20150050660A1

    公开(公告)日：2015-02-19

    申请号：US14466577

    申请日：2014-08-22

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Ayoub RASHTCHIAN ,  Joseph Solus

    IPC: C12N9/12 ,  C12Q1/68 ,  C12P19/34

    CPC classification number: C12N9/1252 ,  C12N9/96 ,  C12P19/34 ,  C12Q1/686 ,  C12Q1/6869 ,  C12Q2527/149 ,  C12Q2527/137 ,  C12Q2527/125

    Abstract: The present invention is directed to compositions comprising mixtures of reagents, including thermostable enzymes (e.g., thermostable DNA polymerases), buffers, cofactors and other components, suitable for immediate use in nucleic acid amplification or sequencing techniques without dilution or addition of further components. The compositions contain no stablizing agents (e.g., glycerol or serum albumin) and unexpectedly maintain activity for extended periods of time upon storage at temperatures above freezing. These compositions are useful, alone or in the form of kits, for nucleic acid amplification (e.g., by the Polymerase Chain Reaction) and sequencing (e.g., by dideoxy or “Sanger” sequencing), or for any procedure utilizing thermostable DNA polymerases in a variety of medical, forensic and agricultural applications. In particular, the compositions and methods are useful for amplifying and sequencing nucleic acid molecules that are larger than about 7 kilobases in size.

    Abstract translation: 本发明涉及包含试剂混合物的组合物，包括热稳定酶（例如热稳定的DNA聚合酶），缓冲液，辅因子和其它组分，适于立即用于核酸扩增或测序技术而不稀释或添加其它组分。 组合物不含稳定剂（例如甘油或血清白蛋白），并且在高于冰点的温度下储存时，意外地保持活性延长的时间。 这些组合物单独或以试剂盒的形式用于核酸扩增（例如，通过聚合酶链式反应）和测序（例如，通过双脱氧或“Sanger”测序），或者对于使用热稳定性DNA聚合酶 各种医疗，法医和农业应用。 特别地，组合物和方法可用于扩增和测序尺寸大于约7千碱基的核酸分子。

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