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    Compositions and methods for reverse transcriptase-polymerase chain reaction (RT-PCR)
    2.
    发明授权
    Compositions and methods for reverse transcriptase-polymerase chain reaction (RT-PCR) 有权

    公开(公告)号：US09873901B2

    公开(公告)日：2018-01-23

    申请号：US14498827

    申请日：2014-09-26

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Jun Lee ,  Ayoub Rashtchian

    IPC: C12P19/34 ,  C12N15/10 ,  C12Q1/68 ,  C12N9/12

    CPC classification number: C12P19/34 ,  C12N9/1276 ,  C12N15/1096 ,  C12Q1/6846 ,  C12Q2527/149 ,  C12Q2527/125 ,  C12Q2521/107

    Abstract: The present invention is directed to compositions and methods useful for the amplification of nucleic acid molecules by reverse transcriptase-polymerase chain reaction (RT-PCR). Specifically, the invention provides compositions and methods for the amplification of nucleic acid molecules in a simplified one- or two-step RT-PCR procedure using combinations of reverse transcriptase and thermostable DNA polymerase enzymes in conjunction with sulfur-containing molecules or acetate-containing molecules (or combinations of such sulfur-containing molecules and acetate-containing molecules), and optionally bovine serum albumin. The invention thus facilitates the rapid and efficient amplification of nucleic acid molecules and the detection and quantitation of RNA molecules. The invention also is useful in the rapid production and amplification of cDNAs which may be used for a variety of industrial, medical and forensic purposes.

    Compositions and methods for reverse transcriptase-polymerase chain reaction (RT-PCR)
    3.
    发明授权
    Compositions and methods for reverse transcriptase-polymerase chain reaction (RT-PCR) 有权
    逆转录聚合酶链反应（RT-PCR）的组合物和方法

    公开(公告)号：US09556466B2

    公开(公告)日：2017-01-31

    申请号：US13764754

    申请日：2013-02-11

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Jun Lee ,  Ayoub Rashtchian

    IPC: C12P19/34 ,  C12N15/10 ,  C12Q1/68 ,  C12N9/12

    CPC classification number: C12P19/34 ,  C12N9/1276 ,  C12N15/1096 ,  C12Q1/6846 ,  C12Q2527/149 ,  C12Q2527/125 ,  C12Q2521/107

    Abstract: The present invention is directed to compositions and methods useful for the amplification of nucleic acid molecules by reverse transcriptase-polymerase chain reaction (RT-PCR). Specifically, the invention provides compositions and methods for the amplification of nucleic acid molecules in a simplified one- or two-step RT-PCR procedure using combinations of reverse transcriptase and thermostable DNA polymerase enzymes in conjunction with sulfur-containing molecules or acetate-containing molecules (or combinations of such sulfur-containing molecules and acetate-containing molecules), and optionally bovine serum albumin. The invention thus facilitates the rapid and efficient amplification of nucleic acid molecules and the detection and quantitation of RNA molecules. The invention also is useful in the rapid production and amplification of cDNAs which may be used for a variety of industrial, medical and forensic purposes.

    Abstract translation: 本发明涉及通过逆转录酶 - 聚合酶链反应（RT-PCR）扩增核酸分子的组合物和方法。 具体地，本发明提供了使用逆转录酶和热稳定DNA聚合酶与含硫分子或含乙酸酯分子结合的简化的一步或两步RT-PCR方法扩增核酸分子的组合物和方法 （或这种含硫分子和含乙酸酯的分子的组合）和任选的牛血清白蛋白。 因此，本发明促进了核酸分子的快速和有效的扩增以及RNA分子的检测和定量。 本发明也可用于快速生产和扩增可用于各种工业，医学和法医目的的cDNA。

    COMPOSITIONS AND METHODS FOR REVERSE TRANSCRIPTASE-POLYMERASE CHAIN REACTION (RT-PCR)
    7.
    发明申请
    COMPOSITIONS AND METHODS FOR REVERSE TRANSCRIPTASE-POLYMERASE CHAIN REACTION (RT-PCR) 有权

    公开(公告)号：US20130164788A1

    公开(公告)日：2013-06-27

    申请号：US13764754

    申请日：2013-02-11

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Jun LEE ,  Ayoub Rashtchian

    IPC: C12P19/34

    CPC classification number: C12P19/34 ,  C12N9/1276 ,  C12N15/1096 ,  C12Q1/6846 ,  C12Q2527/149 ,  C12Q2527/125 ,  C12Q2521/107

    Abstract: The present invention is directed to compositions and methods useful for the amplification of nucleic acid molecules by reverse transcriptase-polymerase chain reaction (RT-PCR). Specifically, the invention provides compositions and methods for the amplification of nucleic acid molecules in a simplified one- or two-step RT-PCR procedure using combinations of reverse transcriptase and thermostable DNA polymerase enzymes in conjunction with sulfur-containing molecules or acetate-containing molecules (or combinations of such sulfur-containing molecules and acetate-containing molecules), and optionally bovine serum albumin. The invention thus facilitates the rapid and efficient amplification of nucleic acid molecules and the detection and quantitation of RNA molecules. The invention also is useful in the rapid production and amplification of cDNAs which may be used for a variety of industrial, medical and forensic purposes.

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