公开(公告)号：US09580743B2

公开(公告)日：2017-02-28

申请号：US14450925

申请日：2014-08-04

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jun Lee ,  Robert Potter ,  David Mandelman

IPC: C12N15/62 ,  C12Q1/68 ,  C12N9/12

CPC classification number: C12Q1/6806 ,  C12N9/1252 ,  C12N9/1276 ,  C12N15/62 ,  C12Q1/6848 ,  C12Q2522/101

Abstract: Fusion proteins comprising a single strand DNA binding protein and a nucleic acid polymerase (e.g. DNA polymerase or reverse transcriptase). These high fidelity proteins are suitable for use in nucleic acid amplification methods, including the polymerase chain reaction (PCR).

Abstract translation: 包含单链DNA结合蛋白和核酸聚合酶（例如DNA聚合酶或逆转录酶）的融合蛋白。 这些高保真蛋白质适用于核酸扩增方法，包括聚合酶链反应（PCR）。

公开(公告)号：US09556466B2

公开(公告)日：2017-01-31

申请号：US13764754

申请日：2013-02-11

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jun Lee ,  Ayoub Rashtchian

IPC: C12P19/34 ,  C12N15/10 ,  C12Q1/68 ,  C12N9/12

CPC classification number: C12P19/34 ,  C12N9/1276 ,  C12N15/1096 ,  C12Q1/6846 ,  C12Q2527/149 ,  C12Q2527/125 ,  C12Q2521/107

Abstract: The present invention is directed to compositions and methods useful for the amplification of nucleic acid molecules by reverse transcriptase-polymerase chain reaction (RT-PCR). Specifically, the invention provides compositions and methods for the amplification of nucleic acid molecules in a simplified one- or two-step RT-PCR procedure using combinations of reverse transcriptase and thermostable DNA polymerase enzymes in conjunction with sulfur-containing molecules or acetate-containing molecules (or combinations of such sulfur-containing molecules and acetate-containing molecules), and optionally bovine serum albumin. The invention thus facilitates the rapid and efficient amplification of nucleic acid molecules and the detection and quantitation of RNA molecules. The invention also is useful in the rapid production and amplification of cDNAs which may be used for a variety of industrial, medical and forensic purposes.

Abstract translation: 本发明涉及通过逆转录酶 - 聚合酶链反应（RT-PCR）扩增核酸分子的组合物和方法。 具体地，本发明提供了使用逆转录酶和热稳定DNA聚合酶与含硫分子或含乙酸酯分子结合的简化的一步或两步RT-PCR方法扩增核酸分子的组合物和方法 （或这种含硫分子和含乙酸酯的分子的组合）和任选的牛血清白蛋白。 因此，本发明促进了核酸分子的快速和有效的扩增以及RNA分子的检测和定量。 本发明也可用于快速生产和扩增可用于各种工业，医学和法医目的的cDNA。

公开(公告)号：US20160272955A1

公开(公告)日：2016-09-22

申请号：US15173439

申请日：2016-06-03

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Weidong ZHENG ,  Jun Lee ,  Robert Potter ,  David Mandelman

IPC: C12N9/12 ,  C12Q1/68

CPC classification number: C12N9/1252 ,  C12N9/1276 ,  C12P19/34 ,  C12Q1/6806 ,  C12Q1/686 ,  C12Y207/07007 ,  Y02P20/52 ,  C12Q2561/101 ,  C12Q2527/149 ,  C12Q2521/101

Abstract: A thermostable DNA polymerase composition comprising at least two DNA polymerases, one of which is substantially reduced in 5′-exonuclease activity and one of which has 5′-exonuclease activity. This polymerase may be used in methods including, but not limited to, nucleic acid synthesis, DNA sequencing, nucleic acid amplification and cDNA synthesis.

Abstract translation: 一种热稳定DNA聚合酶组合物，其包含至少两种DNA聚合酶，其中之一基本上降低了5'-核酸外切酶活性，其中一种具有5'-核酸外切酶活性。 该聚合酶可用于包括但不限于核酸合成，DNA测序，核酸扩增和cDNA合成的方法。

公开(公告)号：US09873901B2

公开(公告)日：2018-01-23

申请号：US14498827

申请日：2014-09-26

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jun Lee ,  Ayoub Rashtchian

IPC: C12P19/34 ,  C12N15/10 ,  C12Q1/68 ,  C12N9/12

CPC classification number: C12P19/34 ,  C12N9/1276 ,  C12N15/1096 ,  C12Q1/6846 ,  C12Q2527/149 ,  C12Q2527/125 ,  C12Q2521/107

Abstract: The present invention is directed to compositions and methods useful for the amplification of nucleic acid molecules by reverse transcriptase-polymerase chain reaction (RT-PCR). Specifically, the invention provides compositions and methods for the amplification of nucleic acid molecules in a simplified one- or two-step RT-PCR procedure using combinations of reverse transcriptase and thermostable DNA polymerase enzymes in conjunction with sulfur-containing molecules or acetate-containing molecules (or combinations of such sulfur-containing molecules and acetate-containing molecules), and optionally bovine serum albumin. The invention thus facilitates the rapid and efficient amplification of nucleic acid molecules and the detection and quantitation of RNA molecules. The invention also is useful in the rapid production and amplification of cDNAs which may be used for a variety of industrial, medical and forensic purposes.