公开(公告)号：US20210214787A1

公开(公告)日：2021-07-15

申请号：US17217492

申请日：2021-03-30

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Guobin LUO ,  Marina SEDOVA ,  David LIGHT ,  Ryan JONES ,  Mohammad ALANJARY

IPC: C12Q1/6874 ,  C12Q1/6806

Abstract: A method for depositing particles on a surface includes receiving a plurality of particles, a particle of the plurality of particles having a polymer matrix conjugated to nucleic acid strands, exposing the plurality of particles to a solution; and applying the plurality of particles to a surface following exposing the plurality of particles to the solution, particles of the plurality of particles depositing on the surface. The solution includes a magnesium salt in a range of 30 mM to 500 mM, a potassium salt in a range of 0.8 M to 1.0 M, a buffering agent in a range of 150 mM to 500 mM, and a surfactant in a range of 0.05% to 0.5%.

公开(公告)号：US20180223357A1

公开(公告)日：2018-08-09

申请号：US15785579

申请日：2017-10-17

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Guobin LUO ,  Marina SEDOVA ,  David LIGHT ,  Ryan JONES ,  Mohammad ALANJARY

IPC: C12Q1/6874 ,  C12Q1/6806

CPC classification number: C12Q1/6874 ,  C12Q1/6806 ,  C12Q2527/125 ,  C12Q2563/149 ,  C12Q2563/137 ,  C12Q2535/122

Abstract: A method of sequencing a nucleic acid strand includes receiving particles having nucleic acid strands coupled to a polymer matrix, exposing the particles to a solution including a condensing agent, and applying the particles to a surface, the particles depositing on the surface.