公开(公告)号：US20170174846A1

公开(公告)日：2017-06-22

申请号：US15392834

申请日：2016-12-28

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Wolfgang HINZ ,  Ryan JONES ,  David LIGHT

IPC: C08J3/12 ,  B07B1/00 ,  C12N15/10

CPC classification number: C08J3/12 ,  B07B1/00 ,  C08J2300/14 ,  C12N15/1017

Abstract: A method of preparing hydrogel particles includes applying a solution including a plurality of hydrogel particles to a stir cell. A retentate side of a filter defines a lower surface of the stir cell. The filter has the retentate side and a permeate side. The method further includes, while stirring the solution within the stir cell, dispensing a buffer solution at a first flow rate to the stir cell and drawing a permeate from the permeate side of the filter using a pump at a second flow rate, the permeate including a subset of the plurality of hydrogel particles.

公开(公告)号：US20210139949A1

公开(公告)日：2021-05-13

申请号：US17027206

申请日：2020-09-21

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Kristopher BARBEE ,  Ryan JONES ,  Sean MCCUSKER ,  Maximilian CARPINO ,  John LEAMON ,  Jonathan SCHULTZ

IPC: C12Q1/6806 ,  B04B13/00 ,  B04B5/04 ,  G01N35/00 ,  G01N35/10 ,  C12Q1/6869

Abstract: A sample preparation apparatus includes a robotic system providing movement in three orthogonal directions to an arm operable to receive a pipette tip and to facilitate movement of fluid into and out of the pipette tip. Optionally, the robot can include a gripper arm in addition to the pipette receiving arm. In addition, the sample preparation apparatus can include a tray for receiving pipette tips, receptacles for receiving tubes, an apparatus for forming an emulsion, a device for forming particles that include copies of the polynucleotide, a device for enriching the particles, as well as a centrifuge for loading such particles onto a sensor array. The sample preparation apparatus can further include receptacles for holding containers of reagent solutions.

公开(公告)号：US20210214787A1

公开(公告)日：2021-07-15

申请号：US17217492

申请日：2021-03-30

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Guobin LUO ,  Marina SEDOVA ,  David LIGHT ,  Ryan JONES ,  Mohammad ALANJARY

IPC: C12Q1/6874 ,  C12Q1/6806

Abstract: A method for depositing particles on a surface includes receiving a plurality of particles, a particle of the plurality of particles having a polymer matrix conjugated to nucleic acid strands, exposing the plurality of particles to a solution; and applying the plurality of particles to a surface following exposing the plurality of particles to the solution, particles of the plurality of particles depositing on the surface. The solution includes a magnesium salt in a range of 30 mM to 500 mM, a potassium salt in a range of 0.8 M to 1.0 M, a buffering agent in a range of 150 mM to 500 mM, and a surfactant in a range of 0.05% to 0.5%.

公开(公告)号：US20150057377A1

公开(公告)日：2015-02-26

申请号：US14528138

申请日：2014-10-30

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Ryan JONES ,  Maximilian CARPINO

IPC: B01D17/04 ,  C07H21/00

CPC classification number: B01D17/047 ,  B01D21/00 ,  B01D21/0009 ,  C07H21/00 ,  C10G33/04 ,  C12Q1/68

Abstract: A method for breaking emulsions includes applying a polymer mixture to an emulsion. The emulsion can be energized, such as through centrifugation or vibration. In particular, the polymer mixture can be in liquid form. The polymer mixture includes first and second liquid polymer, the second liquid polymer being less hydrophilic than the first liquid polymer. In a water-in-oil emulsion, the less hydrophilic polymer can preferentially reside within the oil phase.

Abstract translation: 用于破乳的方法包括将聚合物混合物施加到乳液上。 乳液可以被通电，例如通过离心或振动。 特别地，聚合物混合物可以是液体形式。 聚合物混合物包括第一和第二液体聚合物，第二液体聚合物的亲水性低于第一液体聚合物。 在油包水乳液中，亲水性较差的聚合物优先存在于油相中。

公开(公告)号：US20180223357A1

公开(公告)日：2018-08-09

申请号：US15785579

申请日：2017-10-17

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Guobin LUO ,  Marina SEDOVA ,  David LIGHT ,  Ryan JONES ,  Mohammad ALANJARY

IPC: C12Q1/6874 ,  C12Q1/6806

CPC classification number: C12Q1/6874 ,  C12Q1/6806 ,  C12Q2527/125 ,  C12Q2563/149 ,  C12Q2563/137 ,  C12Q2535/122

Abstract: A method of sequencing a nucleic acid strand includes receiving particles having nucleic acid strands coupled to a polymer matrix, exposing the particles to a solution including a condensing agent, and applying the particles to a surface, the particles depositing on the surface.

公开(公告)号：US20160230214A1

公开(公告)日：2016-08-11

申请号：US15133428

申请日：2016-04-20

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Kristopher BARBEE ,  Ryan JONES ,  Sean MCCUSKER ,  Maximilian CARPINO ,  John LEAMON ,  Jonathan SCHULTZ

IPC: C12Q1/68 ,  B04B5/04

Abstract: An apparatus includes a robotic system providing movement in three orthogonal directions to an arm operable to receive a pipette tip and to facilitate movement of fluid into and out of the pipette tip. In addition, the apparatus can include a tray for receiving pipette tips, receptacles for receiving tubes, an apparatus for forming an emulsion, a device for forming particles that include copies of the polynucleotide, a device for enriching the particles and an apparatus for loading such particles onto a sensor array. The apparatus can further include receptacles for holding containers of reagent solutions. Optionally, the robot can include a gripper arm in addition to the pipette receiving arm.

Abstract translation: 一种装置包括机器人系统，该机器人系统在三个正交方向上提供可操作以接收移液管尖端的臂并促进流体进入和移出移液管尖端的运动。 此外，该装置可以包括用于接收移液管吸头的托盘，用于接收管的容器，用于形成乳液的装置，用于形成包括多核苷酸的拷贝的颗粒的装置，用于富集颗粒的装置和用于加载颗粒的装置 颗粒到传感器阵列上。 该装置还可以包括容纳试剂溶液容器的容器。 可选地，除了移液管接收臂之外，机器人还可以包括夹持臂。