公开(公告)号：US20180282797A1

公开(公告)日：2018-10-04

申请号：US15927572

申请日：2018-03-21

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Carole BORNARTH ,  Michael LAU ,  Junko STEVENS

IPC: C12Q1/6848 ,  C12Q1/686 ,  C07K16/00 ,  C12N9/00

Abstract: The present disclosure is directed to compositions, methods and kits for amplifying target nucleic acids while reducing non-specific amplification and undesired amplification products using a dual hot start reaction mixture that comprise at least two different hot start mechanisms.

公开(公告)号：US20200370109A1

公开(公告)日：2020-11-26

申请号：US16947727

申请日：2020-08-13

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Carole BORNARTH ,  Michael LAU ,  Junko STEVENS

IPC: C12Q1/6848 ,  C12N9/00 ,  C07K16/00 ,  C12Q1/686

Abstract: The present disclosure is directed to compositions, methods and kits for amplifying target nucleic acids while reducing non-specific amplification and undesired amplification products using a dual hot start reaction mixture that comprise at least two different hot start mechanisms.

公开(公告)号：US20240182958A1

公开(公告)日：2024-06-06

申请号：US18465581

申请日：2023-09-12

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Carole BORNARTH ,  Michael LAU ,  Junko STEVENS

IPC: C12Q1/6848 ,  C07K16/00 ,  C12N9/00 ,  C12Q1/686

CPC classification number: C12Q1/6848 ,  C07K16/00 ,  C12N9/00 ,  C12Q1/686

Abstract: The present disclosure is directed to compositions, methods and kits for amplifying target nucleic acids while reducing non-specific amplification and undesired amplification products using a dual hot start reaction mixture that comprise at least two different hot start mechanisms.

公开(公告)号：US20140099644A1

公开(公告)日：2014-04-10

申请号：US13918768

申请日：2013-06-14

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Carole BORNARTH ,  Michael LAU ,  Junko STEVENS

IPC: C12Q1/68

CPC classification number: C12Q1/6848 ,  C07K16/00 ,  C12N9/00 ,  C12Q1/686 ,  C12Q2527/125 ,  C12Q2547/107 ,  C12Q2549/101

Abstract: The present disclosure is directed to compositions, methods and kits for amplifying target nucleic acids while reducing non-specific amplification and undesired amplification products using a dual hot start reaction mixture that comprise at least two different hot start mechanisms.

Abstract translation: 本公开涉及用于扩增靶核酸的组合物，方法和试剂盒，同时使用包含至少两种不同热启动机制的双重热启动反应混合物减少非特异性扩增和不期望的扩增产物。