公开(公告)号：US20200370109A1

公开(公告)日：2020-11-26

申请号：US16947727

申请日：2020-08-13

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Carole BORNARTH ,  Michael LAU ,  Junko STEVENS

IPC: C12Q1/6848 ,  C12N9/00 ,  C07K16/00 ,  C12Q1/686

Abstract: The present disclosure is directed to compositions, methods and kits for amplifying target nucleic acids while reducing non-specific amplification and undesired amplification products using a dual hot start reaction mixture that comprise at least two different hot start mechanisms.

公开(公告)号：US20180044371A1

公开(公告)日：2018-02-15

申请号：US15682103

申请日：2017-08-21

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Tom XU ,  Christopher TRINH ,  Carole BORNARTH ,  Brian EVANS ,  Mousumi RATH ,  Kathy TRAN

IPC: C07H21/02 ,  C12Q1/68 ,  C12N15/10

CPC classification number: C07H21/02 ,  C12N15/1006 ,  C12Q1/6809 ,  C12Q1/6834 ,  C12Q1/6876 ,  C12Q2600/178 ,  C12Q2525/207 ,  C12Q2563/149

Abstract: Method and compositions and kits for isolation, identification, and quantification of miRNAs and other small RNAs, including but not limited to, siRNAs, mRNAs, and snRNAs are disclosed. Methods of diagnosing a disease or its progression are also disclosed.

公开(公告)号：US20210323994A1

公开(公告)日：2021-10-21

申请号：US17304883

申请日：2021-06-28

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Tom XU ,  Christopher TRINH ,  Carole BORNARTH ,  Brian EVANS ,  Mousumi RATH ,  Kathy TRAN

IPC: C07H21/02 ,  C12Q1/6876 ,  C12N15/10 ,  C12Q1/6809 ,  C12Q1/6834

Abstract: Method and compositions and kits for isolation, identification, and quantification of miRNAs and other small RNAs, including but not limited to, siRNAs, mRNAs, and snRNAs are disclosed. Methods of diagnosing a disease or its progression are also disclosed.

公开(公告)号：US20140315190A1

公开(公告)日：2014-10-23

申请号：US14213204

申请日：2014-03-14

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Carole BORNARTH ,  Mousumi RATH

IPC: G01N33/557 ,  G01N33/58

CPC classification number: G01N33/582 ,  C09B23/10 ,  C09B23/105 ,  C09B23/107 ,  C09B23/145 ,  G01N33/6803

Abstract: According to the present teachings, compositions, kits, and methods for protein melt analysis are provided that utilizing one or more fluorophore dyes. In some embodiments, a method comprises preparing a sample by mixing at least one protein with two or more dyes, and applying a controlled heating, while recording the fluorescence emission of the sample. The methods can be used, for example, for screening conditions for optimized protein stability, screening for ligands that bind and enhance protein stability (e.g., protein-protein interactions), screening for mutations for enhanced stability, screening crystallization conditions for protein stability, screening storage conditions for protein stability, and screening conditions in which a protein will be used (e.g., production conditions, treatment conditions, etc.) for protein stability.

Abstract translation: 根据本教导，提供了利用一种或多种荧光团染料的蛋白质熔融分析的组合物，试剂盒和方法。 在一些实施方案中，方法包括通过将至少一种蛋白质与两种或更多种染料混合并在记录样品的荧光发射的同时施加受控加热来制备样品。 该方法可用于例如筛选优化蛋白质稳定性的条件，筛选结合并增强蛋白质稳定性的配体（例如，蛋白质 - 蛋白质相互作用），筛选突变以提高稳定性，筛选结晶条件以获得蛋白质稳定性，筛选 用于蛋白质稳定性的储存条件，以及蛋白质稳定性使用蛋白质的筛选条件（例如，生产条件，处理条件等）。

公开(公告)号：US20210063405A1

公开(公告)日：2021-03-04

申请号：US17016178

申请日：2020-09-09

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Carole BORNARTH ,  Mousumi RATH

IPC: G01N33/58 ,  G01N33/68 ,  C09B23/10 ,  C09B23/14

Abstract: According to the present teachings, compositions, kits, and methods for protein melt analysis are provided that utilizing one or more fluorophore dyes. In some embodiments, a method comprises preparing a sample by mixing at least one protein with two or more dyes, and applying a controlled heating, while recording the fluorescence emission of the sample. The methods can be used, for example, for screening conditions for optimized protein stability, screening for ligands that bind and enhance protein stability (e.g., protein-protein interactions), screening for mutations for enhanced stability, screening crystallization conditions for protein stability, screening storage conditions for protein stability, and screening conditions in which a protein will be used (e.g., production conditions, treatment conditions, etc.) for protein stability.

公开(公告)号：US20180282797A1

公开(公告)日：2018-10-04

申请号：US15927572

申请日：2018-03-21

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Carole BORNARTH ,  Michael LAU ,  Junko STEVENS

IPC: C12Q1/6848 ,  C12Q1/686 ,  C07K16/00 ,  C12N9/00

Abstract: The present disclosure is directed to compositions, methods and kits for amplifying target nucleic acids while reducing non-specific amplification and undesired amplification products using a dual hot start reaction mixture that comprise at least two different hot start mechanisms.

公开(公告)号：US20240182958A1

公开(公告)日：2024-06-06

申请号：US18465581

申请日：2023-09-12

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Carole BORNARTH ,  Michael LAU ,  Junko STEVENS

IPC: C12Q1/6848 ,  C07K16/00 ,  C12N9/00 ,  C12Q1/686

CPC classification number: C12Q1/6848 ,  C07K16/00 ,  C12N9/00 ,  C12Q1/686

Abstract: The present disclosure is directed to compositions, methods and kits for amplifying target nucleic acids while reducing non-specific amplification and undesired amplification products using a dual hot start reaction mixture that comprise at least two different hot start mechanisms.

公开(公告)号：US20180348230A1

公开(公告)日：2018-12-06

申请号：US16001613

申请日：2018-06-06

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Carole BORNARTH ,  Mousumi RATH

IPC: G01N33/58 ,  C09B23/14 ,  C09B23/10 ,  G01N33/68

CPC classification number: G01N33/582 ,  C09B23/10 ,  C09B23/105 ,  C09B23/107 ,  C09B23/145 ,  G01N33/6803

Abstract: According to the present teachings, compositions, kits, and methods for protein melt analysis are provided that utilizing one or more fluorophore dyes. In some embodiments, a method comprises preparing a sample by mixing at least one protein with two or more dyes, and applying a controlled heating, while recording the fluorescence emission of the sample. The methods can be used, for example, for screening conditions for optimized protein stability, screening for ligands that bind and enhance protein stability (e.g., protein-protein interactions), screening for mutations for enhanced stability, screening crystallization conditions for protein stability, screening storage conditions for protein stability, and screening conditions in which a protein will be used (e.g., production conditions, treatment conditions, etc.) for protein stability.

公开(公告)号：US20140099644A1

公开(公告)日：2014-04-10

申请号：US13918768

申请日：2013-06-14

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Carole BORNARTH ,  Michael LAU ,  Junko STEVENS

IPC: C12Q1/68

CPC classification number: C12Q1/6848 ,  C07K16/00 ,  C12N9/00 ,  C12Q1/686 ,  C12Q2527/125 ,  C12Q2547/107 ,  C12Q2549/101

Abstract: The present disclosure is directed to compositions, methods and kits for amplifying target nucleic acids while reducing non-specific amplification and undesired amplification products using a dual hot start reaction mixture that comprise at least two different hot start mechanisms.

Abstract translation: 本公开涉及用于扩增靶核酸的组合物，方法和试剂盒，同时使用包含至少两种不同热启动机制的双重热启动反应混合物减少非特异性扩增和不期望的扩增产物。