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公开(公告)号：US20190201530A1
公开(公告)日：2019-07-04
申请号：US16230393
申请日：2018-12-21
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Xavier de MOLLERAT du JEU , Nektaria ANDRONIKOU
IPC: A61K47/18 , A61K9/127 , A61K31/7105 , C12N15/88
CPC classification number: A61K47/186 , A61K9/1272 , A61K31/7105 , C12N15/88
Abstract: The present invention provides lipid aggregate compositions having at least two cationic lipids in combination with one or optionally more than one non-cationic lipids for the in vitro and/or in vivo delivery of biologically active molecules including nucleic acids, specifically DNA and RNA, and proteins into cells and tissues. Methods are also provided that use the compounds of the present invention to deliver biologically active molecules, into cells and tissues to facilitate the expression of target proteins therein. In some non-limiting embodiments, the subject lipid aggregate compositions can be used to deliver nucleic acid molecules to facilitate the expression of proteins involved in cellular reprogramming and genome editing applications.
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公开(公告)号：US20160045600A1
公开(公告)日：2016-02-18
申请号：US14800565
申请日：2015-07-15
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Xavier DE MOLLERAT DU JEU , Nektaria ANDRONIKOU
IPC: A61K47/18 , C12N15/88 , A61K31/7105
CPC classification number: A61K47/186 , A61K9/1272 , A61K31/7105 , C12N15/88
Abstract: The present invention provides lipid aggregate compositions having at least two cationic lipids in combination with one or optionally more than one non-cationic lipids for the in vitro and/or in vivo delivery of biologically active molecules including nucleic acids, specifically DNA and RNA, and proteins into cells and tissues. Methods are also provided that use the compounds of the present invention to deliver biologically active molecules, into cells and tissues to facilitate the expression of target proteins therein. In some non-limiting embodiments, the subject lipid aggregate compositions can be used to deliver nucleic acid molecules to facilitate the expression of proteins involved in cellular reprogramming and genome editing applications.
Abstract translation: 本发明提供具有至少两种阳离子脂质与一种或任选多种非阳离子脂质组合的脂质聚集体组合物，用于体外和/或体内递送生物活性分子，包括核酸，特别是DNA和RNA，以及 蛋白质进入细胞和组织。 还提供了使用本发明化合物将生物活性分子递送到细胞和组织中以促进其中靶蛋白表达的方法。 在一些非限制性实施方案中，本发明的脂质聚集体组合物可用于递送核酸分子以促进参与细胞重编程和基因组编辑应用的蛋白质的表达。
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公开(公告)号：US20210123009A1
公开(公告)日：2021-04-29
申请号：US17079100
申请日：2020-10-23
Inventor： Steven Huei YEO , Siu Wee HON , Han WEI , Yvonne PECK , Way Xuang LEE , Benyong SHI , Chee Wai CHAN , Beng Heng (Derrick) LIM , Dhanaraj SHANMUGAM , Stacey TEO , Jun Yan THAM , Kok Shyong CHONG , Li Yang LIM , Michael GORDON , Nektaria ANDRONIKOU , Chris ROSSMANN , Xavier DE Mollerat DU JEU , Ulrich FORKE
Abstract: The present disclosure provides electroporation cartridges for single use electroporation as well as electroporation cartridges for automated batch processing, electroporation instruments and systems and methods of electroporation using these devices and systems. In some embodiments, electroporation cartridges comprise an electroporation chamber defined by an elongate body, a first electrode at a proximal end and a second electrode at a distal end of a chamber. Electroporation systems of the disclosure include one or more components including a pulse generator, compartments for placing either flow-through or single use electroporation cartridges, components for storage of cells, cooling and pre-cooling mechanisms, removably insertable modular casings having compartments for holding and arranging electroporation system and reagent components, one or more pumps for moving sample through the system, and processors and controllers.
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公开(公告)号：US20210023225A1
公开(公告)日：2021-01-28
申请号：US17063677
申请日：2020-10-05
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Xavier de MOLLERAT du JEU , Nektaria ANDRONIKOU
IPC: A61K47/18 , A61K9/127 , A61K31/7105 , C12N15/88
Abstract: The present invention provides lipid aggregate compositions having at least two cationic lipids in combination with one or optionally more than one non-cationic lipids for the in vitro and/or in vivo delivery of biologically active molecules including nucleic acids, specifically DNA and RNA, and proteins into cells and tissues. Methods are also provided that use the compounds of the present invention to deliver biologically active molecules, into cells and tissues to facilitate the expression of target proteins therein. In some non-limiting embodiments, the subject lipid aggregate compositions can be used to deliver nucleic acid molecules to facilitate the expression of proteins involved in cellular reprogramming and genome editing applications.
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