公开(公告)号：US20130323837A1

公开(公告)日：2013-12-05

申请号：US13848587

申请日：2013-03-21

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： David JUDD ,  Paul Battista

IPC: C12N5/071

CPC classification number: C12N5/0629 ,  C12N2500/30 ,  C12N2500/32 ,  C12N2500/34 ,  C12N2500/35 ,  C12N2500/38 ,  C12N2500/42 ,  C12N2500/90 ,  C12N2501/01 ,  C12N2501/113

Abstract: The present invention provides cell culture media formulations which support the in vitro cultivation of animal epithelial cells. The media comprise at least one fibroblast growth factor (FGF) and at least one agent that induces increased intracellular cAMP levels, and optionally comprise ascorbic acid. The present invention also provides methods of cultivating animal epithelial cells in vitro using these cell culture media formulations, kits comprising the media, cell culture compositions comprising the culture media and an animal epithelial cell, and compositions that may be used as replacements for organ or gland extracts in animal cell culture media.

Abstract translation: 本发明提供支持体外培养动物上皮细胞的细胞培养基制剂。 培养基包含至少一种成纤维细胞生长因子（FGF）和至少一种诱导增加的细胞内cAMP水平并且任选地包含抗坏血酸的试剂。 本发明还提供了使用这些细胞培养基制剂在体外培养动物上皮细胞的方法，包含培养基的试剂盒，包含培养基和动物上皮细胞的细胞培养组合物，以及可用作器官或腺体的替代物的组合物 提取物在动物细胞培养基中。

公开(公告)号：US20140335616A1

公开(公告)日：2014-11-13

申请号：US13967247

申请日：2013-08-14

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： David JUDD ,  Paul Battista

IPC: C12N5/071

CPC classification number: C12N5/0629 ,  C12N2500/30 ,  C12N2500/32 ,  C12N2500/34 ,  C12N2500/35 ,  C12N2500/38 ,  C12N2500/42 ,  C12N2500/90 ,  C12N2501/01 ,  C12N2501/113

Abstract: The present invention provides cell culture media formulations which support the in vitro cultivation of animal epithelial cells. The media comprise at least one fibroblast growth factor (FGF) and at least one agent that induces increased intracellular cAMP levels, and optionally comprise ascorbic acid. The present invention also provides methods of cultivating animal epithelial cells in vitro using these cell culture media formulations, kits comprising the media, cell culture compositions comprising the culture media and an animal epithelial cell, and compositions that may be used as replacements for organ or gland extracts in animal cell culture media.

Abstract translation: 本发明提供支持体外培养动物上皮细胞的细胞培养基制剂。 培养基包含至少一种成纤维细胞生长因子（FGF）和至少一种诱导增加的细胞内cAMP水平并且任选地包含抗坏血酸的试剂。 本发明还提供了使用这些细胞培养基制剂在体外培养动物上皮细胞的方法，包含培养基的试剂盒，包含培养基和动物上皮细胞的细胞培养组合物，以及可用作器官或腺体的替代物的组合物 提取物在动物细胞培养基中。