中科微点-全球专利检索

  1. Login
  2. Sign Up

Search Results

4 records (took 0.014 seconds)

    METHODS AND REAGENTS FOR MOLECULAR CLONING
    1.
    发明申请
    METHODS AND REAGENTS FOR MOLECULAR CLONING 审中-公开
    分子克隆的方法和试剂

    公开(公告)号：US20150093788A1

    公开(公告)日：2015-04-02

    申请号：US14505396

    申请日：2014-10-02

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Jonathan CHESNUT ,  Stewart Shuman ,  Knut Madden ,  John Heyman ,  Robert Bennett

    IPC: C12N15/66 ,  C12N15/64

    CPC classification number: C12N15/66 ,  C12N15/10 ,  C12N15/64

    Abstract: The present invention provides compositions, methods, and kits for covalently linking nucleic acid molecules. The methods include a strand invasion step, and the compositions and kits are useful for performing such methods. For example, a method of covalently linking double stranded (ds) nucleic acid molecules can include contacting a first ds nucleic acid molecule, which has a topoisomerase linked to a 3′ terminus of one end and has a single stranded 5′ overhang at the same end, with a second ds nucleic acid molecule having a blunt end, such that the 5′ overhang can hybridize to a complementary sequence of the blunt end of the second nucleic acid molecule, and the topoisomerase can covalently link the ds nucleic acid molecules. The methods are simpler and more efficient than previous methods for covalently linking nucleic acid sequences, and the compositions and kits facilitate practicing the methods, including methods of directionally linking two or more ds nucleic acid molecules.

    Abstract translation: 本发明提供用于共价连接核酸分子的组合物，方法和试剂盒。 所述方法包括线入侵步骤，组合物和试剂盒对于进行这些方法是有用的。 例如，共价连接双链（ds）核酸分子的方法可以包括使第一个ds核酸分子接触，该第一个ds核酸分子具有与一个末端的3'末端连接的拓扑异构酶并且具有相同的单链（5'）突出端 末端，具有平末端的第二ds核酸分子，使得5'突出端可以与第二核酸分子的平端的互补序列杂交，并且拓扑异构酶可以共价连接ds核酸分子。 该方法比先前用于共价连接核酸序列的方法更简单和更有效，并且组合物和试剂盒有助于实施该方法，包括定向连接两个或多个ds核酸分子的方法。

Patent Agency Ranking