中科微点-全球专利检索

  1. Login
  2. Sign Up

Search Results

4 records (took 0.022 seconds)

    DETECTION AND QUANTIFICATION OF NUCLEIC ACID MOLECULES ASSOCIATED WITH A SURFACE
    2.
    发明申请
    DETECTION AND QUANTIFICATION OF NUCLEIC ACID MOLECULES ASSOCIATED WITH A SURFACE 审中-公开

    公开(公告)号：US20180363037A1

    公开(公告)日：2018-12-20

    申请号：US15781899

    申请日：2016-12-07

    Applicant: LIFE TECHNOLOGIES CORPORATION ,  THERMO FISHER SCIENTIFIC GENEART GMBH

    Inventor： Korbinian HEIL ,  Frank NOTKA ,  Kyle GEE ,  Jason DALLWIG ,  Phillip KUHN ,  Schuyler CORRY

    IPC: C12Q1/6834

    Abstract: This disclosure relates to methods, compositions, and related apparatuses for detection and quantification of nucleic acid molecules associated with a solid surface. Methods can include detecting or quantifying at least one nucleic acid molecule associated with a surface by contacting the at least one nucleic acid molecule with a fluorophore, wherein the fluorophore emits increased fluorescence at a wavelength when in contact with nucleic acid molecule, and detecting or measuring fluorescence from the fluorophore at the wavelength. Compositions can comprise a fluorophore and a nucleic acid molecule, wherein the nucleic acid molecule is associated with a surface, and the fluorophore has the property of emitting increased fluorescence at a wavelength when in contact with a nucleic acid molecule.

    Optically-Detectable Enzyme Substrates and Their Method of Use
    4.
    发明申请
    Optically-Detectable Enzyme Substrates and Their Method of Use 审中-公开
    光学检测酶底物及其使用方法

    公开(公告)号：US20160139135A1

    公开(公告)日：2016-05-19

    申请号：US14483392

    申请日：2014-09-11

    Applicant: Life Technologies Corporation

    Inventor： Schuyler CORRY ,  William Downey ,  Brian Filanoski ,  Kyle Gee ,  Lawrence Greenfield ,  James Hirsch ,  Iain Johnson ,  Aleksey Rukavishnikov

    IPC: G01N33/58 ,  C07D501/60

    CPC classification number: C07F5/022 ,  C07D499/00 ,  C07D501/00 ,  C07D501/58 ,  C07D501/60 ,  C07D503/00 ,  C07D519/00 ,  C12Q1/34 ,  C12Q2334/00 ,  G01N33/581 ,  G01N2333/986

    Abstract: The present invention relates to compounds that are substrates for an enzyme, and upon reaction with the enzyme provide a detectable response, such as an optically detectable response. In particular, the compounds have utility in detecting the presence of a β-lactamase in a sample. In addition to the compounds, methods are disclosed for analyzing a sample for the presence of a β-lactmase, for example, as an indicator of expression of a nucleic acid sequence including a sequence coding for a β-lactmase. Kits are disclosed that include the disclosed compounds and additional components, for example, cells, antibodies, a β-lactmase or instructions for using the components in an assay.

    Abstract translation: 本发明涉及作为酶的底物的化合物，并且在与酶反应时提供可检测的反应，例如可光学检测的反应。 特别地，这些化合物可用于检测样品中β-内酰胺酶的存在。 除了化合物之外，公开了用于分析样品存在β-乳糖酶的方法，例如，作为包括编码β-乳糖酶的序列的核酸序列的表达的指示剂。 公开了包括所公开的化合物和另外的组分的试剂盒，例如细胞，抗体，β-乳酸酶或在测定中使用组分的说明书。

Patent Agency Ranking