公开(公告)号：US20180362766A1

公开(公告)日：2018-12-20

申请号：US15955332

申请日：2018-04-17

Applicant: Life Technologies Corporation

Inventor： Kyle Gee ,  Upinder Singh ,  Aleksey Rukavishnikov ,  Daniel Beacham ,  Shih-Jung Huang ,  Michael Janes ,  Wenjun Zhou ,  Judith Berlier

IPC: C09B11/24 ,  G01N33/84 ,  C07D311/90 ,  A61K49/00 ,  G01N33/58 ,  G01N33/50

Abstract: Disclosed herein are compounds, compositions, methods and kits for detecting pH in samples using pH-sensitive fluorescent dyes. The compounds disclosed herein are novel xanthene-derivative dyes comprising an aniline moiety with one or more electron donating groups, which dyes are for detecting pH in samples either in vitro or in vivo. Also described herein are processes for preparing said dyes for use in the disclosed compositions, methods and kits.

公开(公告)号：US10648985B2

公开(公告)日：2020-05-12

申请号：US16107762

申请日：2018-08-21

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Kyle Gee ,  Upinder Singh ,  Scott Grecian ,  Scott Clarke

IPC: C07D213/46 ,  G01N33/58 ,  G01N33/533 ,  C07D213/74 ,  C07D213/79 ,  C07D213/81 ,  C07D215/12 ,  C07D401/04 ,  C07D235/14 ,  C07D213/53 ,  C07D213/68 ,  C07D213/78 ,  C09B23/06 ,  C09B23/08 ,  C09B11/24 ,  C07D401/14 ,  C07K14/575

Abstract: Low-copper click chemistry, 1.3-dipolar cycloadditions, and Staudinger ligations for modifying biomolecules is provided. Compositions, methods, and kits relating to low-copper click chemistry, 1.3-dipolar cycloadditions, and Staudinger ligations are also provided.

公开(公告)号：US10253185B2

公开(公告)日：2019-04-09

申请号：US15423890

申请日：2017-02-03

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Kyle Gee ,  Upinder Singh ,  Aleksey Rukavishnikov ,  Daniel Beacham ,  Shih-Jung Huang ,  Michael Janes ,  Wenjun Zhou

IPC: C09B11/24 ,  C07D311/90 ,  A61K49/00 ,  G01N33/58 ,  G01N33/50 ,  G01N33/84

Abstract: Disclosed herein are compounds, compositions, methods and kits for detecting pH in samples using pH-sensitive fluorescent dyes. The compounds disclosed herein are novel xanthene-derivative dyes comprising an aniline moiety with one or more electron donating groups, which dyes are for detecting pH in samples either in vitro or in vivo. Also described herein are processes for preparing said dyes for use in the disclosed compositions, methods and kits.

公开(公告)号：US09855287B2

公开(公告)日：2018-01-02

申请号：US14831220

申请日：2015-08-20

Applicant: Life Technologies Corporation ,  The Johns Hopkins University

Inventor： Brian Agnew ,  David Graham ,  Upinder Singh ,  Scott Grecian

IPC: A61K31/655 ,  A61K31/7024 ,  A61K45/06 ,  A61K31/7088 ,  A61K38/21

CPC classification number: A61K31/655 ,  A61K31/7024 ,  A61K31/7088 ,  A61K38/212 ,  A61K38/215 ,  A61K45/06 ,  A61K2300/00

Abstract: Methods of using azide-modified biomolecules, such as fatty acids, carbohydrates and lipids, to treat a plant, an insect or an animal infected with a virus or to inhibit infectivity of a virus, such as the human immunodeficiency virus, are provided. Also provided are methods of labeling a virus, such as human immunodeficiency virus, with an azide-modified biomolecule, such as a fatty acid, a carbohydrate, or an isoprenoid lipid. Also, provided are methods of tracking a virus in vivo, with an azide-modified biomolecule, such as a fatty acid, a carbohydrate, or an isoprenoid lipid. The azide-modified biomolecules may be combined with a pharmaceutically acceptable excipient to produce a pharmaceutical composition, optionally containing another anti-viral agent and/or a delivery agent, such as a liposome.

公开(公告)号：US11067580B2

公开(公告)日：2021-07-20

申请号：US16838004

申请日：2020-04-01

Applicant: Life Technologies Corporation

Inventor： Kyle Gee ,  Upinder Singh ,  Scott Grecian ,  Scott Clarke

IPC: C07D213/74 ,  G01N33/58 ,  G01N33/533 ,  C07D213/79 ,  C07D213/81 ,  C07D215/12 ,  C07D401/04 ,  C07D235/14 ,  C07D213/53 ,  C07D213/68 ,  C07D213/78 ,  C09B23/06 ,  C09B23/08 ,  C09B11/24 ,  C07D401/14 ,  C07K14/575

Abstract: Low-copper click chemistry, 1.3-dipolar cycloadditions, and Staudinger ligations for modifying biomolecules is provided. Compositions, methods, and kits relating to low-copper click chemistry, 1.3-dipolar cycloadditions, and Staudinger ligations are also provided.

公开(公告)号：US20180092932A1

公开(公告)日：2018-04-05

申请号：US15834639

申请日：2017-12-07

Applicant: Life Technologies Corporation ,  The Johns Hopkins University

Inventor： Brian Agnew ,  David Graham ,  Upinder Singh ,  Scott Grecian

IPC: A61K31/655 ,  A61K31/7024 ,  A61K45/06 ,  A61K38/21 ,  A61K31/7088

CPC classification number: A61K31/655 ,  A61K31/7024 ,  A61K31/7088 ,  A61K38/212 ,  A61K38/215 ,  A61K45/06 ,  A61K2300/00

Abstract: Methods of using azide-modified biomolecules, such as fatty acids, carbohydrates and lipids, to treat a plant, an insect or an animal infected with a virus or to inhibit infectivity of a virus, such as the human immunodeficiency virus, are provided. Also provided are methods of labeling a virus, such as human immunodeficiency virus, with an azide-modified biomolecule, such as a fatty acid, a carbohydrate, or an isoprenoid lipid. Also, provided are methods of tracking a virus in vivo, with an azide-modified biomolecule, such as a fatty acid, a carbohydrate, or an isoprenoid lipid. The azide-modified biomolecules may be combined with a pharmaceutically acceptable excipient to produce a pharmaceutical composition, optionally containing another anti-viral agent and/or a delivery agent, such as a liposome.

公开(公告)号：US20200333351A1

公开(公告)日：2020-10-22

申请号：US16838004

申请日：2020-04-01

Applicant: Life Technologies Corporation

Inventor： Kyle Gee ,  Upinder Singh ,  Scott Grecian ,  Scott Clarke

IPC: G01N33/58 ,  G01N33/533 ,  C07D213/74 ,  C07D213/79 ,  C07D213/81 ,  C07D215/12 ,  C07D401/04 ,  C07D235/14 ,  C07D213/53 ,  C07D213/68 ,  C07D213/78 ,  C09B23/06 ,  C09B23/08 ,  C09B11/24 ,  C07D401/14 ,  C07K14/575

Abstract: Low-copper click chemistry, 1.3-dipolar cycloadditions, and Staudinger ligations for modifying biomolecules is provided. Compositions, methods, and kits relating to low-copper click chemistry, 1.3-dipolar cycloadditions, and Staudinger ligations are also provided.

公开(公告)号：US20190105334A1

公开(公告)日：2019-04-11

申请号：US16208251

申请日：2018-12-03

Applicant: Life Technologies Corporation

Inventor： Brian Agnew ,  Upinder Singh ,  Scott Grecian

IPC: A61K31/655 ,  A61K31/7008 ,  C12N7/00

Abstract: Methods of using azide-modified biomolecules, such as fatty acids, carbohydrates and lipids, to treat a plant, an insect or an animal infected with a virus or to inhibit infectivity of a virus, such as the human immunodeficiency virus, are provided. Also provided are methods of labeling a virus, such as human immunodeficiency virus, with an azide-modified biomolecule, such as a fatty acid, a carbohydrate, or an isoprenoid lipid. Also, provided are methods of tracking a virus in vivo, with an azide-modified biomolecule, such as a fatty acid, a carbohydrate, or an isoprenoid lipid. The azide-modified biomolecules may be combined with a pharmaceutically acceptable excipient to produce a pharmaceutical composition, optionally containing another anti-viral agent and/or a delivery agent, such as a liposome.

公开(公告)号：US10179143B2

公开(公告)日：2019-01-15

申请号：US15671360

申请日：2017-08-08

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Brian Agnew ,  Upinder Singh ,  Scott Grecian

IPC: A61K31/655 ,  A61K31/7024 ,  A61K45/06 ,  A61K49/00 ,  A61K31/7088 ,  C12N7/00 ,  A61K31/7008

Abstract: Methods of using azide-modified biomolecules, such as fatty acids, carbohydrates and lipids, to treat a plant, an insect or an animal infected with a virus or to inhibit infectivity of a virus, such as the human immunodeficiency virus, are provided. Also provided are methods of labeling a virus, such as human immunodeficiency virus, with an azide-modified biomolecule, such as a fatty acid, a carbohydrate, or an isoprenoid lipid. Also, provided are methods of tracking a virus in vivo, with an azide-modified biomolecule, such as a fatty acid, a carbohydrate, or an isoprenoid lipid. The azide-modified biomolecules may be combined with a pharmaceutically acceptable excipient to produce a pharmaceutical composition, optionally containing another anti-viral agent and/or a delivery agent, such as a liposome.

公开(公告)号：US20150218379A1

公开(公告)日：2015-08-06

申请号：US14402358

申请日：2013-03-14

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Kyle Gee ,  Upinder Singh ,  Aleksey Rukavishnikov ,  Daniel Beacham ,  Shih-Jung Huang ,  Michael Janes ,  Wenjun Zhou

IPC: C09B11/24 ,  G01N33/58 ,  G01N33/50 ,  G01N33/84

CPC classification number: C09B11/24 ,  A61K49/0041 ,  C07D311/90 ,  G01N33/5005 ,  G01N33/582 ,  G01N33/84

Abstract: Disclosed herein are compounds, compositions, methods and kits for detecting pH in samples using pH-sensitive fluorescent dyes. The compounds disclosed herein are novel xanthene-derivative dyes comprising an aniline moiety with one or more electron donating groups, which dyes are for detecting pH in samples either in vitro or in vivo. Also described herein are processes for preparing said dyes for use in the disclosed compositions, methods and kits.

Abstract translation: 本文公开了使用pH敏感荧光染料检测样品中的pH的化合物，组合物，方法和试剂盒。 本文公开的化合物是包含具有一个或多个给电子基团的苯胺部分的新型呫吨衍生物染料，该染料用于在体外或体内检测样品中的pH。 本文还描述了用于制备用于所公开的组合物，方法和试剂盒的所述染料的方法。