公开(公告)号：US20250109412A1

公开(公告)日：2025-04-03

申请号：US18911619

申请日：2024-10-10

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Xavier De Mollerat Du Jeu

IPC: C12N15/88 ,  A61K47/54 ,  A61K47/64 ,  C07K14/00

Abstract: The present invention is directed to non-naturally occurring peptides containing a membrane-penetrating amino acid sequence and further at least one polycationic moiety or peptide sequence. The peptides are suitable for use in delivery a cargo to the interior of a cell. Suitable cargo includes nucleic acid molecules (including DNA, RNA or PNA), polypeptides, or other biologically active molecules. The present invention is further directed to transfection complexes containing the non-naturally occurring peptides of the present invention in non-covalent association with at least one cationic lipid and a cargo to be delivered to the interior of a cell. The invention further relates to methods for the preparation and use of the non-naturally occurring peptides for the formation of transfection complexes and the delivery of a cargo to the interior of a cell in culture, an animal or a human. The invention also relates to compositions and kits useful for transfecting cells.

公开(公告)号：US20250129320A1

公开(公告)日：2025-04-24

申请号：US18689952

申请日：2022-10-07

Applicant: Life Technologies Corporation

Inventor： Xavier De Mollerat Du Jeu

IPC: C12M1/00 ,  C12M1/06 ,  C12M1/36 ,  C12N15/86

Abstract: Methods and systems for improving the scalability and associated efficacy of continuous transfection processes, such as continuous transient transfection processes. The continuous transfection system includes at least a first input line and a second input line, wherein the first input line and the second input line are configured for sterile, fluid connection to a first reagent housing and a second reagent housing, respectively, a mixing chamber that is fluidly connected to the at least first and second input lines, a delivery line fluidly connected to the mixing chamber, wherein the delivery line is configured for sterile, fluid connection to a cell culture vessel, and a flow rate control component, such as a pump operable to control flow rate of liquid into the at least first and second input lines, throughout the mixing chamber, and into the cell culture vessel.

公开(公告)号：US20240263124A1

公开(公告)日：2024-08-08

申请号：US18624847

申请日：2024-04-02

Applicant: LIFE TECHNOLOGIES CORPORATION ,  LIFE TECHNOLOGIES HOLDINGS PTE LTD

Inventor： Steven Huei Yeo ,  Xavier De Mollerat Du Jeu ,  Way Xuang Lee ,  Nektaria Andronikou ,  Benyong Shi ,  Kok Shyong Chong ,  Han Wei ,  Siu Wee Hon ,  Yvonne Peck ,  Chee Wai Chan ,  Beng Heng Lim ,  Stacey Mei Lin Teo ,  Dhanaraj Shanmugam ,  Jun Yan Tham ,  Michael Gordon ,  Chris Rossman ,  Ulrich Forke ,  Li Yang Lim

IPC: C12M1/42 ,  C12M1/00 ,  C12M1/06 ,  C12M1/34 ,  C12M1/36 ,  C12M3/00

CPC classification number: C12M35/02 ,  C12M23/14 ,  C12M23/44 ,  C12M27/02 ,  C12M29/00 ,  C12M41/12 ,  C12M41/48

Abstract: The present disclosure provides electroporation cartridges for single use electroporation as well as electroporation cartridges for automated batch processing, electroporation instruments and systems and methods of electroporation using these devices and systems. In some embodiments, electroporation cartridges comprise an electroporation chamber defined by an elongate body, a first electrode at a proximal end and a second electrode at a distal end of a chamber. Electroporation systems of the disclosure include one or more components including a pulse generator, compartments for placing either flow-through or single use electroporation cartridges, components for storage of cells, cooling and pre-cooling mechanisms, removably insertable modular casings having compartments for holding and arranging electroporation system and reagent components, one or more pumps for moving sample through the system, and processors and controllers.