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公开(公告)号：US12125299B2
公开(公告)日：2024-10-22
申请号：US17755610
申请日：2020-11-04
发明人： Jelle Van Der Horst
CPC分类号： G06V20/695 , G06T7/0016 , G06T7/70 , G06V10/751 , G06T2207/10056
摘要： Methods and systems for determining interaction between cells are described wherein the method includes determining or receiving a sequence of images representing manipulating first cells, in a holding space, the holding space including a functionalized wall comprising second cells, the manipulating including settling of the first cells onto the functionalized wall and applying a force on the settled first cells; detecting groups of pixels representing first cells in first images representing the settling of the first cells onto the functionalized wall; tracking locations of detected first cells in the first images; and, determining settling events, a settling event being determined if a cell in a first image is not distinguishable from background of the first image, the location in the image at which a cell settling event is detected defining a cell settling location; detecting groups of pixels representing cells in second images captured during the application of the force and tracking locations of detected cells, wherein tracked locations of a detected cell in the second images form a tracking path, the first location of the tracking path defining a pop-up event, the location in a second image at which a pop-up event is detected defining a pop-up location; and, determining detachment events based on the settling locations and based on the pop-up locations, a detachment event defining a first cell being detached from a second cell due to application of the force on the first cell, and determining information about the interaction between first and second cells based on the force applied to the first cells.
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公开(公告)号：US20220366708A1
公开(公告)日：2022-11-17
申请号：US17755610
申请日：2020-11-04
发明人： Jelle Van Der Horst
摘要： Methods and systems for determining interaction between cells are described wherein the method includes determining or receiving a sequence of images representing manipulating first cells, in a holding space, the holding space including a functionalized wall comprising second cells, the manipulating including settling of the first cells onto the functionalized wall and applying a force on the settled first cells; detecting groups of pixels representing first cells in first images representing the settling of the first cells onto the functionalized wall; tracking locations of detected first cells in the first images; and, determining settling events, a settling event being determined if a cell in a first image is not distinguishable from background of the first image, the location in the image at which a cell settling event is detected defining a cell settling location; detecting groups of pixels representing cells in second images captured during the application of the force and tracking locations of detected cells, wherein tracked locations of a detected cell in the second images form a tracking path, the first location of the tracking path defining a pop-up event, the location in a second image at which a pop-up event is detected defining a pop-up location; and, determining detachment events based on the settling locations and based on the pop-up locations, a detachment event defining a first cell being detached from a second cell due to application of the force on the first cell, and determining information about the interaction between first and second cells based on the force applied to the first cells.
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公开(公告)号：US20240011883A1
公开(公告)日：2024-01-11
申请号：US18027789
申请日：2021-09-23
发明人： Jelle Van Der Horst
IPC分类号： G01N15/00
CPC分类号： G01N15/00 , G01N2015/0003 , G01N2015/0065 , G01N2015/1006
摘要： A method for determining interaction between cellular bodies and a functionalized wall surface comprises: obtaining a sequence of images representing manipulating cellular bodies in a holding space having a functionalized wall surface configured to bind the cellular bodies that includes applying a force; tracking locations of pixel groups in respective images out of the sequence of images, each pixel group in the images representing a cellular body out of the cellular bodies, the locations in the respective images defining a trajectory of the cellular body moving relative to the functionalized wall surface; determining one or more speed values of the cellular body at one or more locations of the trajectory, the one or more speed values being higher than zero; and, classifying that the cellular body is attached to the functionalized wall surface based on the one or more speed values and at least one threshold speed value.
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