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公开(公告)号：US12125299B2
公开(公告)日：2024-10-22
申请号：US17755610
申请日：2020-11-04
发明人： Jelle Van Der Horst
CPC分类号： G06V20/695 , G06T7/0016 , G06T7/70 , G06V10/751 , G06T2207/10056
摘要： Methods and systems for determining interaction between cells are described wherein the method includes determining or receiving a sequence of images representing manipulating first cells, in a holding space, the holding space including a functionalized wall comprising second cells, the manipulating including settling of the first cells onto the functionalized wall and applying a force on the settled first cells; detecting groups of pixels representing first cells in first images representing the settling of the first cells onto the functionalized wall; tracking locations of detected first cells in the first images; and, determining settling events, a settling event being determined if a cell in a first image is not distinguishable from background of the first image, the location in the image at which a cell settling event is detected defining a cell settling location; detecting groups of pixels representing cells in second images captured during the application of the force and tracking locations of detected cells, wherein tracked locations of a detected cell in the second images form a tracking path, the first location of the tracking path defining a pop-up event, the location in a second image at which a pop-up event is detected defining a pop-up location; and, determining detachment events based on the settling locations and based on the pop-up locations, a detachment event defining a first cell being detached from a second cell due to application of the force on the first cell, and determining information about the interaction between first and second cells based on the force applied to the first cells.
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公开(公告)号：US20240316550A1
公开(公告)日：2024-09-26
申请号：US18574549
申请日：2022-06-30
CPC分类号： B01L3/50273 , G01H1/00 , B01L2200/0647 , B01L2300/0654 , B01L2400/0436
摘要： A method is disclosed for characterizing an acoustic-based particle manipulation device comprising a sample holder having a holding space containing a fluid medium. The holding space comprises a wall surface portion. The method comprises providing a driving signal to the sample holder for generating an acoustic wave in the holding space that drives a particle at the wall surface portion, away from the wall surface portion. The method further comprises measuring vibrations of at least part of a surface of the sample holder caused by said driving signal. The method also comprises, based on the measured vibrations of said at least part of the surface, determining, for each position out of a plurality of positions at the wall surface portion and in the holding space, a value for an acoustic force that is caused by said driving signal and that acts in a direction away from the wall surface portion.
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公开(公告)号：US20230324341A1
公开(公告)日：2023-10-12
申请号：US18003244
申请日：2021-07-12
IPC分类号： G01N29/036 , G01N29/22
CPC分类号： G01N29/036 , G01N29/222 , G01N2291/014 , G01N2291/02466
摘要： The disclosure relates to methods and systems for manipulating a particle, in particular for studying small particles and/or biological cellular bodies. A disclosed method comprises the steps of: providing a sample holder (3) comprising a holding space (5) for holding a sample comprising a particle (9) in a fluid medium (11); providing a driving signal, having a signal frequency, a signal amplitude and a signal power, to the sample holder generating an acoustic wave in the sample holder (3) for applying an acoustic force to the particle (9), the wave having a frequency; determining an acoustic resonance frequency of the sample holder and determining a quality factor for the sample holder with respect to the resonance frequency. An associated system (1) is also disclosed.
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公开(公告)号：US20230314297A1
公开(公告)日：2023-10-05
申请号：US18023292
申请日：2021-09-01
IPC分类号： G01N15/14
CPC分类号： G01N15/14 , G01N2015/149
摘要： A method of sorting cellular bodies comprises receiving images representing manipulation of first cellular bodies in a holding space of a flow cell including or connected to a sorting device, the manipulation including: allowing some of the first cellular bodies to contact a functionalized wall of the holding space; applying a force to the contacted first cellular bodies for detaching some of the first cellular bodies; and, transporting the cellular bodies to the sorting device; and, processing the sequence of images of the first cellular bodies and controlling the sorting device based on the image processing by detecting detachments of first cellular bodies in the images; determining for each detected detached first cell a detachment force; tracking the location of detached first cellular bodies during the transport of the cellular bodies to the sorting device; and, sorting the cellular bodies by controlling the sorting device based on the detachment force.
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公开(公告)号：US20220026291A1
公开(公告)日：2022-01-27
申请号：US17497338
申请日：2021-10-08
发明人： Graeme Alan KING , Andreas Sebastian BIEBRICHER , Iddo HELLER , Erwin Johannes Gerard PETERMAN , Gijs Jan Lodewijk WUITE
摘要： One aspect of this disclosure relates to a computer-implemented method for determining a force acting on at least part of a structure, for example a biological structure, such as a DNA molecule. The method comprises controlling a light-sensitive system, e.g. of a microscope, to determine light information based on light from the structure. The light is incident on at least a part of the light sensitive system. The light-sensitive system may be said to capture the light from the structure. The at least part of the structure comprises one or more optically active entities, such as DNA intercalator molecules and donor/acceptor fluorophores. At least one of (i) an optical activity of the entities and (ii) a quantity of the entities depends on the force acting on the at least part of the structure. Furthermore, the light information defines a light property value associated with said at least part of the structure. The method further comprises determining the force acting on the at least part of the structure on the basis of said light property value and a reference light property value.
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公开(公告)号：US12053778B2
公开(公告)日：2024-08-06
申请号：US17529498
申请日：2021-11-18
发明人： Andrea Candelli , Felix Oswald , Gerrit Sitters
IPC分类号： B01L3/00 , B01L7/00 , G01N15/1429 , G01N21/03 , G01N21/17 , G01N29/036 , G01N29/22 , G01N29/24 , G01N29/34 , G01N21/64
CPC分类号： B01L3/502761 , B01L3/50273 , B01L7/00 , G01N15/1429 , G01N21/0332 , G01N29/036 , G01N29/222 , G01N29/2437 , G01N29/2462 , G01N29/346 , G01N29/348 , B01L2200/0652 , B01L2300/0663 , B01L2300/163 , B01L2300/1822 , B01L2400/0436 , G01N2021/177 , G01N21/6458 , G01N2291/014 , G01N2291/02466 , G01N2291/02475 , G01N2291/0255 , G01N2291/0256 , G01N2291/0257 , G01N2291/02809 , G01N2291/0423 , G01N2291/0426
摘要： A method of manipulating and/or investigating cellular bodies (9) is provided. The method comprises the steps of: providing a sample holder (3) comprising a holding space (5) for holding a fluid medium (11); providing a sample (7) comprising one or more cellular bodies (9) in a fluid medium (11) in the holding space (5); generating an acoustic wave in the holding space exerting a force (F) on the sample (7) in the holding space (5). The method further comprises providing the holding space (5) with a functionalised wall surface portion (17) to be contacted by the sample (7) and the sample (7) is in contact with the functionalised wall surface portion (17) during at least part of the step of application of the acoustic wave. A system and a sample holder (3) are also provided.
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公开(公告)号：US20240319075A1
公开(公告)日：2024-09-26
申请号：US18574553
申请日：2022-07-01
CPC分类号： G01N21/1702 , G01N15/14 , G01N2015/1006 , G01N2021/1706
摘要： A method of manipulating objects is provided which comprises providing a sample holder comprising a holding space for holding an at least partly fluid sample; providing a sample comprising one or more of the objects in a fluid medium in the holding space; controllably causing a local variation of one or more condition for the objects in at least part of the holding space; and removing at least a fraction of the objects from the holding space. The method also comprises the further steps of determining a spatial dependency of the local variation, and phototagging on the basis of the determined spatial dependency at least part of the objects in the holding space in one or more tagging positions thus providing tagged objects, wherein the step of removing at least a fraction of the objects from the holding space comprises removing at least part of the tagged objects.
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8.发明公开公开(公告)号：US20240240998A1
公开(公告)日：2024-07-18
申请号：US18574542
申请日：2022-06-27
发明人： Douwe Kamsma , Felix Oswald
CPC分类号： G01K15/005 , B01L3/502761 , G01K11/26 , G05D23/1951 , B01L2200/0663 , B01L2300/0663 , B01L2400/0436
摘要： A method is disclosed for measuring a temperature of a fluid in a holding space of an acoustic-based particle manipulation device. The method comprises performing a calibration. The calibration comprises measuring, using a reference temperature sensor, the temperature of the fluid to be a reference temperature value. The calibration also comprises determining a reference resonance frequency of the acoustic-based particle manipulation device having the fluid in its holding space at the reference temperature value. The method further comprises measuring the temperature of the fluid comprising determining a resonance frequency of the acoustic-based particle manipulation device and determining, based on the reference resonance frequency determined during the calibration and based on the determined resonance frequency, the temperature of the fluid to be a temperature value.
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公开(公告)号：US20230372931A1
公开(公告)日：2023-11-23
申请号：US18030170
申请日：2021-10-07
发明人： Myles McGarrigle
IPC分类号： B01L3/00
CPC分类号： B01L3/502723 , B01L2200/0684 , B01L2300/028 , B01L2400/0436 , B01L2400/0478
摘要： A method of reducing or preventing bubbles in a microfluidic sample liquid is provided. The method comprises providing a microfluidic sample holder comprising an enclosed fluid channel for holding at least part of the sample liquid, and filling at least part of the fluid channel with a sample liquid. The method further comprises: a step of pressurizing the sample liquid in the fluid channel to raise a sample liquid pressure to an elevated pressure higher than an ambient pressure and an operating pressure, a step of maintaining the sample liquid pressure at least at the elevated pressure for a predetermined period to cause dissolving of gas into the sample liquid, and a step of reducing the sample liquid pressure from the elevated pressure to the operating pressure. An associated system is also provided.
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公开(公告)号：US20220366708A1
公开(公告)日：2022-11-17
申请号：US17755610
申请日：2020-11-04
发明人： Jelle Van Der Horst
摘要： Methods and systems for determining interaction between cells are described wherein the method includes determining or receiving a sequence of images representing manipulating first cells, in a holding space, the holding space including a functionalized wall comprising second cells, the manipulating including settling of the first cells onto the functionalized wall and applying a force on the settled first cells; detecting groups of pixels representing first cells in first images representing the settling of the first cells onto the functionalized wall; tracking locations of detected first cells in the first images; and, determining settling events, a settling event being determined if a cell in a first image is not distinguishable from background of the first image, the location in the image at which a cell settling event is detected defining a cell settling location; detecting groups of pixels representing cells in second images captured during the application of the force and tracking locations of detected cells, wherein tracked locations of a detected cell in the second images form a tracking path, the first location of the tracking path defining a pop-up event, the location in a second image at which a pop-up event is detected defining a pop-up location; and, determining detachment events based on the settling locations and based on the pop-up locations, a detachment event defining a first cell being detached from a second cell due to application of the force on the first cell, and determining information about the interaction between first and second cells based on the force applied to the first cells.
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