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公开(公告)号：US20100313995A1
公开(公告)日：2010-12-16
申请号：US12794330
申请日：2010-06-04
IPC分类号： B67C3/26
CPC分类号： G01N35/0099 , B01L2200/027 , G01N2035/00158
摘要： A robot is described for automatically performing microfluidic experiments that are often complex and repetitive. The robot includes a system for initiating the communication of off-chip fluids, such as fluorocarbons, and off-chip materials, such as proteins. The communication of off-chip fluids and off-chip materials is further guided to a microfluidics device vis-à-vis a Z-head robot, an XY plane robot, and an interface assembly that creates a fluid-tight joint for communicating the off-chip fluids and off-chip materials into the microfluidics device for conduction of microfluidics experiments.
摘要翻译： 描述了一种自动执行微流体实验的机器人，这些实验往往是复杂和重复的。 机器人包括用于启动片外流体（例如碳氟化合物）和片外材料（例如蛋白质）的通信的系统。 片外流体和片外材料的通信被进一步引导到微流体装置相对于Z头机器人，XY平面机器人和接口组件，其形成用于传递关闭的流体密封接头 将芯片流体和片外材料转入微流体装置中用于微流体实验的传导。
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公开(公告)号：US06818060B2
公开(公告)日：2004-11-16
申请号：US10184037
申请日：2002-06-26
IPC分类号： C30B714
CPC分类号： C30B7/00 , C30B29/58 , Y10S117/90 , Y10T117/10 , Y10T117/1004
摘要： A matrix mixing robot includes a plurality of precision pumps (such as precision syringe pumps), a distributor and a processor system. Each pump, under the control of the processor system, draws an associated stock solution from a stock solution source, and pumps the drawn stock solution out through an outlet. The distributor, also under the control of the processor system, directs a stock solution from a particular pump outlet to a selected solution receptacle. A multi-port distribution valve may be associated with each precision pump. Each valve, under control of the processor system, can connect its associated pump to one the pump's inlets or outlets.
摘要翻译： 矩阵混合机器人包括多个精密泵（例如精密注射泵），分配器和处理器系统。 处理器系统控制下的每个泵从原液解决方案中抽出一个相关的库存解决方案，并通过一个出口将抽出的库存溶液抽出来。 分配器也在处理器系统的控制下，将库存溶液从特定的泵出口引导到选定的溶液容器。 多口分配阀可以与每个精密泵相关联。 处理器系统控制下的每个阀都可以将其相关的泵连接到泵的入口或出口。
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公开(公告)号：US20100158772A1
公开(公告)日：2010-06-24
申请号：US12484983
申请日：2009-06-15
CPC分类号： B01D9/0072 , B01D9/00 , Y10T428/31855
摘要： A nanovolume microcapillary crystallization system allows nanoliter-volume screening of crystallization conditions in a crystal card that allows crystals to either be removed for traditional cryoprotection or in situ X-ray diffraction studies on protein crystals that grow within. The system integrates formulation of crystallization cocktails with preparation of the crystallization experiments. The system allows the researcher to select either gradient screening in crystallization experiments for efficient exploration of crystallization phase space or a combination of sparse matrix with gradient screening to execute one comprehensive hybrid crystallization trial.
摘要翻译： 纳米级微毛细管结晶系统允许纳米级体积筛选晶体卡中的结晶条件，其允许将晶体除去用于传统的冷冻保护或在其内生长的蛋白质晶体的原位X射线衍射研究。 该系统将结晶鸡尾酒配方与结晶实验的准备相结合。 该系统允许研究者选择结晶实验中的梯度筛选，以有效探索结晶相空间，或者通过梯度筛选稀释基质的组合进行一次综合混合结晶试验。
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公开(公告)号：US06267935B1
公开(公告)日：2001-07-31
申请号：US09344007
申请日：1999-06-25
IPC分类号： B09D900
CPC分类号： B01L3/06 , B01L3/5085 , C30B7/00 , C30B29/58
摘要： In one aspect, the present invention provides crystallization solutions useful for crystallizing proteins and other molecules, especially macromolecules. In a presently preferred embodiment, the crystallization solutions of the invention are combined as four crystallization solution sets. Each of the four crystallization solution sets includes forty eight different crystallization solutions. Each individual crystallization solution includes a precipitant and a buffer, and optionally includes at least one additive. In another aspect, the present invention provides kits including a plurality of crystallization solutions of the present invention and at least one crystallization plate that preferably includes a plurality of reservoirs. Preferably the crystallization solutions are disposed within the reservoirs of the crystallization plates.
摘要翻译： 一方面，本发明提供了用于结晶蛋白质和其它分子，特别是大分子的结晶溶液。 在目前优选的实施方案中，将本发明的结晶溶液组合为四个结晶溶液组。 四个结晶溶液组中的每一个包括四十八种不同的结晶溶液。 每个单独的结晶溶液包括沉淀剂和缓冲剂，并且任选地包括至少一种添加剂。 在另一方面，本发明提供了包括本发明的多种结晶溶液和至少一种优选包括多个储存器的结晶板的试剂盒。 优选地，结晶溶液设置在结晶板的储存器内。
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公开(公告)号：US07587284B2
公开(公告)日：2009-09-08
申请号：US11338861
申请日：2006-01-24
IPC分类号： G01N33/50
摘要： Pieces of software and databases are used to facilitate the design and synthesis of genes. The synthesis techniques allow identification, quantification, transcription, translation, and manipulation of portions of the gene sequence represented as 0's and 1's in a computing system. These pieces of software and databases compile constraint information provided by a user to create synthetic genes to express proteins lacking disordered or variable regions and containing surface mutations that promote solubility and/or crystallization. Regions of the gene sequence that do not form stable structures can be removed and replaced by a short biological linker to improve crystallization of proteins.
摘要翻译： 使用软件和数据库来促进基因的设计和合成。 合成技术允许在计算系统中识别，定量，转录，翻译和操纵表示为0和1的基因序列的部分。 这些软件和数据库编译由用户提供的约束信息，以创建合成基因以表达缺乏无序或可变区的蛋白质并且含有促进溶解度和/或结晶的表面突变。 不能形成稳定结构的基因序列的区域可以被短的生物接头取代并代替，以改善蛋白质的结晶。
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公开(公告)号：US06950757B2
公开(公告)日：2005-09-27
申请号：US10121094
申请日：2002-04-11
申请人： Lansing J. Stewart
发明人： Lansing J. Stewart
IPC分类号： B01D9/02 , C12Q1/68 , G01N31/00 , G01N33/48 , G01N33/50 , G01N33/53 , G01N33/543 , G01N33/68 , G06F19/00
CPC分类号： G01N33/54313 , G01N33/68
摘要： This invention relates to crystallization based assays for identifying ligands that bind to a macromolecule.
摘要翻译： 本发明涉及用于鉴定结合大分子的配体的基于结晶的测定法。
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公开(公告)号：US06811608B1
公开(公告)日：2004-11-02
申请号：US09631185
申请日：2000-08-02
IPC分类号： C30B714
摘要： Disclosed is a system and a method for depositing crystallization observations into a relational database. The system includes a computer, microscope, and a headset having a microphone and headphones. Crystallization observations are deposited into the database by verbal commands. Verbal commands also drive the crystallization apparatus plates to position the apparatus wells within the microscope field of vision. The system is integrated with a software application which enables the user to build the trials, matrices, solutions, and macromolecules to be used in the crystallization trials. The user is guided through the crystallization trial setup by a multitude of managers and builders of the software application. The software application also provides numerous tools to query the database and display results in a multitude of formats.
摘要翻译： 公开了一种用于将结晶观察结果存入关系数据库的系统和方法。 该系统包括具有麦克风和耳机的计算机，显微镜和耳机。 结晶观察通过语言命令存入数据库。 语言命令还驱动结晶装置板将装置孔定位在显微镜视野内。 该系统与软件应用程序集成，使用户能够构建用于结晶试验的试验，矩阵，解决方案和大分子。 用户由软件应用程序的众多经理和构建者引导通过结晶试验设置。 软件应用程序还提供了许多工具来查询数据库并以多种格式显示结果。
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