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    Manipulation of fluids and reactions in microfluidic systems
    4.
    发明授权
    Manipulation of fluids and reactions in microfluidic systems 有权
    在微流体系统中操纵流体和反应

    公开(公告)号：US08772046B2

    公开(公告)日：2014-07-08

    申请号：US12525749

    申请日：2008-02-06

    Applicant: Seth Fraden ,  Galder Cristobal-Azkarate

    Inventor： Seth Fraden ,  Galder Cristobal-Azkarate

    IPC: G01N1/38 ,  B01L3/00

    CPC classification number: B01L3/502784 ,  B01L3/06 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502746 ,  B01L7/54 ,  B01L2200/0621 ,  B01L2200/0642 ,  B01L2200/0668 ,  B01L2200/0673 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/1822 ,  B01L2400/0406 ,  B01L2400/0655 ,  C30B7/00 ,  C30B29/58 ,  G01N1/38 ,  G01N2001/386 ,  Y10T137/0318 ,  Y10T436/2575

    Abstract: Microfluidic structures and methods for manipulating fluids and reactions are provided. Such structures and methods may involve positioning fluid samples, e.g., in the form of droplets, in a carrier fluid (e.g., an oil, which may be immiscible with the fluid sample) in predetermined regions in a microfluidic network. In some embodiments, positioning of the droplets can take place in the order in which they are introduced into the microfluidic network (e.g., sequentially) without significant physical contact between the droplets. Because of the little or no contact between the droplets, there may be little or no coalescence between the droplets. Accordingly, in some such embodiments, surfactants are not required in either the fluid sample or the carrier fluid to prevent coalescence of the droplets. Structures and methods described herein also enable droplets to be removed sequentially from the predetermined regions.

    Abstract translation: 提供微流体结构和操纵流体和反应的方法。 这样的结构和方法可以涉及在微流体网络中的预定区域中将例如液​​滴形式的流体样品定位在载体流体（例如，可能与流体样品不混溶的油）中。 在一些实施例中，液滴的定位可以按照它们被引入微流体网络（例如，顺序地）而没有液滴之间的显着物理接触的顺序进行。 由于液滴之间很少或没有接触，所以液滴之间可能很少或没有聚结。 因此，在一些这样的实施方案中，在流体样品或载体流体中不需要表面活性剂以防止液滴的聚结。 本文所述的结构和方法还使得能够从预定区域顺序地去除液滴。

    Hanging Droplet Plate
    5.
    发明申请
    Hanging Droplet Plate 有权
    挂水滴板

    公开(公告)号：US20140106395A1

    公开(公告)日：2014-04-17

    申请号：US14002269

    申请日：2012-03-02

    Applicant: Christof Fattinger ,  Patrick Iaiza ,  Tom Kissling ,  Dieter Voegelin ,  Thomas Zumstein ,  Claudia Mcginnis

    Inventor： Christof Fattinger ,  Patrick Iaiza ,  Tom Kissling ,  Dieter Voegelin ,  Thomas Zumstein ,  Claudia Mcginnis

    IPC: G01N33/50

    CPC classification number: G01N33/5014 ,  B01L3/06 ,  B01L3/50853 ,  B01L3/5088 ,  B01L2300/042 ,  B01L2300/047 ,  B01L2300/0829 ,  C12M23/12 ,  C12M25/01

    Abstract: A hanging droplet plate (1) comprises a predetermined number of droplet compartments (10) each being capable of receiving a droplet of a liquid. The respective droplet compartment (10) comprises a circumferential microfluidic wetting barrier (102) which is arranged to surround a respective cavity (100) and which prevents a droplet from spreading beyond the microfluidic wetting barrier (102). The respective compartment (10) comprises a closed bottom (101) and at least one additional circumferential microfluidic wetting barrier (104), each additional circumferential microfluidic wetting barrier (104) which is arranged to surround a preceding circumferential microfluidic wetting barrier (102). A wettable area (103) is arranged between two adjacently arranged microfluidic wetting barriers (102, 104).

    Abstract translation: 悬挂的液滴板（1）包括预定数量的液滴隔室（10），每个液滴隔室（10）能够接收液滴。 相应的液滴隔室（10）包括周向微流体润湿屏障（102），其被布置成围绕相应的空腔（100）并且防止液滴扩散到微流体润湿屏障（102）之外。 相应的隔室（10）包括封闭的底部（101）和至少一个附加的周向微流体润湿屏障（104），每个附加的周向微流体润湿屏障（104）被布置成围绕先前的周向微流体润湿屏障（102）。 可湿性区域（103）布置在两个相邻布置的微流体润湿屏障（102,104）之间。

    METHOD OF LOADING A CRYSTALLIZATION DEVICE
    7.
    发明申请
    METHOD OF LOADING A CRYSTALLIZATION DEVICE 有权
    装载结晶装置的方法

    公开(公告)号：US20110269941A1

    公开(公告)日：2011-11-03

    申请号：US13121359

    申请日：2009-09-28

    Applicant: Johann Kubicek ,  Frank Schaefer ,  Joerg Labahn ,  Georg Bueldt

    Inventor： Johann Kubicek ,  Frank Schaefer ,  Joerg Labahn ,  Georg Bueldt

    IPC: C07K14/00 ,  B65B1/20 ,  B01D9/02

    CPC classification number: C07K1/306 ,  B01D9/00 ,  B01D9/0077 ,  B01D2009/0086 ,  B01L3/06 ,  C07K14/705

    Abstract: The present invention pertains to a method for loading a crystallization device and for manufacturing a crystallization device comprising multiple receptacles with a pre-defined amount of at least one matrix-forming compound capable of forming a crystallization matrix for a membrane protein, said method comprising the following steps: a) Modifying the state of aggregation of said at least one matrix-forming compound to a fluidic state which allows dispensing said at least one matrix-forming compound, and b) dispensing a defined amount of said at least one matrix-forming compound into at least one receptacle of the crystallization device, wherein said dispensed matrix-forming compound solidifies within said receptacle. Thereby, pre-filled crystallization devices are obtained which can be used as consumables in particular in automated crystallization processes. Also provided are protein crystallization methods using respectively prepared crystallization devices.

    Abstract translation: 本发明涉及一种用于装载结晶装置和用于制造结晶装置的方法，所述结晶装置包括具有预定量的至少一种能够形成膜蛋白的结晶基质的基质形成化合物的多个容器，所述方法包括 以下步骤：a）将所述至少一种基质形成化合物的聚集状态改变为允许分配所述至少一种基质形成化合物的流体状态，以及b）分配限定量的所述至少一种基质形成 化合物进入结晶装置的至少一个容器中，其中所述分配的基质形成化合物在所述容器内固化。 由此，可以获得预填充的结晶装置，其可以用作特别是在自动结晶过程中的消耗品。 还提供了使用分别制备的结晶装置的蛋白质结晶方法。

    METHODS AND DEVICES FOR CRYSTALLIZATION BY CONTROLLED EVAPORATION
    9.
    发明申请
    METHODS AND DEVICES FOR CRYSTALLIZATION BY CONTROLLED EVAPORATION 有权
    通过控制蒸发进行结晶的方法和装置

    公开(公告)号：US20100314051A1

    公开(公告)日：2010-12-16

    申请号：US12483787

    申请日：2009-06-12

    Applicant: Jack Barger ,  James Sinclair ,  Michael P. Emery

    Inventor： Jack Barger ,  James Sinclair ,  Michael P. Emery

    IPC: B01D1/12 ,  B01D1/00

    CPC classification number: B01D3/346 ,  B01D1/14 ,  B01L3/06 ,  B01L3/50853 ,  B01L2200/0678 ,  B01L2300/048 ,  B01L2300/049 ,  B01L2300/0829 ,  G01N2001/4027

    Abstract: Methods and devices for controlled evaporation of solvent from a solution are provided. In one embodiment, the device can control evaporation of different solutions comprising solvents of dissimilar volatilities simultaneously. In another embodiment, control over the flow of solvent vapor out of the device is provided by selecting the pressure at an inlet port and an exhaust port of the device. In yet another embodiment, control over the flow of solvent vapor out of the device is provided by selecting the size of a disk flow control orifice positioned between the wells and a vapor removal outlet of the device. In still another embodiment, control over the flow of solvent vapor out of the device is provided by the opening and closing of the vapor removal outlet of the device. In yet a further embodiment, the rate of evaporation of solvent is controlled by controlling the temperature of the solution. The flow rate control mechanisms can be used alone or in any combination to control evaporation of one or more solvents from solutions.

    Abstract translation: 提供了从溶液中控制溶剂蒸发的方法和装置。 在一个实施方案中，该装置可以同时控制包含不同挥发性溶剂的不同溶液的蒸发。 在另一个实施例中，通过选择装置的入口和排气口处的压力来提供通过装置流出的溶剂蒸气的控制。 在另一个实施方案中，通过选择位于孔之间的盘流量控制孔和装置的蒸汽除去出口的尺寸来提供从装置出来的溶剂蒸气流的控制。 在另一个实施方案中，通过打开和关闭装置的蒸汽除去出口来提供从装置出来的溶剂蒸气流的控制。 在又一个实施方案中，通过控制溶液的温度来控制溶剂的蒸发速率。 流量控制机构可以单独使用或以任何组合使用以控制溶液中的一种或多种溶剂的蒸发。

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