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    Porous acrylonitrile polymer substrate for bonding ligands and separating biologically active substances
    1.
    发明授权
    Porous acrylonitrile polymer substrate for bonding ligands and separating biologically active substances 失效
    用于结合配体和分离生物活性物质的多孔丙烯腈聚合物底物

    公开(公告)号：US5306632A

    公开(公告)日：1994-04-26

    申请号：US22223

    申请日：1993-02-25

    申请人： Larry S. Anderson ,  Michael T. Cooke ,  David A. Ley

    发明人： Larry S. Anderson ,  Michael T. Cooke ,  David A. Ley

    IPC分类号： B01J20/285 ,  B01D15/08 ,  B01D71/42 ,  B01J20/281 ,  C08F8/00 ,  C08J9/22 ,  D01D5/24 ,  D01F6/18 ,  G01N30/88 ,  C12N11/08 ,  C12N11/06 ,  G01N33/546 ,  G01N33/549

    CPC分类号： C08F8/00 ,  Y10S435/915 ,  Y10S436/824

    摘要： A porous shaped substrate such as a porous bead is formed from polyacrylonitrile or a copolymer thereof containing nitrile groups. The substrate has a hydrophilic surface containing amide groups constituting about 1.8 mole percent to less than about 15 mole percent of the total nitrile groups, and containing no amide or carboxyl groups. The substrate is substantially non-swellable in water and is able to resist pressures in a columnar bed of up to about 3000 psi without collapsing. In forming the amide groups, polyacrylonitrile or copolymer thereof containing nitrile groups, an alkaline catalyst such as sodium hydroxide and a nonsolvent for the substrate such as methanol are combined to form a suspension. A peroxide is added to the suspension and the suspension is heated to hydrolyze nitrile groups to amide groups. Succinylated aminoethyl groups or activated carboxyl groups can be formed on the substrate and a bioactive ligand such as p-aminobenzamidine covalently bonded to the substrate. The bound ligand can then be used to complex a biologically active substance such as an enzyme to separate the substance from solution.

    摘要翻译： 由聚丙烯腈或其含有腈基的共聚物形成多孔形状的基材如多孔珠粒。 底物具有含有酰胺基团的亲水性表面，其构成总腈基团的约1.8摩尔％至小于约15摩尔％，并且不含酰胺基或羧基。 基材在水中基本上不溶胀，并且能够抵抗高达约3000psi的柱状床中的压力而不会塌陷。 在形成酰胺基团时，将含有腈基的聚丙烯腈或其共聚物，氢氧化钠等碱性催化剂和甲醇等底物的非溶剂合并形成悬浮液。 向悬浮液中加入过氧化物，加热悬浮液将腈基水解成酰胺基。 可以在底物上形成琥珀酰氨基乙基或活化的羧基，以及与基底共价结合的生物活性配体如对氨基苯甲脒。 然后可以将结合的配体用于复合生物活性物质如酶，以将物质与溶液分离。