公开(公告)号：US20100169449A1

公开(公告)日：2010-07-01

申请号：US12721977

申请日：2010-03-11

申请人： Lin Lin ,  Ping LIN

发明人： Lin Lin ,  Ping LIN

IPC分类号： G06F15/16

CPC分类号： H04M3/533 ,  H04M7/006

摘要： A method and apparatus for accessing messages from a message server. Messages are stored on the message server in their current format without transcoding. When a client opens a session with the message server, a data transfer rate is determined for the client. In response to a message retrieve request, the bit rate of the message is compared to the data transfer rate, and if less than the data transfer rate, the message is downloaded to the user terminal without transcoding. If the bit rate is greater than the data transfer rate, then the message is transcoded to a lower bit rate according to a codec supported by the user. The codec may be selected from a set of codecs supported by the user terminal. The user is provided with the capability to override the system selected codec, for example, to pick a codec for secure encoding or encryption.

摘要翻译： 一种用于从消息服务器访问消息的方法和装置。 消息以当前格式存储在消息服务器上，无需转码。 当客户端与消息服务器打开会话时，为客户端确定数据传输速率。 响应于消息检索请求，将消息的比特率与数据传输速率进行比较，如果小于数据传输速率，则将消息下载到用户终端而不进行转码。 如果比特率大于数据传输速率，则根据用户支持的编解码器将消息转码为较低的比特率。 可以从用户终端支持的一组编解码器中选择编解码器。 为用户提供覆盖系统选择的编解码器的能力，例如选择用于安全编码或加密的编解码器。

公开(公告)号：US20100057468A1

公开(公告)日：2010-03-04

申请号：US12200384

申请日：2008-08-28

申请人： Lin LIN ,  Ping LIN

发明人： Lin LIN ,  Ping LIN

IPC分类号： G10L21/00

CPC分类号： G06F3/167 ,  G06F17/00 ,  G06F17/2247 ,  G10L21/00 ,  G10L2015/228

摘要： A method, system and voice browser execute voice applications to perform a voice-based function. A document is retrieved and parsed to create a parse tree. Script code is created from the parse tree, thereby consuming part of the parse tree to create a reduced parse tree. The reduced parse tree is stored in a cache for subsequent execution to perform the voice-based function.

摘要翻译： 一种方法，系统和语音浏览器执行语音应用来执行基于语音的功能。 检索并解析文档以创建解析树。 脚本代码是从解析树创建的，从而消耗部分解析树来创建一个简化的分析树。 缩减的分析树被存储在高速缓存中以便随后执行以执行基于语音的功能。

公开(公告)号：US20080206757A1

公开(公告)日：2008-08-28

申请号：US11841972

申请日：2007-08-20

申请人： Ping LIN ,  Andrea Ghetti ,  Wenge Shi ,  Mengjia Tang ,  Gioulnar I. Harvie ,  Huimin Tao ,  Guoliang Tao ,  Lei Wu ,  David Cerny ,  Jia Xu

发明人： Ping LIN ,  Andrea Ghetti ,  Wenge Shi ,  Mengjia Tang ,  Gioulnar I. Harvie ,  Huimin Tao ,  Guoliang Tao ,  Lei Wu ,  David Cerny ,  Jia Xu

IPC分类号： C12Q1/58 ,  C12N1/20 ,  G01N33/53

CPC分类号： C12N5/0693 ,  G01N33/5094 ,  G01N33/54346 ,  G01N33/56972 ,  G01N33/574 ,  G01N2015/1006 ,  G01N2015/1402 ,  G01N2333/70525 ,  G01N2333/70589 ,  G01N2800/52 ,  Y10T436/25375

摘要： The present invention provides methods and compositions for isolating and detecting rare cells from a biological sample containing other types of cells. In particular, the present invention includes a debulking step that uses a microfabricated filters for filtering fluid samples and the enriched rare cells can be used in a downstream process such as identifies, characterizes or even grown in culture or used in other ways. The invention also include a method of determining the aggressiveness of the tumor or of the number or proportion of cancer cells in the enriched sample by detecting the presence or amount of telomerase activity or telomerase nucleic acid or telomerase expression after enrichment of rare cells. This invention further provides an efficient and rapid method to specifically remove red blood cells as well as white blood cells from a biological sample containing at least one of each of red blood cells and white blood cells, resulting in the enrichment of rare target cells including circulating tumor cells (CTC), stromal cells, mesenchymal cells, endothelial cells, fetal cells, stem cells, non-hematopoietic cells etc from a blood sample. The method is based upon combination of immuno-microparticles (antibody coated microparticles) and density-based separation. The final enriched target cells can be subjected to a variety of analysis and manipulations, such as flowcytometry, PCR, immunofluorescence, immunocytochemistry, image analysis, enzymatic assays, gene expression profiling analysis, efficacy tests of therapeutics, culturing of enriched rare cells, and therapeutic use of enriched rare cells. In addition, depleted plasma protein and white blood cells can be optionally recovered, and subjected to other analysis such as inflammation studies, gene expression profiling, etc.

摘要翻译： 本发明提供从含有其他类型细胞的生物样品中分离和检测稀有细胞的方法和组合物。 特别地，本发明包括减压步骤，其使用微制造过滤器来过滤流体样品，并且富集的稀有细胞可以用于下游过程，例如识别，表征甚至在培养中生长或以其它方式使用。 本发明还包括通过在稀释细胞富集后检测端粒酶活性或端粒酶核酸或端粒酶表达的存在或数量来确定肿瘤侵袭性或富集样品中癌细胞数量或比例的方法。 本发明还提供了一种从含有红细胞和白细胞中的至少一种的生物样品中特异性除去红细胞以及白细胞的有效快速方法，导致稀有靶细胞的富集，包括循环 来自血液样品的肿瘤细胞（CTC），基质细胞，间充质细胞，内皮细胞，胎儿细胞，干细胞，非造血细胞等。 该方法基于免疫微粒（抗体包被的微粒）和基于密度的分离的组合。 最终的富集的靶细胞可以进行各种分析和操作，例如流式细胞术，PCR，免疫荧光，免疫细胞化学，图像分析，酶测定，基因表达谱分析，治疗剂的功效测试，培养富集的稀有细胞和治疗 使用富集的稀有细胞。 此外，可以任选地回收贫化的血浆蛋白和白细胞，并进行其他分析，例如炎症研究，基因表达谱等。