公开(公告)号：US07829311B2

公开(公告)日：2010-11-09

申请号：US12172385

申请日：2008-07-14

申请人： Linda Jane DeCarolis ,  Stephen R. Fahnestock

发明人： Linda Jane DeCarolis ,  Stephen R. Fahnestock

IPC分类号： C12P21/04 ,  C12N9/90 ,  C12N15/00 ,  C12N1/20 ,  C07K14/00

CPC分类号： C12P21/02 ,  C07K2319/50 ,  C12N9/90

摘要： An acid-resistant peptide solubility tag (an “inclusion body tag”) is provided that is effective in producing peptides of interest in an insoluble form. Fusion peptide constructs comprising the inclusion body tag fused to a peptide of interest are provided. An acid cleavable peptide moiety separates the inclusion body tag from the peptide of interest so that acid hydrolysis can be used during subsequent processing steps to separate the tag from the desired peptide of interest. The present inclusion body tag's resistance to acid hydrolysis facilitates easier and cleaner separation of the peptide of interest after acid hydrolysis. Specifically, a ketosteroid isomerase-derived inclusion body tag is provided that has been engineered to be more resistant to acid hydrolysis.

摘要翻译： 提供了一种耐酸肽溶解性标签（“包含体标签”），其有效地生产不溶形式的感兴趣的肽。 提供了包含与感兴趣的肽融合的包含体标签的融合肽构建体。 可酸切割肽部分将包涵体标签与感兴趣的肽分开，使得在随后的加工步骤中可以使用酸水解，以将标签与期望的目标肽分离。 本发明的包涵体标签对酸水解的抗性促进了酸水解后感兴趣的肽的更容易和更清洁的分离。 具体地说，提供了一种经过工程改造以耐酸解作用的酮类异构酶衍生的包含体标签。