公开(公告)号：US5130232A

公开(公告)日：1992-07-14

申请号：US536860

申请日：1990-06-12

申请人： Lucy F. Lee ,  Zhizhong Cui ,  Richard L. Witter

发明人： Lucy F. Lee ,  Zhizhong Cui ,  Richard L. Witter

IPC分类号： C07K16/10

CPC分类号： C07K16/1036 ,  Y10S435/973 ,  Y10S436/811

摘要： The combination of monoclonal antibodies 11A25 and 11E32, which recognize two distinct antigenic sites on the 62-kd glycoprotein of reticuloendotheliosis virus (REV) in ELISA, enables an increase in the sensitivity of an ELISA assay for subtype 2 REV over what was heretofore possible. The combination of MAbs is useful for diagnosing REV antigen and antibody in poultry flocks and for detecting new strains and variants of the viral subtype.

摘要翻译： 在ELISA中识别网状内皮组织增生病毒（REV）的62-kd糖蛋白上的两个不同抗原位点的单克隆抗体11A25和11E32的组合使得能够增加针对亚型2 REV的ELISA测定的灵敏度比迄今为止可能的更高。 MAb的组合可用于诊断家禽群中的REV抗原和抗体，并用于检测病毒亚型的新菌株和变体。

公开(公告)号：US06322780B1

公开(公告)日：2001-11-27

申请号：US08709731

申请日：1996-09-09

申请人： Lucy F. Lee ,  Keyvan Nazerian ,  Richard L. Witter ,  Ping Wu ,  Noboru Yanagida ,  Shigeto Yoshida

发明人： Lucy F. Lee ,  Keyvan Nazerian ,  Richard L. Witter ,  Ping Wu ,  Noboru Yanagida ,  Shigeto Yoshida

IPC分类号： A61K4800

CPC分类号： C07K14/005 ,  A61K39/00 ,  C07K2319/00 ,  C12N2710/16322

摘要： A nucleotide sequence encoding the gp82 polypeptide of Marek's disease virus is disclosed. Also disclosed are recombinant viruses which are useful as vaccines for protecting against Marek's Disease, preferably containing two or more genes encoding Marek's Disease Virus antigens such as glycoprotein B and glycoprotein gp82, under the control of a poxvirus promoter within a region of the DNA of fowlpox virus which is not essential for virus growth. Also provided is a vaccine exhibiting a synergistic immunoprotective effect, comprising a recombinant fowlpox virus expressing Marek's Disease Virus gB protein in combination with turkey herpesvirus. A method of immunizing poultry, comprising administering any of the disclosed vaccines, is also provided.

摘要翻译： 公开了编码马立克氏病病毒的gp82多肽的核苷酸序列。 还公开了重组病毒，其可用作保护抗马立克氏病的疫苗，优选含有两种或更多种编码马立克氏病病毒抗原的基因，例如糖蛋白B和糖蛋白gp82，在禽痘DNA的区域内的痘病毒启动子控制下 病毒不是病毒生长所必需的。 还提供了表现出协同免疫保护作用的疫苗，其包含与火鸡疱疹病毒组合的表达马立克氏病病毒gB蛋白的重组禽痘病毒。 还提供了免疫家禽的方法，包括施用任何所公开的疫苗。

公开(公告)号：US5849299A

公开(公告)日：1998-12-15

申请号：US723037

申请日：1991-06-28

申请人： Richard L. Witter

发明人： Richard L. Witter

IPC分类号： A61K39/255 ,  A61K39/00 ,  A61P31/12 ,  C07K14/055

CPC分类号： C07K14/005 ,  A61K39/00 ,  C12N2710/16322

摘要： A Marek's disease vaccine comprising an attenuated revertant virus derived as a clone of Md11/75/R2 or an antigenic component of the virus is characterized by a markedly reduced pathogenicity compared to the parent virus, without a comparable loss in immunogenicity. Attenuated revertants of the invention are exemplified by Md11/75/R2/23 and Md11/75/R2/29. These viruses or immunogenic components thereof can be formulated into monovalent and polyvalent vaccines.

摘要翻译： 包含衍生自Md11 / 75 / R2或该病毒的抗原成分的克隆的减毒回复病毒的马立克氏病疫苗的特征在于与亲本病毒相比显着降低的致病性，而免疫原性没有相当的损失。 本发明的衰减回复体由Md11 / 75 / R2 / 23和Md11 / 75 / R2 / 29举例说明。 这些病毒或其免疫原性成分可以配制成单价和多价疫苗。

公开(公告)号：US5403582A

公开(公告)日：1995-04-04

申请号：US7282

申请日：1993-01-21

申请人： Keyvan Nazerian ,  Jay G. Calvert ,  Richard L. Witter ,  Noboru Yanagida

发明人： Keyvan Nazerian ,  Jay G. Calvert ,  Richard L. Witter ,  Noboru Yanagida

IPC分类号： A61K39/00 ,  C07K14/15 ,  C12N15/48 ,  C12N15/863 ,  A61K39/21

CPC分类号： C12N15/86 ,  C07K14/005 ,  A61K39/00 ,  C12N2710/24022 ,  C12N2740/11022

摘要： A recombinant fowlpox virus is provided which is useful as a vaccine for protection against avian reticuloendo-theliosis virus-associated diseases. In a specific embodiment, the recombinant virus expresses a gene encoding an envelope glycoprotein of spleen necrosis virus under the control of a poxvirus promoter, inserted in a nonessential region of the fowlpox virus genome.

摘要翻译： 提供重组禽痘病毒，其可用作用于防止禽网状神经胶质瘤病毒相关疾病的疫苗。 在具体实施方案中，重组病毒在插入在禽痘病毒基因组的非必需区域中的痘病毒启动子的控制下，表达编码脾坏死病毒的包膜糖蛋白的基因。

公开(公告)号：US4895718A

公开(公告)日：1990-01-23

申请号：US71949

申请日：1987-07-10

申请人： Richard L. Witter

发明人： Richard L. Witter

IPC分类号： A61K39/255

CPC分类号： A61K39/255 ,  A61K39/12 ,  A61K2039/5254 ,  A61K2039/552 ,  C12N2710/16334 ,  Y10S424/816 ,  Y10S435/948

摘要： A serotype 2 Marek's disease vaccine comprising a cloned strain designated as 301B/1 has been derived from a field isolate. This strain is characterized by superior levels of replicative ability and protectivity in chickens as compared to existing commercial serotype 2 strains, and it is virtually nonpathogenic. 301B/1 is particularly useful in the formulation of highly efficacious bivalent and polyvalent vaccines.

摘要翻译： 包含称为301B / 1的克隆菌株的血清型2马立克氏病疫苗已经从野外分离物得到。 与现有的商业血清型2株相比，该菌株的特征在于鸡的复制能力和保护力水平优异，并且几乎不致致病性。 301B / 1在制备高效二价和多价疫苗中特别有用。

公开(公告)号：US5565202A

公开(公告)日：1996-10-15

申请号：US289818

申请日：1994-08-12

申请人： Richard L. Witter

发明人： Richard L. Witter

IPC分类号： A61K39/255 ,  A61K39/12

CPC分类号： A61K39/255 ,  A61K39/12 ,  A61K2039/5254 ,  A61K2039/552 ,  C12N2710/16334 ,  Y10S424/816

摘要： A vaccine for Marek's disease using mutant strains of a serotype 2 field isolate, designated 471B/1, is disclosed. The mutant viruses, which are produced by serial passage of 471B/1 in cell culture, not only retain effective immunizing ability against Marek's disease virus, but which also exhibit reduced enhancement of lymphoid leukosis in comparison to non-passaged 471B/1 and conventional serotype 2 vaccines. These viruses or immunogenic components thereof can be formulated into monovalent and polyvalent vaccines.

摘要翻译： 公开了使用名称为471B / 1的血清型2场分离株的突变株的马立克氏病疫苗。 通过在细胞培养物中连续传代471B / 1突变病毒，不仅保留了针对马立克氏病病毒的有效免疫能力，而且与未传代的471B / 1和常规血清型 2种疫苗。 这些病毒或其免疫原性成分可以配制成单价和多价疫苗。

公开(公告)号：US4895717A

公开(公告)日：1990-01-23

申请号：US071948

申请日：1987-07-10

申请人： Richard L. Witter

发明人： Richard L. Witter

IPC分类号： A61K39/255

CPC分类号： A61K39/255 ,  A61K39/12 ,  A61K2039/5254 ,  A61K2039/552 ,  C12N2710/16334 ,  Y10S424/816 ,  Y10S435/948

摘要： A Marek's disease vaccine comprising either a revertant virus derived by backpassaging an attenuated serotype 1 Md11 virus or an antigenic component of the virus is characterized by increased levels of replicative ability and protectivity in chickens as compared to the original attenuated strain. Revertants of the invention are exemplified by a clone identified as Md11/75C/R2 and can be formulated into monovalent and polyvalent vaccines.

摘要翻译： 一种马立克氏病疫苗，其包含通过逆转减毒血清型Md11病毒或病毒的抗原成分衍生的回复病毒，其特征在于与原始减毒菌株相比，鸡的复制能力和保护性水平提高。 本发明的回复体的例证是鉴定为Md11 / 75C / R2的克隆，并且可以配制成单价和多价疫苗。