公开(公告)号：US5786182A

公开(公告)日：1998-07-28

申请号：US850207

申请日：1997-05-02

申请人： Luigi Catanzariti ,  Bryan W. Kluttz ,  Geoff A. McKinley ,  Arthur L. Garland ,  Louis Graziano ,  James G. Moe ,  Marcela Vera-Garcia ,  James Clement Bishop ,  David Chastain ,  Fabio Gennari

发明人： Luigi Catanzariti ,  Bryan W. Kluttz ,  Geoff A. McKinley ,  Arthur L. Garland ,  Louis Graziano ,  James G. Moe ,  Marcela Vera-Garcia ,  James Clement Bishop ,  David Chastain ,  Fabio Gennari

IPC分类号： B01L3/00 ,  B01L7/00 ,  C12P19/34 ,  C12M3/00 ,  C12N15/00 ,  G01N21/00

CPC分类号： B01L3/502 ,  B01L7/52 ,  B01L2200/026 ,  B01L2300/0825 ,  B01L2300/0861 ,  B01L2400/049 ,  B01L2400/0655

摘要： A reaction vessel for a nucleic acid amplification reaction has a first chamber containing an amplification reagent mix, a second chamber containing an amplification enzyme, and a fluid channel or chamber connecting the first and second chambers together. A fluid sample is introduced into the first chamber. After a denaturation and primer annealing process has occurred in the first chamber, the fluid channel is opened to allow the solution of the reagent and fluid sample to flow into the second chamber. The second chamber is maintained at an optimal temperature for the amplification reaction. A station is described for processing test strips incorporating the reaction vessels. The station includes temperature and vacuum control subsystems to maintain proper temperatures in the reaction vessel and effectuate the transfer of the fluid from one chamber to the other in an automated fashion without human intervention.

摘要翻译： 用于核酸扩增反应的反应容器具有包含扩增试剂混合物的第一室，含有扩增酶的第二室和将第一和第二室连接在一起的流体通道或室。 将流体样品引入第一室。 在第一室中发生变性和引物退火过程之后，打开流体通道以允许试剂和流体样品的溶液流入第二室。 第二个室保持在扩增反应的最佳温度。 描述了一个工位，用于处理加入反应容器的试片。 该站包括温度和真空控制子系统，以在反应容器中保持适当的温度，并且以无需人为干预的方式实现流体从一个室到另一个室的转移。

公开(公告)号：US6103503A

公开(公告)日：2000-08-15

申请号：US107352

申请日：1998-06-30

申请人： Bryan W. Kluttz ,  Arthur L. Garland ,  Geoff A. McKinley ,  Luigi Catanzariti

发明人： Bryan W. Kluttz ,  Arthur L. Garland ,  Geoff A. McKinley ,  Luigi Catanzariti

IPC分类号： B01L3/00 ,  B01L7/00 ,  C12Q1/68 ,  C12P19/34

CPC分类号： C12Q1/6848 ,  B01L3/5023 ,  B01L7/52 ,  B01L2300/069 ,  B01L2400/0406 ,  B01L2400/0481 ,  B01L3/5029

摘要： A testing apparatus is described that contains a test sample and one or more reagents, or reaction solutions associated with chemical reactions and a resilient, compressible, porous material, and which is amenable to subsequent process or reaction steps or liquid transfer steps. A piece of sponge or foam rubber, which is compatible with the amplification reactions and amplification products, is introduced into the reaction vessel and absorbs the test sample and reagents, or reaction solutions associated with an amplification process, or amplification products thus, reducing their loss through aerosolation.

摘要翻译： 描述了一种测试装置，其包含测试样品和一种或多种试剂，或与化学反应相关的反应溶液和弹性的可压缩的多孔材料，并且其适用于随后的工艺或反应步骤或液体转移步骤。 将与扩增反应和扩增产物相容的一块海绵或泡沫橡胶引入反应​​容器中并吸收与扩增过程相关的测试样品和试剂或反应溶液，或扩增产物，从而减少其损失 通过气溶胶。