公开(公告)号：US20050240013A1

公开(公告)日：2005-10-27

申请号：US10518980

申请日：2003-06-18

申请人： Luigi De Ambrosi ,  Nicola Iannaccone ,  Sergio Gonella ,  Elena Vismara ,  Solitario Nesti ,  Giangiaocomo Torri

发明人： Luigi De Ambrosi ,  Nicola Iannaccone ,  Sergio Gonella ,  Elena Vismara ,  Solitario Nesti ,  Giangiaocomo Torri

IPC分类号： C08B37/00 ,  C07B31/00 ,  C08B37/10

CPC分类号： C08B37/0063 ,  C08B37/0075 ,  C08B37/0078

摘要： The invention relates to a process for the depolymerization of glycosaminoglycanes characterized by the use of electron beam radiation, optionally in the presence of an organic compound selected from the group consisting of ethers, alcohols, aldehydes, amides and formic acid. The invention also relates to the intermediate depolymerized heparin obtained by the process. The intermediate depolymerized heparin can be dissolved in a buffer solution and fractionated by Gel Permeation for obtaining the desired Molecular Weight.

摘要翻译： 本发明涉及通过使用电子束辐射特征的糖胺聚糖解聚方法，任选地在选自醚，醇，醛，酰胺和甲酸的有机化合物存在下进行。 本发明还涉及通过该方法得到的中间解聚肝素。 中间解聚的肝素可以溶解在缓冲溶液中，并通过凝胶渗透分级，以获得所需的分子量。

公开(公告)号：US07321034B2

公开(公告)日：2008-01-22

申请号：US10518980

申请日：2003-06-18

申请人： Luigi De Ambrosi ,  Nicola Iannaccone ,  Sergio Gonella ,  Elena Vismara ,  Solitario Nesti ,  Giangiaocomo Torri

发明人： Luigi De Ambrosi ,  Nicola Iannaccone ,  Sergio Gonella ,  Elena Vismara ,  Solitario Nesti ,  Giangiaocomo Torri

IPC分类号： C08B37/10

CPC分类号： C08B37/0063 ,  C08B37/0075 ,  C08B37/0078

摘要： The invention relates to a process for the depolymerization of glycosaminoglycanes characterized by the use of electron beam radiation, optionally in the presence of an organic compound selected from the group consisting of ethers, alcohols, aldehydes, amides and formic acid. The invention also relates to the intermediate depolymerized heparin obtained by the process. The intermediate depolymerized heparin can be dissolved in a buffer solution and fractionated by gel permeation for obtaining the desired molecular weight.

摘要翻译： 本发明涉及通过使用电子束辐射特征的糖胺聚糖解聚方法，任选地在选自醚，醇，醛，酰胺和甲酸的有机化合物存在下进行。 本发明还涉及通过该方法得到的中间解聚肝素。 中间解聚的肝素可以溶解在缓冲溶液中并通过凝胶渗透分馏以获得所需的分子量。

公开(公告)号：US07939512B2

公开(公告)日：2011-05-10

申请号：US11279699

申请日：2006-04-13

申请人： Luigi De Ambrosi ,  Sergio Gonella ,  Donata Bensi ,  Giangiacomo Torri ,  Antonella Bisio ,  Elena Vismara

发明人： Luigi De Ambrosi ,  Sergio Gonella ,  Donata Bensi ,  Giangiacomo Torri ,  Antonella Bisio ,  Elena Vismara

IPC分类号： A61K31/727 ,  C08B37/10

CPC分类号： C08B37/0078

摘要： The present invention is directed to a multistep process for the physical depolymerization of heparin wherein heparin is subjected to at least two γ-ray irradiations and wherein between two irradiation steps the depolymerised heparin is subjected to a separation step and only a fraction of the depolymerised heparin obtained from the first irradiation is subjected to the second irradiation step. It is also directed to heparin-derived oligosaccharide fractions obtainable by the process of the invention.

摘要翻译： 本发明涉及用于肝素物理解聚的多步骤方法，其中肝素进行至少两次γ射线照射，并且其中在两个照射步骤之间进行解聚的肝素进行分离步骤，并且仅将解聚的肝素的一部分 从第一次照射得到的第二照射工序。 还涉及通过本发明的方法获得的肝素衍生的寡糖级分。

公开(公告)号：US20070082869A1

公开(公告)日：2007-04-12

申请号：US11279699

申请日：2006-04-13

申请人： Luigi De Ambrosi ,  Sergio Gonella ,  Donata Bensi ,  Giangiacomo Torri ,  Antonella Bisio ,  Elena Vismara

发明人： Luigi De Ambrosi ,  Sergio Gonella ,  Donata Bensi ,  Giangiacomo Torri ,  Antonella Bisio ,  Elena Vismara

IPC分类号： A61K31/727 ,  C08B37/10

CPC分类号： C08B37/0078

摘要： The present invention is directed to a multistep process for the physical depolymerization of heparin wherein heparin is subjected to at least two γ-ray irradiations and wherein between two irradiation steps the depolymerised heparin is subjected to a separation step and only a fraction of the depolymerised heparin obtained from the first irradiation is subjected to the second irradiation step. It is also directed to heparin-derived oligosaccharide fractions obtainable by the process of the invention.

摘要翻译： 本发明涉及用于肝素物理解聚的多步法，其中肝素经受至少两次伽马射线照射，并且其中在两个照射步骤之间进行解聚的肝素进行分离步骤，并且仅将解聚的肝素的一部分 从第一次照射得到的第二照射工序。 还涉及通过本发明的方法获得的肝素衍生的寡糖级分。

公开(公告)号：US07091337B2

公开(公告)日：2006-08-15

申请号：US10477293

申请日：2003-03-11

申请人： Luigi De Ambrosi ,  Sergio Gonella ,  Elena Vismara

发明人： Luigi De Ambrosi ,  Sergio Gonella ,  Elena Vismara

IPC分类号： C08B37/10

CPC分类号： C08B37/0063 ,  C08B37/0069 ,  C08B37/0072 ,  C08B37/0075 ,  C08B37/0078

摘要： The invention relates to a process for the depolymerization of glycosaminoglycanes characterized by the use of high-energy radiation in the presence of an organic compound selected from the group consisting of ethers, alcohols, aldehydes, amides and formic acid. The invention also relates to intermediate depolymerized heparin having a molecular weight of between 1,000 and 5,500, absorbance at 400 nm below 0.300 and ratio SO3−/COO−equal to or higher than in the starting heparin. The intermediate depolymerized heparin can be dissolved in a buffer solution and fractionated by gel permeation for obtaining the desired molecular weight.

摘要翻译： 本发明涉及一种用于解聚糖胺聚糖的方法，其特征在于在选自醚，醇，醛，酰胺和甲酸的有机化合物存在下使用高能辐射。 本发明还涉及分子量为1,000至5,500的中间解聚肝素，400nm处的吸光度低于0.300，SO 3的比例< / SUP>等于或高于起始肝素。 中间解聚的肝素可以溶解在缓冲溶液中并通过凝胶渗透分馏以获得所需的分子量。