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公开(公告)号：US20200325465A1
公开(公告)日：2020-10-15
申请号：US16864098
申请日：2020-04-30
发明人： Alexandra Merkx-Jacques , David Woodhall , Mark Scaife , Roberto E. Armenta , Denise Muise , Holly Rasmussen , Jeremy Benjamin
摘要： Provided herein are recombinant microorganisms having two or more copies of a nucleic acid sequence encoding xylose isomerase, wherein the nucleic acid encoding the xylose isomerase is an exogenous nucleic acid. Optionally, the recombinant microorganisms include at least one nucleic acid sequence encoding a xylulose kinase and/or at least one nucleic acid sequence encoding a xylose transporter. The provided recombinant microorganisms are capable of growing on xylose as a carbon source.
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公开(公告)号：US20210388312A1
公开(公告)日：2021-12-16
申请号：US17286449
申请日：2019-10-21
发明人： Kimberly Hyson , Nathalia Florez , Denise Muise , Alexandra Merkx-Jacques , Dorothy Dennis , David Woodhall , Zachary Sun , Roberto E. Armenta
摘要： Provided herein are methods of making microorganisms modified for increased xylose consumption as compared to unmodified microorganisms. The methods include providing xylose-consuming microorganisms comprising two or more copies of a nucleic acid sequence encoding xylose isomerase and two or more copies of a nucleic acid sequence encoding a xylose kinase, culturing the microorganisms in medium containing xylose and harvesting a portion of the microorganisms. These steps are repeated multiple times. The microorganisms are then isolated. The isolated microorganisms have increased xylose consumption rates compared to control xylose-consuming microorganisms. Also provided are a population of microorganisms made by the provided methods. Methods of culturing the population of microorganisms and methods of reducing xylitol production in cultures comprising the population of microorganisms are provided.
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公开(公告)号：US10662418B2
公开(公告)日：2020-05-26
申请号：US15918786
申请日：2018-03-12
发明人： Alexandra Merkx-Jacques , David Woodhall , Mark Scaife , Roberto E. Armenta , Denise Muise , Holly Rasmussen , Jeremy Benjamin
摘要： Provided herein are recombinant microorganisms having two or more copies of a nucleic acid sequence encoding xylose isomerase, wherein the nucleic acid encoding the xylose isomerase is an exogenous nucleic acid. Optionally, the recombinant microorganisms include at least one nucleic acid sequence encoding a xylulose kinase and/or at least one nucleic acid sequence encoding a xylose transporter. The provided recombinant microorganisms are capable of growing on xylose as a carbon source.
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公开(公告)号：US20180291363A1
公开(公告)日：2018-10-11
申请号：US15918786
申请日：2018-03-12
发明人： Alexandra Merkx-Jacques , David Woodhall , Mark Scaife , Roberto E. Armenta , Denise Muise , Holly Rasmussen , Jeremy Benjamin
摘要： Provided herein are recombinant microorganisms having two or more copies of a nucleic acid sequence encoding xylose isomerase, wherein the nucleic acid encoding the xylose isomerase is an exogenous nucleic acid. Optionally, the recombinant microorganisms include at least one nucleic acid sequence encoding a xylulose kinase and/or at least one nucleic acid sequence encoding a xylose transporter. The provided recombinant microorganisms are capable of growing on xylose as a carbon source.
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公开(公告)号：US09951326B2
公开(公告)日：2018-04-24
申请号：US15208849
申请日：2016-07-13
发明人： Alexandra Merkx-Jacques , David Woodhall , Mark Scaife , Roberto E. Armenta , Denise Muise , Holly Rasmussen , Jeremy Benjamin
CPC分类号： C12N9/92 , C07K14/40 , C07K2319/21 , C12N9/1205 , C12Y207/01017 , C12Y503/01005
摘要： Provided herein are recombinant microorganisms having two or more copies of a nucleic acid sequence encoding xylose isomerase, wherein the nucleic acid encoding the xylose isomerase is an exogenous nucleic acid. Optionally, the recombinant microorganisms include at least one nucleic acid sequence encoding a xylulose kinase and/or at least one nucleic acid sequence encoding a xylose transporter. The provided recombinant microorganisms are capable of growing on xylose as a carbon source.
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公开(公告)号：US20170015988A1
公开(公告)日：2017-01-19
申请号：US15208849
申请日：2016-07-13
发明人： Alexandra Merkx-Jacques , David Woodhall , Mark Scaife , Roberto E. Armenta , Denise Muise , Holly Rasmussen , Jeremy Benjamin
CPC分类号： C12N9/92 , C07K14/40 , C07K2319/21 , C12N9/1205 , C12Y207/01017 , C12Y503/01005
摘要： Provided herein are recombinant microorganisms having two or more copies of a nucleic acid sequence encoding xylose isomerase, wherein the nucleic acid encoding the xylose isomerase is an exogenous nucleic acid. Optionally, the recombinant microorganisms include at least one nucleic acid sequence encoding a xylulose kinase and/or at least one nucleic acid sequence encoding a xylose transporter. The provided recombinant microorganisms are capable of growing on xylose as a carbon source.
摘要翻译： 本文提供了具有两个或多个拷贝的编码木糖异构酶的核酸序列的重组微生物，其中编码木糖异构酶的核酸是外源核酸。 任选地，重组微生物包括编码木酮糖激酶和/或编码木糖转运蛋白的至少一个核酸序列的至少一个核酸序列。 所提供的重组微生物能够在作为碳源的木糖上生长。
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