公开(公告)号：US20200325465A1

公开(公告)日：2020-10-15

申请号：US16864098

申请日：2020-04-30

申请人： MARA Renewables Corporation

发明人： Alexandra Merkx-Jacques ,  David Woodhall ,  Mark Scaife ,  Roberto E. Armenta ,  Denise Muise ,  Holly Rasmussen ,  Jeremy Benjamin

IPC分类号： C12N9/92 ,  C12N9/12 ,  C07K14/40 ,  C12P7/64 ,  C07K14/38 ,  C12N1/12 ,  C12N1/10

摘要： Provided herein are recombinant microorganisms having two or more copies of a nucleic acid sequence encoding xylose isomerase, wherein the nucleic acid encoding the xylose isomerase is an exogenous nucleic acid. Optionally, the recombinant microorganisms include at least one nucleic acid sequence encoding a xylulose kinase and/or at least one nucleic acid sequence encoding a xylose transporter. The provided recombinant microorganisms are capable of growing on xylose as a carbon source.

公开(公告)号：US20240182934A1

公开(公告)日：2024-06-06

申请号：US18525184

申请日：2023-11-30

申请人： MARA Renewables Corporation

发明人： Holly Rasmussen ,  David Woodhall ,  Jeremy Benjamin ,  Denise Muise ,  Roberto E. Armenta

IPC分类号： C12P7/6431 ,  C12N1/20 ,  C12N9/02 ,  C12N9/10 ,  C12N15/52 ,  C12P7/6432

CPC分类号： C12P7/6431 ,  C12N1/205 ,  C12N9/0071 ,  C12N9/1029 ,  C12N15/52 ,  C12P7/6432 ,  C12Y114/19001 ,  C12Y114/19006

摘要： Provided herein are engineered microorganisms comprising one or more genes involved in fatty acid metabolism. For example, provided is an engineered microorganism comprising a first nucleic acid sequence encoding an elongase and a second nucleic acid sequence encoding a desaturase, wherein the first and second nucleic acid sequences are operably linked to promoter. Also provided are methods of making and using the engineered microorganisms. Also provided are microbial oils comprising fatty acids, wherein the fatty acids comprise C20:3(n-6) (di-homo-γ-linoleic acid) and C20:5(n-3) eicosapentaenoic acid (EPA). In addition, provided herein are methods of promoting conversion of saturated fatty acids to unsaturated fatty acids by transforming microorganisms with nucleic acid encoding polypeptides involved in the fatty acid synthesis pathway to generated increased conversion of saturated fatty acids to unsaturated fatty acids.

公开(公告)号：US10662418B2

公开(公告)日：2020-05-26

申请号：US15918786

申请日：2018-03-12

申请人： MARA Renewables Corporation

发明人： Alexandra Merkx-Jacques ,  David Woodhall ,  Mark Scaife ,  Roberto E. Armenta ,  Denise Muise ,  Holly Rasmussen ,  Jeremy Benjamin

IPC分类号： C12N9/92 ,  C12N9/12 ,  C07K14/40 ,  C12P7/64 ,  C07K14/38 ,  C12N1/12 ,  C12N1/10

摘要： Provided herein are recombinant microorganisms having two or more copies of a nucleic acid sequence encoding xylose isomerase, wherein the nucleic acid encoding the xylose isomerase is an exogenous nucleic acid. Optionally, the recombinant microorganisms include at least one nucleic acid sequence encoding a xylulose kinase and/or at least one nucleic acid sequence encoding a xylose transporter. The provided recombinant microorganisms are capable of growing on xylose as a carbon source.

公开(公告)号：US20180291363A1

公开(公告)日：2018-10-11

申请号：US15918786

申请日：2018-03-12

申请人： MARA Renewables Corporation

发明人： Alexandra Merkx-Jacques ,  David Woodhall ,  Mark Scaife ,  Roberto E. Armenta ,  Denise Muise ,  Holly Rasmussen ,  Jeremy Benjamin

IPC分类号： C12N9/92 ,  C07K14/40 ,  C12N9/12

摘要： Provided herein are recombinant microorganisms having two or more copies of a nucleic acid sequence encoding xylose isomerase, wherein the nucleic acid encoding the xylose isomerase is an exogenous nucleic acid. Optionally, the recombinant microorganisms include at least one nucleic acid sequence encoding a xylulose kinase and/or at least one nucleic acid sequence encoding a xylose transporter. The provided recombinant microorganisms are capable of growing on xylose as a carbon source.

公开(公告)号：US09951326B2

公开(公告)日：2018-04-24

申请号：US15208849

申请日：2016-07-13

申请人： MARA Renewables Corporation

发明人： Alexandra Merkx-Jacques ,  David Woodhall ,  Mark Scaife ,  Roberto E. Armenta ,  Denise Muise ,  Holly Rasmussen ,  Jeremy Benjamin

IPC分类号： C12N1/11 ,  C12N9/92 ,  C12N9/12 ,  C07K14/40

CPC分类号： C12N9/92 ,  C07K14/40 ,  C07K2319/21 ,  C12N9/1205 ,  C12Y207/01017 ,  C12Y503/01005

摘要： Provided herein are recombinant microorganisms having two or more copies of a nucleic acid sequence encoding xylose isomerase, wherein the nucleic acid encoding the xylose isomerase is an exogenous nucleic acid. Optionally, the recombinant microorganisms include at least one nucleic acid sequence encoding a xylulose kinase and/or at least one nucleic acid sequence encoding a xylose transporter. The provided recombinant microorganisms are capable of growing on xylose as a carbon source.

公开(公告)号：US20170015988A1

公开(公告)日：2017-01-19

申请号：US15208849

申请日：2016-07-13

申请人： MARA Renewables Corporation

发明人： Alexandra Merkx-Jacques ,  David Woodhall ,  Mark Scaife ,  Roberto E. Armenta ,  Denise Muise ,  Holly Rasmussen ,  Jeremy Benjamin

IPC分类号： C12N9/92 ,  C07K14/40 ,  C12N9/12

CPC分类号： C12N9/92 ,  C07K14/40 ,  C07K2319/21 ,  C12N9/1205 ,  C12Y207/01017 ,  C12Y503/01005

摘要： Provided herein are recombinant microorganisms having two or more copies of a nucleic acid sequence encoding xylose isomerase, wherein the nucleic acid encoding the xylose isomerase is an exogenous nucleic acid. Optionally, the recombinant microorganisms include at least one nucleic acid sequence encoding a xylulose kinase and/or at least one nucleic acid sequence encoding a xylose transporter. The provided recombinant microorganisms are capable of growing on xylose as a carbon source.

摘要翻译： 本文提供了具有两个或多个拷贝的编码木糖异构酶的核酸序列的重组微生物，其中编码木糖异构酶的核酸是外源核酸。 任选地，重组微生物包括编码木酮糖激酶和/或编码木糖转运蛋白的至少一个核酸序列的至少一个核酸序列。 所提供的重组微生物能够在作为碳源的木糖上生长。