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    Control of foam during growth of a microorganism
    1.
    发明授权
    Control of foam during growth of a microorganism 失效
    泡沫在微生物生长过程中的控制

    公开(公告)号：US3841972A

    公开(公告)日：1974-10-15

    申请号：US29068972

    申请日：1972-09-20

    Applicant: MOBIL OIL CORP

    Inventor： CHEN N ,  LEAVITT R ,  COTY V

    IPC: C12N1/26 ,  C12N1/38 ,  C12B1/18

    CPC classification number: C12N1/26 ,  C12N1/38 ,  Y10S435/812 ,  Y10S435/911 ,  Y10S435/923 ,  Y10S435/938

    Abstract: 1. IN A PROCESS FOR THE GROWTH OF CELLS OF AN AEROBIC MICROORGANISM IN AN AGITATED CULTURE MEDIUM CONTAINED IN A FERMENTED AND IN CONTACT WITH AN OXYGEN-CONTAINING GAS, SAID CULTURE MEDIUM CONTAINING AN INOCULUM OF SAID MICROORGANISM, AN AQUEOUS MINERAL SALT NUTRIENT, AND A LIQUID HYDROCARBON SUBSTRATE, THE IMPROVEMENT FOR CONTROLLING THE FORMATION OF FOAM IN SAID CULTURE MEDIUM DURING GROWTH OF SAID CELLS OF SAID MICROORGANISM COMPRISING CIRCULATING SAID CULTURE MEDIUM OVER A SOLID, UNIT BODY HAVING A MINIMUM DIMENSION OF 0.001 INCH OF A POLYMER HAVING A MOLECULAR WEIGHT ABOVE 25,000 SELECTED FROM THE GROUP CONSISTING OF POLYETHYLENE, POLYPROPYLENE, POLYBUTYLENE, AND POLYSTYRENE, SAID SOLID,UNIT BODY BEING IN THE FORM OF A FILAMENT, A DISTINCT SHAPE, OR AN INDISCRIMINATE SHAPE.

    Tants obtained therefrom method of isolating amino acid producing mutant microorganisms and mu
    2.
    发明授权
    Tants obtained therefrom method of isolating amino acid producing mutant microorganisms and mu 失效
    分离氨基酸生产突变型微生物和微生物的方法

    公开(公告)号：US3756916A

    公开(公告)日：1973-09-04

    申请号：US3756916D

    申请日：1971-03-29

    Applicant: MOBIL OIL CORP

    Inventor： LEAVITT R

    IPC: C12P13/04 ,  C12P13/08 ,  C12B1/00

    CPC classification number: C12P13/08 ,  C12P13/04

    Abstract: A METHOD OF ISOLATING A MUTANT STRAIN OF AN AMINO ACID-PRODUCING MICROORGANISM, WHICH MUTANT PRODUCES SAID AMINO ACID AT A GREATER RATE THAN NORMALLY PRODUCED BY ITS MICROORGANISM PARENT, BY THE STEP OF CULTURING THE MICROORGANISM PARENT IN THE PRESENCE OF (1) AN ANTIMETABOLITE AND (2) A CO-INHIBITOR AGENT, THEREBY INHIBITING THE GROWTH OF THE NORMAL MICROORGANISM, ISOLATING AND SUBCULTURING THE MUTANT AND OBTAINING THE DESIRED AMINO ACID.

    Method for producing hydrocarbon-utilizing yeasts
    3.
    发明授权
    Method for producing hydrocarbon-utilizing yeasts 失效
    生产油料利用方法

    公开(公告)号：US3767527A

    公开(公告)日：1973-10-23

    申请号：US3767527D

    申请日：1971-10-07

    Applicant: MOBIL OIL CORP

    Inventor： COTY V ,  LEAVITT R

    IPC: C12N1/26 ,  C12B1/00

    CPC classification number: C12N1/26 ,  Y10S435/911 ,  Y10S435/923 ,  Y10S435/938

    Abstract: This specification discloses a method for producing aerobic, hydrocarbon-utilizing yeasts. The yeasts are grown in an oxygencontaining incubation zone in an agitated culture mixture in the form of an oil phase-continuous emulsion of oil and water. The oil phase of the emulsion comprises a hydrocarbon and the water phase comprises an aqueous mineral nutrient solution. The pH of the emulsion is maintained in the range of 2-4 during growth of the yeasts and the yeasts are grown to a desired extent but not to a point where the emulsion inverts into a water phasecontinuous emulsion. The pH of the emulsion is then raised at least one-half pH unit into the range of above 4 but not above 5, thereby separating the emulsion into an oil phase and a water phase, the water phase containing the yeast cells. The yeast cells are then recovered from the water phase.

    Abstract translation: 本说明书公开了一种生产好氧，含烃的酵母菌的方法。 将酵母在含氧培养区中在油相和水相的油相连续乳液形式的搅拌培养混合物中生长。 乳液的油相包含烃，水相包含含水矿物营养液。 在酵母生长期间，乳液的pH值保持在2-4范围内，并且酵母菌生长至所需的程度，但不到乳液反转为水相连续乳液的程度。 然后将乳液的pH升高至少一个pH单位至4以上但不高于5的范围，从而将乳液分离成油相和水相，含有酵母细胞的水相。 然后从水相中回收酵母细胞。

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