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公开(公告)号：US20050214914A1
公开(公告)日：2005-09-29
申请号：US10499819
申请日：2002-12-27
申请人： Manabu Sato , Masami Shimizu , Jun Kohori , Minoru Kase , Takaaki Watanabe , Kazuhiro Onozuka
发明人： Manabu Sato , Masami Shimizu , Jun Kohori , Minoru Kase , Takaaki Watanabe , Kazuhiro Onozuka
CPC分类号： C12P7/6454 , C12P7/62
摘要： Provided is a preparation process of diglycerides which comprises reacting a fatty acid or lower alkyl ester thereof with glycerin in the presence of an immobilized partial glyceride lipase while removing water, which is generated upon reaction, out of the system. The reaction is preferably terminated when the acid value (AV) of the reaction mixture satisfies the following range: 50R-60>AV>70R-150 (with the proviso that AV>0). The present process makes it possible to efficiently prepare a high purity of diglycerides without impairing lipase activity even under reduced pressure. The present process is effective without being influenced by variations in reaction conditions such as fatty acid/glycerin ratio, reaction temperature or enzyme concentration. Particularly, even when the fatty acid/glycerin ratio is high, a high purity of diglycerides can be produced at a high reaction yield.
摘要翻译： 提供了一种甘油二酯的制备方法，其包括在固定化的部分甘油酯脂肪酶的存在下使脂肪酸或其低级烷基酯与甘油反应，同时将反应后产生的水除去系统。 当反应混合物的酸值（AV）满足以下范围：50R-60> AV> 70R-150（条件是AV> 0）时，反应优选终止。 本方法使得即使在减压下也能有效地制备高纯度甘油二酯而不损害脂肪酶活性。 本方法不受脂肪酸/甘油比，反应温度或酶浓度等反应条件的变化的影响而有效。 特别是，即使脂肪酸/甘油的比例高，也能够以高的反应收率生产高纯度的甘油二酯。
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公开(公告)号：US07141399B2
公开(公告)日：2006-11-28
申请号：US10499819
申请日：2002-12-27
申请人： Manabu Sato , Masami Shimizu , Jun Kohori , Minoru Kase , Takaaki Watanabe , Kazuhiro Onozuka
发明人： Manabu Sato , Masami Shimizu , Jun Kohori , Minoru Kase , Takaaki Watanabe , Kazuhiro Onozuka
IPC分类号： C12P7/64
CPC分类号： C12P7/6454 , C12P7/62
摘要： Provided is a preparation process of diglycerides which comprises reacting a fatty acid or lower alkyl ester thereof with glycerin in the presence of an immobilized partial glyceride lipase while removing water, which is generated upon reaction, out of the system. The reaction is preferably terminated when the acid value (AV) of the reaction mixture satisfies the following range: 50R-60>AV>70R-150 (with the proviso that AV>0). The present process makes it possible to efficiently prepare a high purity of diglycerides without impairing lipase activity even under reduced pressure. The present process is effective without being influenced by variations in reaction conditions such as fatty acid/glycerin ratio, reaction temperature or enzyme concentration. Particularly, even when the fatty acid/glycerin ratio is high, a high purity of diglycerides can be produced at a high reaction yield.
摘要翻译： 提供了一种甘油二酯的制备方法，其包括在固定化的部分甘油酯脂肪酶的存在下使脂肪酸或其低级烷基酯与甘油反应，同时将反应后产生的水除去系统。 当反应混合物的酸值（AV）满足以下范围：50R-60> AV> 70R-150（条件是AV> 0）时，反应优选终止。 本方法使得即使在减压下也能有效地制备高纯度甘油二酯而不损害脂肪酶活性。 本方法不受脂肪酸/甘油比，反应温度或酶浓度等反应条件的变化的影响而有效。 特别是，即使脂肪酸/甘油的比例高，也能够以高的反应收率生产高纯度的甘油二酯。
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公开(公告)号：US07906305B2
公开(公告)日：2011-03-15
申请号：US11668182
申请日：2007-01-29
申请人： Manabu Sato , Masami Shimizu , Minoru Kase , Takaaki Watanabe , Jun Kohori
发明人： Manabu Sato , Masami Shimizu , Minoru Kase , Takaaki Watanabe , Jun Kohori
IPC分类号： C12N11/00
摘要： Provided is a process for preparing an immobilized enzyme, which comprises the steps of immobilizing an enzyme used for decomposing oil & fat on a carrier, by adsorption, without drying, bringing the immobilized enzyme into contact with a fatty acid triglyceride or fatty acid partial glyceride, or mixtures thereof, and adjusting the moisture content of the enzyme to 5% to 50% by weight based on the weight of the carrier, wherein the enzyme is used for esterification.
摘要翻译： 提供一种制备固定化酶的方法，其包括以下步骤：将用于分解油脂的酶固定在载体上，通过吸附而不干燥，使固定化酶与脂肪酸甘油三酯或脂肪酸偏甘油酯接触 ，或其混合物，并且基于载体的重量将酶的水分含量调节至5重量％至50重量％，其中该酶用于酯化。
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公开(公告)号：US20070122893A1
公开(公告)日：2007-05-31
申请号：US11668182
申请日：2007-01-29
申请人： Manabu Sato , Masami Shimizu , Minoru Kase , Takaaki Watanabe , Jun Kohori
发明人： Manabu Sato , Masami Shimizu , Minoru Kase , Takaaki Watanabe , Jun Kohori
IPC分类号： C12N11/04
摘要： Provided is a process for preparing an immobilized enzyme, which comprises the steps of immobilizing an enzyme used for decomposing oil & fat on a carrier, by adsorption, without drying, bringing the immobilized enzyme into contact with a fatty acid triglyceride or fatty acid partial glyceride, or mixtures thereof, and adjusting the moisture content of the enzyme to 5% to 50% by weight based on the weight of the carrier, wherein the enzyme is used for esterification.
摘要翻译： 提供一种制备固定化酶的方法，其包括以下步骤：将用于分解油脂的酶固定在载体上，通过吸附而不干燥，使固定化酶与脂肪酸甘油三酯或脂肪酸偏甘油酯接触 ，或其混合物，并且基于载体的重量将酶的水分含量调节至5重量％至50重量％，其中该酶用于酯化。
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公开(公告)号：US07238504B2
公开(公告)日：2007-07-03
申请号：US10609401
申请日：2003-07-01
申请人： Manabu Sato , Masami Shimizu , Minoru Kase , Takaaki Watanabe , Jun Kohori
发明人： Manabu Sato , Masami Shimizu , Minoru Kase , Takaaki Watanabe , Jun Kohori
IPC分类号： C12N11/00
摘要： Provided is a process for preparing an immobilized enzyme, which comprises the steps of immobilizing an enzyme used for decomposing oil & fat on a carrier, by adsorption, without drying, bringing the immobilized enzyme into contact with a fatty acid triglyceride or fatty acid partial glyceride, or mixtures thereof, and adjusting the moisture content of the enzyme to 5% to 50% by weight based on the weight of the carrier, wherein the enzyme is used for esterification.
摘要翻译： 提供一种制备固定化酶的方法，其包括以下步骤：将用于分解油脂的酶固定在载体上，通过吸附而不干燥，使固定化酶与脂肪酸甘油三酯或脂肪酸偏甘油酯接触 ，或其混合物，并且基于载体的重量将酶的水分含量调节至5重量％至50重量％，其中该酶用于酯化。
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公开(公告)号：US20050019883A1
公开(公告)日：2005-01-27
申请号：US10609401
申请日：2003-07-01
申请人： Manabu Sato , Masami Shimizu , Minoru Kase , Takaaki Watanabe , Jun Kohori
发明人： Manabu Sato , Masami Shimizu , Minoru Kase , Takaaki Watanabe , Jun Kohori
摘要： Provided is a process for preparing an immobilized enzyme, which comprises the steps of immobilizing an enzyme used for decomposing oil & fat on a carrier, by adsorption, without drying, bringing the immobilized enzyme into contact with a fatty acid triglyceride or fatty acid partial glyceride, or mixtures thereof, and adjusting the moisture content of the enzyme to 5% to 50% by weight based on the weight of the carrier, wherein the enzyme is used for esterification.
摘要翻译： 提供一种制备固定化酶的方法，其包括以下步骤：将用于分解油脂的酶固定在载体上，通过吸附而不干燥，使固定化酶与脂肪酸甘油三酯或脂肪酸偏甘油酯接触 ，或其混合物，并且基于载体的重量将酶的水分含量调节至5重量％至50重量％，其中该酶用于酯化。
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7.发明授权公开(公告)号：US07807428B2
公开(公告)日：2010-10-05
申请号：US11270497
申请日：2005-11-10
摘要： A method for producing an immobilized enzyme which includes the steps of immobilizing an enzyme used for hydrolyzing fats and oils on a immobilization carrier by adsorption, bringing the immobilized enzyme into contact with an organic solvent in which fat-soluble fatty acids or the derivatives thereof have been dissolved, and adjusting the moisture content of the immobilized enzyme from 1 to 20% by weight based on the weight of the carrier.
摘要翻译： 一种固定化酶的制造方法，其特征在于，包括以下步骤：通过吸附将固定化载体上的水解油脂的酶固定化，使固定化酶与脂溶性脂肪酸或其衍生物具有的有机溶剂接触 已经溶解，并且基于载体的重量将固定化酶的水分含量调整为1至20重量％。
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公开(公告)号：US20060121584A1
公开(公告)日：2006-06-08
申请号：US11270497
申请日：2005-11-10
摘要： A method for producing an immobilized enzyme which includes the steps of immobilizing an enzyme used for hydrolyzing fats and oils on a immobilization carrier by adsorption, bringing the immobilized enzyme into contact with an organic solvent in which fat-soluble fatty acids or the derivatives thereof have been dissolved, and adjusting the moisture content of the immobilized enzyme from 1 to 20% by weight based on the weight of the carrier.
摘要翻译： 一种固定化酶的制造方法，其特征在于，包括以下步骤：通过吸附将固定化载体上的水解油脂的酶固定化，使固定化酶与脂溶性脂肪酸或其衍生物具有的有机溶剂接触 已经溶解，并且基于载体的重量将固定化酶的水分含量调整为1至20重量％。
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9.发明授权公开(公告)号：US08415124B2
公开(公告)日：2013-04-09
申请号：US12839711
申请日：2010-07-20
摘要： A method for producing an immobilized enzyme which includes the steps of immobilizing an enzyme used for hydrolyzing fats and oils on a immobilization carrier by adsorption, bringing the immobilized enzyme into contact with an organic solvent in which fat-soluble fatty acids or the derivatives thereof have been dissolved, and adjusting the moisture content of the immobilized enzyme from 1 to 20% by weight based on the weight of the carrier.
摘要翻译： 一种固定化酶的制造方法，其特征在于，包括以下步骤：通过吸附将固定化载体上的水解油脂的酶固定化，使固定化酶与脂溶性脂肪酸或其衍生物具有的有机溶剂接触 已经溶解，并且基于载体的重量将固定化酶的水分含量调整为1至20重量％。
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公开(公告)号：US20100028964A1
公开(公告)日：2010-02-04
申请号：US12529716
申请日：2008-03-05
IPC分类号： C12P7/02
CPC分类号： C11B9/0053 , C12P7/22
摘要： The present invention relates to a method for production of a diol form represented by the formula (2), which includes filtering a culture fluid obtained by microbial conversion using a compound represented by the following formula (1a) and/or formula (1b) as a substrate, using a filter having an aperture size of 10 to 100 μm; washing the residue on the filter with water or a solvent having an SP value outside the range of 8.3 to 20 (cal/cm3)1/2; subsequently dissolving the obtained cake in a solvent having an SP value of 8.3 to 20 (cal/cm3)1/2; and then filtering or centrifuging the solution.According to the present invention, 1-(2-hydroxyethyl)-2,5,5,8a-tetramethyldecahydronaphthalen-2-ol, which is useful as an intermediate for the production of 3a,6,6,9a-tetramethyldodecahydronaphtho[2,1-b]furan, can be efficiently produced at high purity.
摘要翻译： 本发明涉及一种由式（2）表示的二醇形式的制备方法，其包括使用下式（1a）和/或式（1b）表示的化合物将通过微生物转化获得的培养液过滤为 使用孔径为10〜100μm的滤光片的基板; 用水或SP值在8.3〜20（cal / cm3）1/2以下的溶剂洗涤过滤器上的残留物; 随后将得到的滤饼溶解在SP值为8.3〜20（cal / cm 3）1/2的溶剂中; 然后过滤或离心溶液。 根据本发明，可用作制备3a，6,6,9a-四甲基十二氢萘并[2,5-二氮杂萘基]的中间体的1-（2-羟乙基）-2,5,5,8a-四甲基十氢萘-2-醇， 1-b]呋喃可以高纯度地高效生产。
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