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    Method for fabricating microbeads and microbeads
    2.
    发明授权
    Method for fabricating microbeads and microbeads 有权
    微珠和微珠的制备方法

    公开(公告)号：US08546068B2

    公开(公告)日：2013-10-01

    申请号：US13120967

    申请日：2009-09-04

    申请人： Mari Ichimura ,  Kenzo Machida ,  Noriyuki Kishii ,  Masanobu Tanaka

    发明人： Mari Ichimura ,  Kenzo Machida ,  Noriyuki Kishii ,  Masanobu Tanaka

    IPC分类号： G03F7/26

    CPC分类号： G01N33/54313 ,  B01J2219/00432 ,  B01J2219/005 ,  B01J2219/00502 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00637 ,  B01J2219/00677 ,  B01J2219/00722

    摘要： In one example embodiment, a method fabricates microbeads, which can supply a bead set containing a various types of microbeads and having distinct populations of the respective types of microbeads. In one example embodiment, the method includes forming a hydrophilic layer made of a hydrophilic organic material on a substrate. In one example embodiment, the method includes laminating on the hydrophilic layer a thin film capable of being peeled off in the form of microbeads. In one example embodiment, the method includes forming the thin film in a given configuration by photolithography. In one example embodiment, the method includes solid-phasing a given substance on the post-formed thin films. In one example embodiment, the method includes peeling off the post-formed thin films, which have been solid-phased with the substance, from the substrate along with at least a part of the hydrophilic layer to obtain microbeads.

    摘要翻译： 在一个示例性实施例中，一种制造微珠的方法，其可以提供包含各种类型的微珠并且具有各种类型的微珠的不同种群的珠粒组。 在一个示例性实施例中，该方法包括在基底上形成由亲水性有机材料制成的亲水层。 在一个示例性实施例中，该方法包括在亲水层上层叠能够以微珠形式剥离的薄膜。 在一个示例性实施例中，该方法包括通过光刻法以给定的配置形成薄膜。 在一个示例性实施例中，该方法包括将定影物质固定在后成形薄膜上。 在一个示例性实施例中，该方法包括将已经与物质固相化的后成形薄膜与至少一部分亲水层一起从衬底剥离以获得微珠。

    MICROBEAD AUTOMATIC RECOGNITION METHOD AND MICROBEAD
    4.
    发明申请
    MICROBEAD AUTOMATIC RECOGNITION METHOD AND MICROBEAD 有权
    MICROBEAD自动识别方法和MICROBEAD

    公开(公告)号：US20120195507A1

    公开(公告)日：2012-08-02

    申请号：US13440366

    申请日：2012-04-05

    申请人： Noriyuki Kishii ,  Mari Ichimura ,  Kenzo Machida

    发明人： Noriyuki Kishii ,  Mari Ichimura ,  Kenzo Machida

    IPC分类号： G06K9/46

    CPC分类号： G06K19/06037 ,  G06T7/74 ,  G06T2207/30072 ,  G06T2207/30208 ,  Y10T428/2991

    摘要： A microbead automatic recognition method includes the steps of: acquiring an image of a circular surface of a cylindrical microbead having a recognition pattern created on the circular surface and a plurality of reference points also created on the circular surface; and acquiring information on the rear/front and/or orientation of the cylindrical microbead from the acquired image on the basis of the positions of the reference points.

    摘要翻译： 微珠自动识别方法包括以下步骤：获取具有在圆形表面上产生的识别图案的圆柱形微珠的圆形表面的图像和在圆形表面上也产生的多个参考点; 并且根据参考点的位置从获取的图像获取关于圆柱形微珠的后/前和/或取向的信息。

    Microbead analysis method and microbead analyzer
    5.
    发明授权
    Microbead analysis method and microbead analyzer 失效
    微珠分析法和微珠分析仪

    公开(公告)号：US08753898B2

    公开(公告)日：2014-06-17

    申请号：US12877237

    申请日：2010-09-08

    申请人： Kenzo Machida ,  Noriyuki Kishii ,  Mari Ichimura ,  Kazumine Ito ,  Takuya Kishimoto ,  Naohisa Sakamoto

    发明人： Kenzo Machida ,  Noriyuki Kishii ,  Mari Ichimura ,  Kazumine Ito ,  Takuya Kishimoto ,  Naohisa Sakamoto

    IPC分类号： G01N33/543

    CPC分类号： G01N33/54366 ,  G01N21/6452

    摘要： Provided is a microbead analysis method for a microbead. The microbead is formed in a columnar shape having a top surface and a bottom surface facing each other, as placed almost in parallel, and a side surface extending therefrom, and carries an identification pattern formed on at least one of the top surface and the bottom surface and a substance immobilized on a surface thereof having affinity to an analyte substance. The method includes detecting fluorescence emitted from the microbead surface due to interaction of the analyte substance with the substance having affinity to the analyte substance from a region including a region of the top surface and the bottom surface where there is no identification pattern formed and the side surface.

    摘要翻译： 微珠的微珠分析方法。 微珠形成为具有彼此面对的顶表面和底表面的几乎平行的柱状和从其延伸的侧表面，并且携带形成在顶表面和底部中的至少一个上的识别图案 表面和固定在其对分析物质具有亲和性的表面上的物质。 该方法包括检测由于分析物质与对分析物质具有亲和性的物质的相互作用而从微珠表面发射的荧光，该区域包括不形成识别图案的顶表面和底表面的区域，侧面 表面。

    Microbead automatic recognition method and microbead
    6.
    发明授权
    Microbead automatic recognition method and microbead 有权
    微珠自动识别方法和微珠

    公开(公告)号：US08548221B2

    公开(公告)日：2013-10-01

    申请号：US13440366

    申请日：2012-04-05

    申请人： Noriyuki Kishii ,  Mari Ichimura ,  Kenzo Machida

    发明人： Noriyuki Kishii ,  Mari Ichimura ,  Kenzo Machida

    IPC分类号： G06K9/00

    CPC分类号： G06K19/06037 ,  G06T7/74 ,  G06T2207/30072 ,  G06T2207/30208 ,  Y10T428/2991

    摘要： A microbead automatic recognition method includes the steps of: acquiring an image of a circular surface of a cylindrical microbead having a recognition pattern created on the circular surface and a plurality of reference points also created on the circular surface; and acquiring information on the rear/front and/or orientation of the cylindrical microbead from the acquired image on the basis of the positions of the reference points.

    摘要翻译： 微珠自动识别方法包括以下步骤：获取具有在圆形表面上产生的识别图案的圆柱形微珠的圆形表面的图像和在圆形表面上也产生的多个参考点; 并且根据参考点的位置从获取的图像获取关于圆柱形微珠的后/前和/或取向的信息。

    Microbead automatic recognition method and microbead
    7.
    发明授权
    Microbead automatic recognition method and microbead 有权
    微珠自动识别方法和微珠

    公开(公告)号：US08200000B2

    公开(公告)日：2012-06-12

    申请号：US12432121

    申请日：2009-04-29

    申请人： Noriyuki Kishii ,  Mari Ichimura ,  Kenzo Machida

    发明人： Noriyuki Kishii ,  Mari Ichimura ,  Kenzo Machida

    IPC分类号： G06K9/00

    CPC分类号： G06K19/06037 ,  G06T7/74 ,  G06T2207/30072 ,  G06T2207/30208 ,  Y10T428/2991

    摘要： A microbead automatic recognition method includes the steps of: acquiring an image of a circular surface of a cylindrical microbead having a recognition pattern created on the circular surface and a plurality of reference points also created on the circular surface; and acquiring information on the rear/front and/or orientation of the cylindrical microbead from the acquired image on the basis of the positions of the reference points.

    摘要翻译： 微珠自动识别方法包括以下步骤：获取具有在圆形表面上产生的识别图案的圆柱形微珠的圆形表面的图像和在圆形表面上也产生的多个参考点; 并且根据参考点的位置从获取的图像获取关于圆柱形微珠的后/前和/或取向的信息。

    MICROBEAD ANALYSIS METHOD AND MICROBEAD ANALYZER
    8.
    发明申请
    MICROBEAD ANALYSIS METHOD AND MICROBEAD ANALYZER 失效
    MICROBEAD分析方法和MICROBEAD分析仪

    公开(公告)号：US20110136263A1

    公开(公告)日：2011-06-09

    申请号：US12877237

    申请日：2010-09-08

    申请人： Kenzo Machida ,  Noriyuki Kishii ,  Mari Ichimura ,  Kazumine Ito ,  Takuya Kishimoto ,  Naohisa Sakamoto

    发明人： Kenzo Machida ,  Noriyuki Kishii ,  Mari Ichimura ,  Kazumine Ito ,  Takuya Kishimoto ,  Naohisa Sakamoto

    IPC分类号： G01N33/543 ,  G01N21/64

    CPC分类号： G01N33/54366 ,  G01N21/6452

    摘要： Provided is a microbead analysis method for a microbead. The microbead is formed in a columnar shape having a top surface and a bottom surface facing each other, as placed almost in parallel, and a side surface extending therefrom, and carries an identification pattern formed on at least one of the top surface and the bottom surface and a substance immobilized on a surface thereof having affinity to an analyte substance. The method includes detecting fluorescence emitted from the microbead surface due to interaction of the analyte substance with the substance having affinity to the analyte substance from a region including a region of the top surface and the bottom surface where there is no identification pattern formed and the side surface.

    摘要翻译： 微珠的微珠分析方法。 微珠形成为具有彼此面对的顶表面和底表面的几乎平行的柱状和从其延伸的侧表面，并且携带形成在顶表面和底部中的至少一个上的识别图案 表面和固定在其对分析物质具有亲和性的表面上的物质。 该方法包括检测由于分析物质与对分析物质具有亲和性的物质的相互作用而从微珠表面发射的荧光，该区域包括不形成识别图案的顶表面和底表面的区域，侧面 表面。

    METHOD FOR FABRICATING MICROBEADS AND MICROBEADS
    9.
    发明申请
    METHOD FOR FABRICATING MICROBEADS AND MICROBEADS 有权
    制备微生物和微生物的方法

    公开(公告)号：US20110183272A1

    公开(公告)日：2011-07-28

    申请号：US13120967

    申请日：2009-09-04

    申请人： Mari Ichimura ,  Kenzo Machida ,  Noriyuki Kishii ,  Masannobu Tanaka

    发明人： Mari Ichimura ,  Kenzo Machida ,  Noriyuki Kishii ,  Masannobu Tanaka

    IPC分类号： G03F7/20

    CPC分类号： G01N33/54313 ,  B01J2219/00432 ,  B01J2219/005 ,  B01J2219/00502 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00637 ,  B01J2219/00677 ,  B01J2219/00722

    摘要： In one example embodiment, a method fabricates microbeads, which can supply a bead set containing a various types of microbeads and having distinct populations of the respective types of microbeads. In one example embodiment, the method includes forming a hydrophilic layer made of a hydrophilic organic material on a substrate. In one example embodiment, the method includes laminating on the hydrophilic layer a thin film capable of being peeled off in the form of microbeads. In one example embodiment, the method includes forming the thin film in a given configuration by photolithography. In one example embodiment, the method includes solid-phasing a given substance on the post-formed thin films. In one example embodiment, the method includes peeling off the post-formed thin films, which have been solid-phased with the substance, from the substrate along with at least a part of the hydrophilic layer to obtain microbeads.

    摘要翻译： 在一个示例性实施例中，一种制造微珠的方法，其可以提供包含各种类型的微珠并且具有各种类型的微珠的不同种群的珠粒组。 在一个示例性实施例中，该方法包括在基底上形成由亲水性有机材料制成的亲水层。 在一个示例性实施例中，该方法包括在亲水层上层叠能够以微珠形式剥离的薄膜。 在一个示例性实施例中，该方法包括通过光刻法以给定的配置形成薄膜。 在一个示例性实施例中，该方法包括将定影物质固定在后成形薄膜上。 在一个示例性实施例中，该方法包括将已经与物质固相化的后成形薄膜与至少一部分亲水层一起从衬底剥离以获得微珠。

    MICROBEAD AUTOMATIC RECOGNITION METHOD AND MICROBEAD
    10.
    发明申请
    MICROBEAD AUTOMATIC RECOGNITION METHOD AND MICROBEAD 有权
    MICROBEAD自动识别方法和MICROBEAD

    公开(公告)号：US20090279787A1

    公开(公告)日：2009-11-12

    申请号：US12432121

    申请日：2009-04-29

    申请人： Noriyuki Kishii ,  Mari Ichimura ,  Kenzo Machida

    发明人： Noriyuki Kishii ,  Mari Ichimura ,  Kenzo Machida

    IPC分类号： G06K9/46 ,  B32B1/00

    CPC分类号： G06K19/06037 ,  G06T7/74 ,  G06T2207/30072 ,  G06T2207/30208 ,  Y10T428/2991

    摘要： A microbead automatic recognition method includes the steps of: acquiring an image of a circular surface of a cylindrical microbead having a recognition pattern created on the circular surface and a plurality of reference points also created on the circular surface; and acquiring information on the rear/front and/or orientation of the cylindrical microbead from the acquired image on the basis of the positions of the reference points.

    摘要翻译： 微珠自动识别方法包括以下步骤：获取具有在圆形表面上产生的识别图案的圆柱形微珠的圆形表面的图像和在圆形表面上也产生的多个参考点; 并且根据参考点的位置从获取的图像获取关于圆柱形微珠的后/前和/或取向的信息。