SELECTIVE ABLATION OF DIPLOID EMBRYOS
    4.
    发明申请
    SELECTIVE ABLATION OF DIPLOID EMBRYOS 审中-公开
    胆固醇的选择性消除

    公开(公告)号:US20120311736A1

    公开(公告)日:2012-12-06

    申请号:US13586893

    申请日:2012-08-16

    IPC分类号: A01H1/04 A01H1/02 A01H5/00

    摘要: Methods of selecting haploid embryos are disclosed. Methods of producing haploid embryos and non-viable diploid embryos on a plant are provided. Methods for selecting haploid embryos produced from haploid inducer maize lines are provided. Methods for producing improved maize haploid inducer lines are disclosed. Maize haploid inducer lines comprising transgenes causing ablated or abnormal diploid embryos are disclosed.

    摘要翻译: 公开了选择单倍体胚胎的方法。 提供了在植物上产生单倍体胚胎和不可行的二倍体胚胎的方法。 提供了用于选择由单倍体诱导物玉米品系生产的单倍体胚胎的方法。 公开了生产改良的玉米单倍体诱导物系的方法。 公开了包含导致烧蚀或异常二倍体胚的转基因的玉米单倍体诱导物系。

    Selective ablation of diploid embryos
    5.
    发明授权
    Selective ablation of diploid embryos 有权
    二倍体胚胎的选择性消融

    公开(公告)号:US09121032B2

    公开(公告)日:2015-09-01

    申请号:US13586893

    申请日:2012-08-16

    IPC分类号: A01H1/08 C12N15/82

    摘要: Methods of selecting haploid embryos are disclosed. Methods of producing haploid embryos and non-viable diploid embryos on a plant are provided. Methods for selecting haploid embryos produced from haploid inducer maize lines are provided. Methods for producing improved maize haploid inducer lines are disclosed. Maize haploid inducer lines comprising transgenes causing ablated or abnormal diploid embryos are disclosed.

    摘要翻译: 公开了选择单倍体胚胎的方法。 提供了在植物上产生单倍体胚胎和不可行的二倍体胚胎的方法。 提供了用于选择由单倍体诱导物玉米品系生产的单倍体胚胎的方法。 公开了生产改良的玉米单倍体诱导物系的方法。 公开了包含导致烧蚀或异常二倍体胚的转基因的玉米单倍体诱导物系。

    Selective Ablation of Diploid Embryos
    6.
    发明申请
    Selective Ablation of Diploid Embryos 审中-公开
    二倍体胚胎的选择性消融

    公开(公告)号:US20080189801A1

    公开(公告)日:2008-08-07

    申请号:US12023152

    申请日:2008-01-31

    IPC分类号: A01H1/02 C12N15/29 A01H5/00

    摘要: Methods of selecting haploid embryos are disclosed. Methods of producing haploid embryos and non-viable diploid embryos on a plant are provided. Methods for selecting haploid embryos produced from haploid inducer maize lines are provided. Methods for producing improved maize haploid inducer lines are disclosed. Maize haploid inducer lines comprising transgenes causing ablated or abnormal diploid embryos are disclosed.

    摘要翻译: 公开了选择单倍体胚胎的方法。 提供了在植物上产生单倍体胚胎和不可行的二倍体胚胎的方法。 提供了用于选择由单倍体诱导物玉米品系生产的单倍体胚胎的方法。 公开了生产改良的玉米单倍体诱导物系的方法。 公开了包含导致烧蚀或异常二倍体胚的转基因的玉米单倍体诱导物系。

    METHODS AND COMPOSITIONS FOR TARGETED POLYNUCLEOTIDE MODIFICATION
    8.
    发明申请
    METHODS AND COMPOSITIONS FOR TARGETED POLYNUCLEOTIDE MODIFICATION 有权
    用于靶向多核苷酸修饰的方法和组合物

    公开(公告)号:US20110165679A1

    公开(公告)日:2011-07-07

    申请号:US12982013

    申请日:2010-12-30

    IPC分类号: C12N15/82 C12N5/10

    摘要: A variety of methods and compostions are provided, including methods and compositions for targeted modification of a specific target site in a cell or organism, methods for integrating polynucleotides of interest, methods to assess promoter activity, directly select transformed organisms, minimize or eliminate expression resulting from random integration into the genome of an organism, such as a plant, remove polynucleotides of interest, combine multiple transfer cassettes, invert or excise a polynucleotide, silence a gene, and identify and/or characterize transcriptional regulating regions. The methods involve the introduction of a cell proliferation factor and a double-strand break-inducing enzyme into an organism.

    摘要翻译: 提供了各种方法和组合物,包括用于靶向修饰细胞或生物体中特定靶位点的方法和组合物,用于整合感兴趣的多核苷酸的方法,评估启动子活性的方法,直接选择转化的生物体,最小化或消除产生的表达 从随机整合到生物体如植物的基因组中,去除感兴趣的多核苷酸,组合多个转移盒,反转或切割多核苷酸,使基因沉默,并鉴定和/或表征转录调节区。 该方法涉及将细胞增殖因子和双链断裂诱导酶引入生物体中。