公开(公告)号：US5362625A

公开(公告)日：1994-11-08

申请号：US67342

申请日：1993-05-25

申请人： Mark Krevolin ,  David Kates

发明人： Mark Krevolin ,  David Kates

IPC分类号： C07K14/195 ,  C07K14/00 ,  C07K14/41 ,  C07K19/00 ,  C12N9/38 ,  C12N15/00 ,  C12N15/09 ,  C12Q1/34 ,  G01N33/53 ,  G01N33/58 ,  G01N33/535

CPC分类号： G01N33/581

摘要： The use of omega-acceptor and omega-donor polypeptides (comprising about two-thirds and one-third of the .beta.-galactosidase molecule amino and carboxyl termini, respectively), prepared by recombinant DNA techniques, DNA synthesis, or chemical polypeptide synthesis techniques, which are capable of interacting to form an active enzyme complex having catalytic activity characteristic of .beta.-galactosidase, is described along with improved methods and novel compositions for enzyme complementation assays for qualitative and quantitative determination of a suspected analyte in a sample.

摘要翻译： 通过重组DNA技术，DNA合成或化学多肽合成技术制备的ω-受体和ω-供体多肽（分别包含约三分之二和三分之一的β-半乳糖苷酶分子氨基和羧基末端） 其能够相互作用以形成具有β-半乳糖苷酶特征的催化活性的活性酶复合物，以及改进的方法和用于酶互补测定的新组合物用于定性和定量测定样品中的可疑分析物。