利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

6 条记录 (耗时 0.023 秒)

    Production of insulin-like growth factor-1 in methylotrophic yeast cells
    2.
    发明授权
    Production of insulin-like growth factor-1 in methylotrophic yeast cells 失效
    在甲基营养酵母细胞中生产胰岛素样生长因子-1

    公开(公告)号：US5612198A

    公开(公告)日：1997-03-18

    申请号：US308196

    申请日：1994-09-19

    申请人： Russell A. Brierley ,  Geneva R. Davis ,  Gregory C. Holtz ,  Martin A. Gleeson ,  Bradley D. Howard

    发明人： Russell A. Brierley ,  Geneva R. Davis ,  Gregory C. Holtz ,  Martin A. Gleeson ,  Bradley D. Howard

    IPC分类号： C07K14/65 ,  C12N15/81 ,  C12N15/18 ,  C12N1/19

    CPC分类号： C12N15/815 ,  C07K14/65 ,  C07K2319/02

    摘要： Insulin-like growth factor-1 (IGF-1), a naturally occurring, relatively short, single chain polypeptide, is prepared by growing methylotrophic yeast transformants containing in their genome at least one copy of DNA encoding IGF-1, in operational linkage with DNA encoding a signal sequence, which is effective for directing secretion of proteins from the host cells and which also includes the proteolytic processing site lys-arg and may include one or more glu-ala sequences. In preferred embodiments the signal sequence is the S. cerevisiae alpha mating factor pre-pro sequence. Expression of both the DNA encoding IGF-1 and the pre-pro signal sequence is regulated by a promoter region derived from a methanol responsive gene of a methylotrophic yeast. DNA constructs and recombinant methylotrophic yeast strains used for the expression and secretion of IGF-1 are also provided. For preferred embodiments, protease deficient Pichia pastoris strains are provided.

    摘要翻译： 胰岛素样生长因子-1（IGF-1）是一种天然存在的相对较短的单链多肽，通过生长含有其基因组的含有编码IGF-1的DNA的至少一个拷贝的甲基营养酵母转化体，与 编码信号序列的DNA，其对于引导来自宿主细胞的蛋白质的分泌是有效的，并且还包括蛋白水解加工位点lys-arg，并且可以包括一个或多个葡糖醛酸序列。 在优选实施方案中，信号序列是酿酒酵母α交配因子前序序列。 编码IGF-1和前一信号序列的DNA的表达由来自甲基营养酵母的甲醇应答基因的启动子区域调节。 还提供了用于IGF-1的表达和分泌的DNA构建体和重组甲基营养酵母菌株。 对于优选的实施方案，提供了蛋白酶缺乏的巴斯德毕赤酵母菌株。