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1.发明申请公开(公告)号：US20120244595A1
公开(公告)日：2012-09-27
申请号：US13059956
申请日：2009-08-19
CPC分类号： C12N9/14 , C07K2319/01 , C07K2319/035 , C12N9/2462 , C12Y302/01017
摘要： The present invention relates to recombinant polypeptides having the activity of binding and lysing of bacteria, comprising at least one enzymatically active domain and at least two bacterial cell binding domains. The present invention further relates to recombinant polypeptide having the activity of binding bacteria, comprising at least two bacterial cell binding domain. Further the present inventions relates to nucleic acid molecules comprising a nucleotide sequence encoding the recombinant polypeptides, vectors and host cells.
摘要翻译： 本发明涉及具有结合和裂解细菌活性的重组多肽，其包含至少一个酶活性结构域和至少两个细菌细胞结合结构域。 本发明还涉及具有结合细菌活性的重组多肽，其包含至少两个细菌细胞结合结构域。 此外，本发明涉及包含编码重组多肽，载体和宿主细胞的核苷酸序列的核酸分子。
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2.发明申请公开(公告)号：US20120171188A1
公开(公告)日：2012-07-05
申请号：US13251896
申请日：2011-10-03
CPC分类号： G01N33/56911 , A01N63/02 , C07K2319/01 , C12N9/1048 , C12N9/48 , C12N9/80 , C12N9/88
摘要： The present invention relates to recombinant polypeptides having the activity of binding and lysing of bacteria, comprising at least one enzymatically active domain and at least two bacterial cell binding domains. The present invention further relates to recombinant polypeptide having the activity of binding bacteria, comprising at least two bacterial cell binding domain. Further the present inventions relates to nucleic acid molecules comprising a nucleotide sequence encoding the recombinant polypeptides, vectors and host cells.
摘要翻译： 本发明涉及具有结合和裂解细菌活性的重组多肽，其包含至少一个酶活性结构域和至少两个细菌细胞结合结构域。 本发明还涉及具有结合细菌活性的重组多肽，其包含至少两个细菌细胞结合结构域。 此外，本发明涉及包含编码重组多肽，载体和宿主细胞的核苷酸序列的核酸分子。
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公开(公告)号：US20140079727A1
公开(公告)日：2014-03-20
申请号：US13055646
申请日：2009-07-24
CPC分类号： C12N9/2402 , A01N63/00 , A23B4/20 , A23L3/3526 , A23L3/3571 , A61K38/47 , A61K45/06 , C12N9/2462
摘要： The present invention relates to a polypeptide with an amino acid sequence according to SEQ ID NO:1. The present invention further relates to the nucleic acid molecules comprising a nucleotide sequence coding for the polypeptide, vectors comprising the nucleic acid molecules, and host cells for the expression of the polypeptides. In addition, the present invention relates to the use of the polypeptide as a human medical, veterinary medical or diagnostic substance, as an antimicrobial substance in food, in cosmetics, as disinfecting agent or in the environmental field.
摘要翻译： 本发明涉及具有根据SEQ ID NO：1的氨基酸序列的多肽。 本发明还涉及包含编码多肽的核苷酸序列，包含核酸分子的载体和用于表达多肽的宿主细胞的核酸分子。 此外，本发明涉及多肽作为人类医学，兽医医学或诊断物质，作为食品中的抗微生物物质，在化妆品中，作为消毒剂或在环境领域中的用途。
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4.发明申请Staphylococcus haemolyticus Prophage PhiSH2 Endolysin is Lytic for Staphylococcus aureus 有权溶血葡萄球菌菌PhiSH2内溶素是金黄色葡萄球菌的裂液公开(公告)号：US20140065127A1
公开(公告)日：2014-03-06
申请号：US13605200
申请日：2012-09-06
IPC分类号： C12N9/24
CPC分类号： C12N9/2402 , C12N9/2462 , C12Y302/01017
摘要： Methicillin-resistant (MRSA) and multi-drug resistant strains of Staphylococcus aureus are becoming increasingly prevalent in both human and veterinary clinics. S. aureus-causing bovine mastitis yields high annual losses to the dairy industry. Treatment of mastitis by broad range antibiotics is often not successful and may contribute to development of antibiotic resistance. Bacteriophage endolysins are a promising new source of antimicrobials. The endolysin of prophage φSH2 of Staphylococcus haemolyticus strain JCSC1435 (φSH2 lysin) shows lytic activity against live staphylococcal cells. Deletion constructs were tested in zymograms and turbidity reduction assays to evaluate the contribution of each functional module to lysis. The CHAP domain exhibited three-fold higher activity than the full length protein. Activity was further enhanced in the presence of bivalent calcium ions. The full length enzyme and the CHAP domain showed activity against multiple staphylococcal strains, including MRSA strains, mastitis isolates, and coagulase negative staphylococcal (CoNS) strains.
摘要翻译： 甲氧西林耐药（MRSA）和金黄色葡萄球菌多药耐药菌株在人类和兽医诊所中越来越普遍。 金黄色葡萄球菌引起的牛乳腺炎对乳制品行业的年度损失高。 通过广泛的抗生素治疗乳腺炎通常不成功，可能有助于抗生素耐药性的发展。 噬菌体内溶素是抗菌药物的有前途的新来源。 溶血葡萄球菌菌株JCSC1435（phiSH2溶素）的前噬菌体phiSH2的细胞内溶素显示对活葡萄球菌细胞的裂解活性。 在酶谱和浊度降低测定中测试缺失构建体，以评估每个功能模块对裂解的贡献。 CHAP结构域表现出比全长蛋白高三倍的活性。 在二价钙离子存在下，活性进一步提高。 全长酶和CHAP结构域显示了对多种葡萄球菌菌株的活性，包括MRSA菌株，乳腺炎分离株和凝固酶阴性葡萄球菌（CoNS）菌株。
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5.发明授权Staphylococcus haemolyticus prophage φSH2 endolysin is lytic for Staphylococcus aureus 有权溶血葡萄球菌原噬菌体SH2细胞内溶素溶解金黄色葡萄球菌公开(公告)号：US08980614B2
公开(公告)日：2015-03-17
申请号：US13605200
申请日：2012-09-06
CPC分类号： C12N9/2402 , C12N9/2462 , C12Y302/01017
摘要： Methicillin-resistant (MRSA) and multi-drug resistant strains of Staphylococcus aureus are becoming increasingly prevalent in both human and veterinary clinics. S. aureus-causing bovine mastitis yields high annual losses to the dairy industry. Treatment of mastitis by broad range antibiotics is often not successful and may contribute to development of antibiotic resistance. Bacteriophage endolysins are a promising new source of antimicrobials. The endolysin of prophage φSH2 of Staphylococcus haemolyticus strain JCSC1435 (φSH2 lysin) shows lytic activity against live staphylococcal cells. Deletion constructs were tested in zymograms and turbidity reduction assays to evaluate the contribution of each functional module to lysis. The CHAP domain exhibited three-fold higher activity than the full length protein. Activity was further enhanced in the presence of bivalent calcium ions. The full length enzyme and the CHAP domain showed activity against multiple staphylococcal strains, including MRSA strains, mastitis isolates, and coagulase negative staphylococcal (CoNS) strains.
摘要翻译： 甲氧西林耐药（MRSA）和金黄色葡萄球菌多药耐药菌株在人类和兽医诊所中越来越普遍。 金黄色葡萄球菌引起的牛乳腺炎对乳制品行业的年度损失高。 通过广泛的抗生素治疗乳腺炎通常不成功，可能有助于抗生素耐药性的发展。 噬菌体内溶素是抗菌药物的有前途的新来源。 溶血葡萄球菌菌株JCSC1435（＆phgr。SH2溶素）的原噬菌体SH2的细胞内溶素显示对活葡萄球菌细胞的裂解活性。 在酶谱和浊度降低测定中测试缺失构建体，以评估每个功能模块对裂解的贡献。 CHAP结构域表现出比全长蛋白高三倍的活性。 在二价钙离子存在下，活性进一步提高。 全长酶和CHAP结构域显示了对多种葡萄球菌菌株的活性，包括MRSA菌株，乳腺炎分离株和凝固酶阴性葡萄球菌（CoNS）菌株。
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