公开(公告)号：US08315820B2

公开(公告)日：2012-11-20

申请号：US12705214

申请日：2010-02-12

申请人： Masahiro Sasaki ,  Toshiyuki Kamo ,  Yoko Ohashi

发明人： Masahiro Sasaki ,  Toshiyuki Kamo ,  Yoko Ohashi

IPC分类号： G01N31/00

CPC分类号： G01N31/164

摘要： A potentiometric titration method including calculating a titration curve and a differential curve from measured values; computing a straight line (D) connecting the two measured values on the titration curve which are present adjacent to a tentative terminal point (P); computing a first estimation line (B1) and a second estimation line (B2) by a least squares method based on a set of the measured values (d1), (d2) and (d3) and a set of the measured values (d4), (d5) and (d6), respectively, on the titration curve, which are respectively spaced by a predetermined level apart from the tentative terminal point (P) by using the number of decision points (n) as an index where the number of decision points (n) represents the number of measured values on the previously set titration curve; computing an intermediate line (C) between the first estimation line (B1) and the second estimation line (B2); and computing an intersection point (A) of the intermediate line (C) and the straight line (D) to read out a value of the intersection point on an abscissa axis as a terminal point (a) of the titration.

摘要翻译： 电位滴定法，包括从测量值计算滴定曲线和微分曲线; 计算连接临时终点（P）附近的滴定曲线上的两个测量值的直线（D）; 基于一组测量值（d1），（d2）和（d3）和一组测量值（d4），通过最小二乘法计算第一估计线（B1）和第二估计线（B2） ，（d5）和（d6）分别通过使用决定点（n）的数量作为指标的数量，分别与临时终点（P）间隔预定水平的滴定曲线 决策点（n）表示先前设定的滴定曲线上的测量值的数量; 计算第一估计线（B1）和第二估计线（B2）之间的中间线（C）; 并计算中间线（C）和直线（D）的交点（A），以读出横坐标轴上的交点的值作为滴定的终点（a）。

公开(公告)号：US20100191479A1

公开(公告)日：2010-07-29

申请号：US12705214

申请日：2010-02-12

申请人： Masahiro SASAKI ,  Toshiyuki Kamo ,  Yoko Ohashi

发明人： Masahiro SASAKI ,  Toshiyuki Kamo ,  Yoko Ohashi

IPC分类号： G01N31/22 ,  G06F19/00

CPC分类号： G01N31/164

摘要： The present invention relates to a potentiometric titration method comprising assuming a titration curve and a differential curve from measured values; computing a straight line (D) connecting the two measured values on the titration curve which are present adjacent to a tentative terminal point (P); computing a first estimation line (B1) and a second estimation line (B2) by a least squares method based on a set of the measured values (d1), (d2) and (d3) and a set of the measured values (d4), (d5) and (d6), respectively, on the titration curve, which are respectively spaced by a predetermined level apart from the tentative terminal point (P) by using the number of decision points (n) as an index wherein the number of decision points (n) represents the number of measured values on the previously set titration curve; computing an intermediate line (C) between the first estimation line (B1) and the second estimation line (B2); and computing an intersection point (A) of the intermediate line (C) and the straight line (D) to read out a value of the intersection point on an abscissa axis as a terminal point (a) of the titration.

摘要翻译： 本发明涉及一种电位滴定法，其包括从测量值中提取滴定曲线和差示曲线; 计算连接临时终点（P）附近的滴定曲线上的两个测量值的直线（D）; 基于一组测量值（d1），（d2）和（d3）和一组测量值（d4），通过最小二乘法计算第一估计线（B1）和第二估计线（B2） ，（d5）和（d6）分别通过使用决定点（n）的数量作为指标的数量，分别与临时终点（P）间隔预定水平的滴定曲线 决策点（n）表示先前设定的滴定曲线上的测量值的数量; 计算第一估计线（B1）和第二估计线（B2）之间的中间线（C）; 并计算中间线（C）和直线（D）的交点（A），以读出横坐标轴上的交点的值作为滴定的终点（a）。

公开(公告)号：US20100227316A1

公开(公告)日：2010-09-09

申请号：US12159198

申请日：2006-12-26

申请人： Hirobumi Suzuki ,  Yoko Ohashi ,  Chiaki Sato ,  Morinao Fukuoka ,  Akihiro Namba

发明人： Hirobumi Suzuki ,  Yoko Ohashi ,  Chiaki Sato ,  Morinao Fukuoka ,  Akihiro Namba

IPC分类号： C12Q1/68 ,  C12M1/34

CPC分类号： G01N21/6458 ,  G02B21/0088

摘要： There is provided a novel luminescence measuring apparatus that enables measuring a luminescence amount or a luminescence intensity from plural living biological samples, such as tissues, cells, and biological individuals, into which a gene expressing a luminescent protein has been introduced, individually for the respective biological samples. The inventive luminescence measuring apparatus comprises an image acquisition portion that acquires a luminescence image of biological samples; a measurement region determination portion that determines at least one measurement region in the luminescence image; and a luminescence measurement portion that measures the luminescence amount or luminescence intensity in the determined measurement region; thereby measuring the luminescence amount or luminescence intensity individually by the biological sample(s) included in the measurement region. Irrespective of an efficiency of transfer of a luminescent protein gene into a cell, etc., it becomes possible to execute a luminescence measurement successfully while observing only biological samples having a gene expression.

摘要翻译： 提供了一种新颖的发光测量装置，其能够分别测量来自已经引入了表达发光蛋白质的基因的多个生物体生物样品（例如组织，细胞和生物个体）的发光量或发光强度 生物样品。 本发明的发光测量装置包括获取生物样品的发光图像的图像获取部分; 确定所述发光图像中的至少一个测量区域的测量区域确定部分; 以及测量所测定的测定区域的发光量或发光强度的发光测定部， 从而通过测量区域中包含的生物样品分别测量发光量或发光强度。 无论将发光蛋白质基因转移到细胞等中的效率如何，可以在仅观察具有基因表达的生物样品的同时成功地进行发光测定。

公开(公告)号：US08280142B2

公开(公告)日：2012-10-02

申请号：US13164208

申请日：2011-06-20

申请人： Hirobumi Suzuki ,  Yoko Ohashi ,  Kenji Kawasaki ,  Kiyotsugu Kojima ,  Kenichi Koyama ,  Akihiko Yoshikawa

发明人： Hirobumi Suzuki ,  Yoko Ohashi ,  Kenji Kawasaki ,  Kiyotsugu Kojima ,  Kenichi Koyama ,  Akihiko Yoshikawa

IPC分类号： G06K9/00

CPC分类号： G01N21/6428 ,  G01N21/6458 ,  G01N2021/6482 ,  G02B21/16 ,  G02B21/365

摘要： An object of the present invention is to provide a predetermined site luminescence measuring method and a predetermined site luminescence measuring apparatus, which allow for determining whether, when the luminescence from the predetermined site in live samples is measured, a photoprotein is localized at the predetermined site in the same ones as the samples. An predetermined site luminescence measuring apparatus 100 in the present invention is comprised of: a the sample 102 into which a fused fusion gene is introduced, the fusion gene being obtained by fusing a fluorescence-related gene that expresses a fluorescence protein in addition to a targeting base sequence and a luminescence-related gene; a container 103 for storing the sample 102, a stage 104 on which the container 103 is arranged; a luminescent image capturing unit 106 which captures a luminescent image of the sample 102 (the objective lens 106a to the CCD camera 106c, and the imaging lens 106f); a fluorescent image capturing unit 108 which captures a fluorescent image of the sample 102 (the objective lens 108a to shutter 108f); and an the information communication terminal 110.

摘要翻译： 本发明的目的是提供一种预定的现场发光测量方法和预定的现场发光测量装置，其允许确定当测量活样品中来自预定部位的发光时，光蛋白定位在预定位置 与样品相同。 本发明的预定位点发光测定装置100包括：融合融合基因导入到其中的样品102，所述融合基因除了靶向外，还融合表达荧光蛋白的荧光相关基因 碱基序列和发光相关基因; 用于存储样品102的容器103，设置容器103的载物台104; 捕获样品102（物镜106a到CCD照相机106c和成像透镜106f）的发光图像的发光图像捕获单元106。 捕获样品102（物镜108a至快门108f）的荧光图像的荧光图像捕获单元108; 和信息通信终端110。

公开(公告)号：US07986824B2

公开(公告)日：2011-07-26

申请号：US11887468

申请日：2006-03-30

申请人： Hirobumi Suzuki ,  Yoko Ohashi ,  Kenji Kawasaki ,  Kiyotsugu Kojima ,  Kenichi Koyama ,  Akihiko Yoshikawa

发明人： Hirobumi Suzuki ,  Yoko Ohashi ,  Kenji Kawasaki ,  Kiyotsugu Kojima ,  Kenichi Koyama ,  Akihiko Yoshikawa

IPC分类号： G06K9/00

CPC分类号： G01N21/6428 ,  G01N21/6458 ,  G01N2021/6482 ,  G02B21/16 ,  G02B21/365

摘要： An object of the present invention is to provide a predetermined site luminescence measuring method and a predetermined site luminescence measuring apparatus, which allow for determining whether, when the luminescence from the predetermined site in live samples is measured, a photoprotein is localized at the predetermined site in the same ones as the samples. An predetermined site luminescence measuring apparatus 100 in the present invention is comprised of: a the sample 102 into which a fused fusion gene is introduced, the fusion gene being obtained by fusing a fluorescence-related gene that expresses a fluorescence protein in addition to a targeting base sequence and a luminescence-related gene; a container 103 for storing the sample 102, a stage 104 on which the container 103 is arranged; a luminescent image capturing unit 106 which captures a luminescent image of the sample 102 (the objective lens 106a to the CCD camera 106c, and the imaging lens 106f); a fluorescent image capturing unit 108 which captures a fluorescent image of the sample 102 (the objective lens 108a to shutter 108f); and an the information communication terminal 110.

摘要翻译： 本发明的目的是提供一种预定的现场发光测量方法和预定的现场发光测量装置，其允许确定当测量活样品中来自预定部位的发光时，光蛋白定位在预定位置 与样品相同。 本发明的预定位点发光测定装置100包括：融合融合基因导入到其中的样品102，所述融合基因除了靶向外，还融合表达荧光蛋白的荧光相关基因 碱基序列和发光相关基因; 用于存储样品102的容器103，设置容器103的载物台104; 捕获样品102（物镜106a到CCD照相机106c和成像透镜106f）的发光图像的发光图像捕获单元106。 捕获样品102（物镜108a至快门108f）的荧光图像的荧光图像捕获单元108; 和信息通信终端110。

公开(公告)号：US06956229B2

公开(公告)日：2005-10-18

申请号：US10724242

申请日：2003-11-28

申请人： Shinichi Dosaka ,  Seiji Kondo ,  Sachiko Karaki ,  Yoko Ohashi

发明人： Shinichi Dosaka ,  Seiji Kondo ,  Sachiko Karaki ,  Yoko Ohashi

IPC分类号： G01N21/64 ,  G01N33/53 ,  G01N33/566 ,  G01N37/00 ,  G01N15/06

CPC分类号： G01N21/6452 ,  G01N21/6456 ,  G01N2021/6439 ,  G01N2021/6478

摘要： A fluorescence reader of the present invention detects fluorescence from a sample present on a carrier or in a solution, and the fluorescence reader includes a light source which radiates parallel light, a projection lens which converges the light from the light source, an objective lens which irradiates the sample with the light converged in a rear-side focal position, an image forming lens which forms fluorescence emitted from the sample and passed through the objective lens into an image, a light receiving pinhole disposed in an image forming position of the image forming lens, and detector which detects the fluorescence passed through the light receiving pinhole.

摘要翻译： 本发明的荧光读取器从存在于载体或溶液中的样品检测荧光，并且荧光读取器包括辐射平行光的光源，会聚来自光源的光的投影透镜，物镜 用会聚在后侧聚焦位置的光照射样品，形成从样品发出的荧光并透过物镜的图像形成透镜成为图像，设置在图像形成位置的图像形成位置的受光针孔 透镜和检测器，其检测通过光接收针孔的荧光。

公开(公告)号：US20090274360A1

公开(公告)日：2009-11-05

申请号：US11887468

申请日：2006-03-30

申请人： Hirobumi Suzuki ,  Yoko Ohashi ,  Kenji Kawasaki ,  Kiyotsugu Kojima ,  Kenichi Koyama ,  Akihiko Yoshikawa

发明人： Hirobumi Suzuki ,  Yoko Ohashi ,  Kenji Kawasaki ,  Kiyotsugu Kojima ,  Kenichi Koyama ,  Akihiko Yoshikawa

IPC分类号： G06T7/00 ,  G06K9/20

CPC分类号： G01N21/6428 ,  G01N21/6458 ,  G01N2021/6482 ,  G02B21/16 ,  G02B21/365

摘要： An object of the present invention is to provide a predetermined site luminescence measuring method and a predetermined site luminescence measuring apparatus, which allow for determining whether, when the luminescence from the predetermined site in live samples is measured, a photoprotein is localized at the predetermined site in the same ones as the samples. An predetermined site luminescence measuring apparatus 100 in the present invention is comprised of: a the sample 102 into which a fused fusion gene is introduced, the fusion gene being obtained by fusing a fluorescence-related gene that expresses a fluorescence protein in addition to a targeting base sequence and a luminescence-related gene; a container 103 for storing the sample 102, a stage 104 on which the container 103 is arranged; a luminescent image capturing unit 106 which captures a luminescent image of the sample 102 (the objective lens 106a to the CCD camera 106c, and the imaging lens 106f); a fluorescent image capturing unit 108 which captures a fluorescent image of the sample 102 (the objective lens 108a to shutter 108f); and an the information communication terminal 110.

摘要翻译： 本发明的目的是提供一种预定的现场发光测量方法和预定的现场发光测量装置，其允许确定当测量活样品中来自预定部位的发光时，光蛋白定位在预定位置 与样品相同。 本发明的预定位点发光测定装置100包括：融合融合基因导入到其中的样品102，所述融合基因除了靶向外，还融合表达荧光蛋白的荧光相关基因 碱基序列和发光相关基因; 用于存储样品102的容器103，设置容器103的载物台104; 捕获样品102（物镜106a到CCD照相机106c和成像透镜106f）的发光图像的发光图像捕获单元106。 捕获样品102（物镜108a至快门108f）的荧光图像的荧光图像捕获单元108; 和信息通信终端110。