公开(公告)号：US06543803B1

公开(公告)日：2003-04-08

申请号：US09616435

申请日：2000-07-14

申请人： Masami Harada ,  Yosuke Higashi

发明人： Masami Harada ,  Yosuke Higashi

IPC分类号： B60R2116

CPC分类号： B60R21/217 ,  B60R21/207 ,  B60R21/26

摘要： The invention provides an air bag apparatus which can securely prevent gas, generated from an inflator, from leaking from an insertion port of an air bag provided in a mounting base portion of the air bag. The air bag apparatus has a gas generating inflator (2) for expanding and developing an air bag (1) assembled in an inner portion from an insertion port (1c) provided in a mounting base portion (1b) of the air bag (1). The inflator (2) is positioned inside the air bag (1). An internal contact fabric (4) for closing the insertion port (1c) of the air bag (1) from the inner side is provided for preventing a gas leakage, and an external contact fabric (5) is provided for reinforcing the bag mounting base portion (1b).

摘要翻译： 本发明提供一种气囊装置，其能够可靠地防止从充气机产生的气体从设置在气囊的安装基部的气囊的插入口泄漏。 气囊装置具有用于使从气囊（1）的安装基部（1b）上的插入口（1c）组装在内部的气囊（1）膨胀和显影的气体发生用充气机（2） 。 充气机（2）位于气囊（1）的内部。 提供用于从内侧封闭气囊（1）的插入口（1c）的内部接触织物（4），用于防止气体泄漏，并且设置外部接触织物（5）以加强袋安装基座 （1b）。

公开(公告)号：US06357789B1

公开(公告)日：2002-03-19

申请号：US09557671

申请日：2000-04-25

申请人： Masami Harada ,  Yosuke Higashi

发明人： Masami Harada ,  Yosuke Higashi

IPC分类号： B60R2122

CPC分类号： B60R21/207 ,  B60R2021/161

摘要： In a seat with a side air bag system, of two stay cloths (9, 10) continuously extending from a sewing portion corresponding to a breaking portion (8, 80) of a trim cover (7), one stay cloth (9) is taken into a cavity (3) from an opening edge of a back pad (2) so as to fasten a take-in end within the cavity, the other stay cloth (10) is rolled in a rear side of the back pad (2) from a front side of an air bag system (1) so as to fasten a roll-in end in the rear side of the back pad, and the air bag system is wrapped by the two stay cloths. Accordingly, each of the stay cloths (9, 10) can be easily assembled and the air bag system (1) can be directly wrapped by the two stay cloths so as to efficiently concentrate an inflating pressure of the air bag on the sewing portion corresponding to the breaking portion (8, 80) of the trim cover (7), thereby quickly inflating and developing the air bag.

摘要翻译： 在具有侧气囊系统的座椅中，从对应于装饰罩（7）的断裂部分（8,80）的缝合部分连续延伸的两个支撑布（9,10），一个支撑布（9） 从背垫（2）的开口边缘被吸入空腔（3）中，以便将吸入端紧固在空腔内，另一支撑布（10）在背垫（2）的后侧滚动 ）从气囊系统（1）的前侧开始，以便将后垫的后侧的卷入端紧固，并且气囊系统被两个支撑布包裹。 因此，可以容易地组装每个支撑布（9,10），并且气囊系统（1）可以由两个支撑布直接包裹，以便有效地将气囊的膨胀压力集中在对应的缝合部分上 到装饰罩（7）的断开部分（8,80），从而使气囊迅速膨胀和显影。

公开(公告)号：US06439597B1

公开(公告)日：2002-08-27

申请号：US09557362

申请日：2000-04-25

申请人： Masami Harada ,  Yosuke Higashi ,  Jinichi Tanabe

发明人： Masami Harada ,  Yosuke Higashi ,  Jinichi Tanabe

IPC分类号： B60R2120

CPC分类号： B60R21/207 ,  B60R21/215

摘要： In a seat with a side air bag system, of two stay clothes (9, 10) continuously extending from a sewing portion corresponding to a breaking portion (8, 80) of a trim cover (7), one stay cloth (9) is taken into a cavity (3) from an opening edge of a back pad (2), the other stay cloth (10) is taken into the cavity (3) of the back pad (2) from a front side of an air bag system (1) so as to wrap the air bag system (1) with two stay clothes (9, 10), the stay clothes (9, 10) are connected and supported by wear plates (11, 12, 110) gripping and fixing a take-in end of each of the stay clothes (9, 10) between the air bag system (1) and the side plate (6). Accordingly, each of the stay clothes (9, 10) can be easily assembled and the air bag system (1) can be directly wrapped with two stay clothes so as to efficiently concentrate an inflating pressure of the air bag on the sewing portion corresponding to the breaking portion (8, 80) of the trim cover (7), thereby quickly inflating and developing the air bag.

摘要翻译： 在具有侧面安全气囊系统的座椅中，从对应于装饰罩（7）的断裂部分（8,80）的缝合部分连续延伸的两个停留衣服（9,10），一个停留布（9） 从背垫（2）的开口边缘吸入空腔（3）中，另一个支撑布（10）从安全气囊系统的前侧被吸入背垫（2）的空腔（3） （1），以使两个衣服（9,10）包裹所述气囊系统（1），所述停留衣物（9,10）由耐磨板（11,12,110）连接并支撑，所述耐磨板（11,12,110）夹持并固定 在气囊系统（1）和侧板（6）之间的每个衣服（9,10）的入口端。 因此，能够容易地组装各衣服（9,10），并且可以将两个衣服直接包裹在一起，从而有效地将安全气囊的充气压力集中在对应于 装饰罩（7）的断开部分（8,80），从而使气囊迅速膨胀和显影。

公开(公告)号：US5968789A

公开(公告)日：1999-10-19

申请号：US808218

申请日：1997-02-28

申请人： Yukihito Yukimune ,  Yasuhiro Hara ,  Yosuke Higashi ,  Naoto Ohnishi ,  Homare Tabata ,  Chuzo Suga ,  Kouichi Matsubara

发明人： Yukihito Yukimune ,  Yasuhiro Hara ,  Yosuke Higashi ,  Naoto Ohnishi ,  Homare Tabata ,  Chuzo Suga ,  Kouichi Matsubara

IPC分类号： C12N5/00 ,  C12N5/04 ,  C12P17/02

CPC分类号： C12N5/04 ,  C12N5/0025 ,  C12P17/02

摘要： This invention relates to a method of producing a taxane-type diterpene(s) wherein tissues or cells of a plant which produces taxane-type diterpene(s) is cultured in the presence of at least one selected from the group consisting of jasmonic acids, compounds containing a heavy metal, complex ions containing a heavy metal, heavy metal ions, amines and antiethylene agents, a method of producing a taxane-type diterpene wherein the tissues or the cells of the plant are cultured by controlling the oxygen concentration in a gas phase in a culture vessel to less than the oxygen concentration in the atmosphere from the initial stage of the culture, or by controlling the dissolved oxygen concentration in a fluid medium which is in contact with the tissue or the cell to less than the saturated dissolved oxygen concentration at that temperature from the initial stage of the culture, a method of producing a taxane-type diterpene wherein the tissue or the cell of the plant is cultured in a culture vessel, while oxygenic gas containing 0.03-10% of carbon dioxide is used as aeration gas to be introduced to the vessel, and a method of obtaining highly productive cultured cells for the taxane-type diterpene wherein cultured cells of the plant which produces the taxane-type diterpene are separated into a plurality of layers according to the difference in their specific gravities, and the cells contained in at least one layer are cultured, then highly productive cultured cells for the taxane-type diterpene are selected from among those cultured cells.The present invention allows industrial production of a taxane-type diterpene such as taxol which is useful as a therapeutic agent for cancer.

摘要翻译： 本发明涉及一种生产紫杉烷类双萜的方法，其中在选自茉莉酸，茉莉酮酸，缬氨酸，缬氨酸， 含有重金属的化合物，含有重金属的配合离子，重金属离子，胺类和抗乙烯剂等，制备紫杉烷类双萜的方法，其中通过控制气体中的氧浓度来培养植物的组织或细胞 培养容器中的相位低于培养初期的大气中的氧浓度，或通过将与组织或细胞接触的流体培养基中的溶解氧浓度控制为小于饱和溶解氧 从培养的初始阶段开始的温度下的浓度，制备紫杉烷类双萜的方法，其中在培养物中培养植物的组织或细胞 容器，而含有0.03-10％二氧化碳的含氧气体用作引入容器的通气气体，以及获得紫杉烷类双萜高产培养细胞的方法，其中产生紫杉烷的植物培养细胞 型二萜根据其比重差异分离成多个层，培养至少一层所含的细胞，然后从培养的细胞中选出紫杉烷类双萜的高效培养细胞。 本发明允许工业生产可用作癌症治疗剂的紫杉烷类二萜类例如紫杉醇。

公开(公告)号：US08669093B2

公开(公告)日：2014-03-11

申请号：US10573813

申请日：2004-09-17

申请人： Mitsufumi Wade ,  Toshihiro Oikawa ,  Daisuke Mochizuki ,  Junko Tokuda ,  Miyuki Kawashima ,  Tadashi Araki ,  Reiko Abe ,  Hitoki Miyake ,  Hitoshi Takahashi ,  Hideki Sawai ,  Takashi Mimitsuka ,  Takashi Morishige ,  Yosuke Higashi

发明人： Mitsufumi Wade ,  Toshihiro Oikawa ,  Daisuke Mochizuki ,  Junko Tokuda ,  Miyuki Kawashima ,  Tadashi Araki ,  Reiko Abe ,  Hitoki Miyake ,  Hitoshi Takahashi ,  Hideki Sawai ,  Takashi Mimitsuka ,  Takashi Morishige ,  Yosuke Higashi

IPC分类号： C12N1/21 ,  C12P7/56 ,  C12N1/26

CPC分类号： C12P7/56

摘要： A method for producing D-lactic acid in high yield, and to provide a method for producing D-lactic acid with high selectivity, in which optical purity is high and a by-product organic acid is small. In one aspect, a microorganism, wherein activity of pyruvate formate-lyase (pfl) is inactivated or decreased, and further activity of Escherichia coli-derived NADH-dependent D-lactate dehydrogenase (ldhA) is enhanced, is cultured to efficiently produce D-lactic acid. With regard to a method for enhancing ldhA activity, by linking, on a genome, a gene encoding ldhA with a promoter of a gene which controls expression of a protein involved in a glycolytic pathway, a nucleic acid biosynthesis pathway or an amino acid biosynthesis pathway, suitable results are obtained compared to the method for enhancing expression of the gene using an expression vector. A microorganism in which a dld gene is substantially inactivated or decreased is cultured to produce high quality D-lactic acid with reduced concentration of pyruvic acid.

摘要翻译： 提供高产率D-乳酸的制造方法，提供高纯度的高纯度D-副乳酸和副产物有机酸的生产方法。 在一个方面，培养其中丙酮酸甲酸裂合酶（pfl）的活性失活或降低，并且进一步增强大肠杆菌来源的NADH依赖性D-乳酸脱氢酶（ldhA）的活性的微生物，以有效地产生D- 乳酸。 关于增强ldhA活性的方法，通过在基因组上连接编码ldhA的基因与控制糖酵解途径参与蛋白质的表达的基因的启动子，核酸生物合成途径或氨基酸生物合成途径 与使用表达载体增强基因表达的方法相比，获得了合适的结果。 培养其中dld基因基本上失活或减少的微生物被培养以产生具有降低的丙酮酸浓度的高质量D-乳酸。

公开(公告)号：US06357786B1

公开(公告)日：2002-03-19

申请号：US09634680

申请日：2000-08-08

申请人： Yosuke Higashi

发明人： Yosuke Higashi

IPC分类号： B60R2116

CPC分类号： B60R21/20 ,  B60R2021/26076

摘要： There is provided an air bag system in which leakage of gas generated by an inflator through an insertion opening formed at a mounting base portion of an air bag is prevented reliably. The air bag system has the air bag in which the inflator for generating gas for inflating and deploying the air bag is mounted through the insertion opening formed at the mounting base portion of said air bag. The air bag system has an applied cloth mounted to an outside of the air bag such that the insertion opening for the inflator is covered with the applied cloth by making one side of the applied cloth an open hole and fixing edges of three other sides of the applied cloth to an outside face of the mounting base portion.

摘要翻译： 提供一种气囊系统，其中可靠地防止由充气机产生的气体通过形成在安全气囊的安装基部的插入开口泄漏。 气囊系统具有气囊，通过形成在所述安全气囊的安装基部的插入口安装用于产生用于充气和展开气囊的气体的充气机。 安全气囊系统具有安装在气囊外部的涂抹布，使得用于布料的布料用打开的孔覆盖，使得充气机的插入开口被覆盖，并且固定着另外一侧的边缘 将布施加到安装基部的外表面。

公开(公告)号：US5637484A

公开(公告)日：1997-06-10

申请号：US491844

申请日：1995-07-14

申请人： Yukihito Yukimune ,  Yasuhiro Hara ,  Yosuke Higashi ,  Naoto Ohnishi ,  Homare Tabata ,  Chuzo Suga ,  Kouichi Matsubara

发明人： Yukihito Yukimune ,  Yasuhiro Hara ,  Yosuke Higashi ,  Naoto Ohnishi ,  Homare Tabata ,  Chuzo Suga ,  Kouichi Matsubara

IPC分类号： C12N5/00 ,  C12N5/04 ,  C12P17/02 ,  C12P1/00 ,  C12P15/00

CPC分类号： C12N5/04 ,  C12N5/0025 ,  C12P17/02

摘要： This invention relates to a method of producing a taxane-type diterpene(s) wherein tissues or cells of a plant which produces taxane-type diterpene(s) are cultured in the presence of at least one member selected from the group consisting of jasmonic acids, wherein the tissues or the cells of the plant are cultured by controlling the oxygen concentration in a gas phase in a culture vessel to less than the oxygen concentration in the atmosphere from the initial stage of the culture, or by controlling the dissolved oxygen concentration in a fluid medium which is in contact with the tissue or the cell to less than the saturated dissolved oxygen concentration at that temperature from the initial stage of the culture, while oxygenic gas containing 0.03-10% of carbon dioxide is used as aeration gas to be introduced to the vessel, and a method of obtaining highly productive cultured cells for the taxane-type diterpene wherein cultured cells of the plant which produces the taxane-type diterpene are separated into a plurality of layers according to the difference in their specific gravities, and the cells contained in at least one layer are cultured, then highly productive cultured cells for the taxane-type diterpene are selected from among those cultured cells. The present invention allows industrial production of a taxane-type diterpene such as taxol which is useful as a therapeutic agent for cancer.

摘要翻译： PCT No.PCT / JP94 / 01880 Sec。 371 1995年7月14日第 102（e）日期1995年7月14日PCT 1994年11月9日PCT PCT。 WO95 / 14103 PCT出版物 日期1995年5月26日本发明涉及一种生产紫杉烷型双萜的方法，其中在存在有至少一种选自下组的成员的情况下培养产生紫杉烷类双萜的植物的组织或细胞 由茉莉酸组成，其中通过将培养容器中的气相中的氧浓度控制为从培养初期的大气中的氧浓度来培养植物的组织或细胞，或者通过控制 在与培养初期接触的流体介质中的溶解氧浓度低于培养初期的该温度下的饱和溶解氧浓度，而使用含有0.03-10％二氧化碳的含氧气体作为 要引入容器的曝气气体，以及获得紫杉烷类双萜高产培养细胞的方法，其中产生分类单位的植物培养细胞 根据其比重差异将ne型双萜分离成多个层，并培养至少一层中所含的细胞，然后从培养细胞中选出紫杉烷类双萜的高效培养细胞 。 本发明允许工业生产可用作癌症治疗剂的紫杉烷类二萜类例如紫杉醇。

公开(公告)号：US20070065930A1

公开(公告)日：2007-03-22

申请号：US10573813

申请日：2004-09-17

申请人： Mitsufumi Wada ,  Toshihiro Oikawa ,  Daisuke Mochizuki ,  Junko Tokuda ,  Miyuki Kawashima ,  Tadashi Araki ,  Reiko Abe ,  Hitoki Miyake ,  Hitoshi Takahashi ,  Hideki Sawai ,  Takashi Mimitsuka ,  Takashi Morishige ,  Yosuke Higashi

发明人： Mitsufumi Wada ,  Toshihiro Oikawa ,  Daisuke Mochizuki ,  Junko Tokuda ,  Miyuki Kawashima ,  Tadashi Araki ,  Reiko Abe ,  Hitoki Miyake ,  Hitoshi Takahashi ,  Hideki Sawai ,  Takashi Mimitsuka ,  Takashi Morishige ,  Yosuke Higashi

IPC分类号： C12P7/56 ,  C12N1/21

CPC分类号： C12P7/56

摘要： A method for producing D-lactic acid in high yield, and to provide a method for producing D-lactic acid with high selectivity, in which optical purity is high and a by-product organic acid is small. In one aspect, a microorganism, wherein activity of pyruvate formate-lyase (pfl) is inactivated or decreased, and further activity of Escherichia coli-derived NADH-dependent D-lactate dehydrogenase (ldhA) is enhanced, is cultured to efficiently produce D-lactic acid. With regard to a method for enhancing ldhA activity, by linking, on a genome, a gene encoding ldhA with a promoter of a gene which controls expression of a protein involved in a glycolytic pathway, a nucleic acid biosynthesis pathway or an amino acid biosynthesis pathway, suitable results are obtained compared to the method for enhancing expression of the gene using an expression vector. A microorganism in which a dld gene is substantially inactivated or decreased is cultured to produce high quality D-lactic acid with reduced concentration of pyruvic acid.

摘要翻译： 提供高产率D-乳酸的制造方法，提供高纯度的高纯度D-副乳酸和副产物有机酸的生产方法。 在一个方面，培养其中丙酮酸甲酸裂合酶（pfl）的活性失活或降低，并且进一步增强大肠杆菌来源的NADH依赖性D-乳酸脱氢酶（ldhA）的活性的微生物，以有效地产生D- 乳酸。 关于增强ldhA活性的方法，通过在基因组上连接编码ldhA的基因与控制糖酵解途径参与蛋白质的表达的基因的启动子，核酸生物合成途径或氨基酸生物合成途径 与使用表达载体增强基因表达的方法相比，获得了合适的结果。 培养其中dld基因基本上失活或减少的微生物被培养以产生具有降低的丙酮酸浓度的高质量D-乳酸。

公开(公告)号：US06578866B2

公开(公告)日：2003-06-17

申请号：US10222143

申请日：2002-08-16

申请人： Yosuke Higashi

发明人： Yosuke Higashi

IPC分类号： B60R2116

CPC分类号： B60R21/217 ,  B60R21/207 ,  B60R21/26

摘要： The invention provides an air bag apparatus which can securely prevent gas generated from an inflator from leaking from an insertion port of the air bag provided in a mounting base portion of the air bag. The air bag apparatus has a gas generating inflator (2) for expanding and developing an air bag (1) assembled in an inner portion from an insertion port (1c) provided in a mounting base portion (1b) of the air bag (1), the inflator (2) is received inside within the air bag (1), an internal contact fabric (4) for closing the insertion port (1c) of the air bag (1) from the inner side is provided for preventing a gas leakage, and an external contact fabric (5) is provided for reinforcing the bag mounting base portion (1b).

摘要翻译： 本发明提供一种气囊装置，其能够可靠地防止从充气机产生的气体从设置在气囊的安装基部的气囊的插入口泄漏。 气囊装置具有用于使从气囊（1）的安装基部（1b）上的插入口（1c）组装在内部的气囊（1）膨胀和显影的气体发生用充气机（2） 充气器（2）被接纳在气囊（1）的内部，用于从内侧封闭气囊（1）的插入口（1c）的内部接触织物（4）用于防止气体泄漏 ，并且提供外部接触织物（5）以加强袋安装基部（1b）。

公开(公告)号：US06403343B2

公开(公告)日：2002-06-11

申请号：US09301075

申请日：1999-04-28

申请人： Yukihito Yukimune ,  Yasuhiro Hara ,  Yosuke Higashi ,  Naoto Ohnishi ,  Homare Tabata ,  Chuzo Suga ,  Kouichi Matsubara

发明人： Yukihito Yukimune ,  Yasuhiro Hara ,  Yosuke Higashi ,  Naoto Ohnishi ,  Homare Tabata ,  Chuzo Suga ,  Kouichi Matsubara

IPC分类号： C12P1702

CPC分类号： C12N5/04 ,  C12N5/0025 ,  C12P17/02

摘要： This invention relates to a method of producing a taxane-type diterpene(s) wherein tissues or cells of a plant which produces taxane-type diterpene(s) is cultured in the presence of at least one selected from the group consisting of jasmonic acids, compounds containing a heavy metal, complex ions containing a heavy metal, heavy metal ions, amines and antiethylene agents, a method of producing a taxane-type diterpene wherein the tissues or the cells of the plant are cultured by controlling the oxygen concentration in a gas phase in a culture vessel to less than the oxygen concentration in the atmosphere from the initial stage of the culture, or by controlling the dissolved oxygen concentration in a fluid medium which is in contact with the tissue or the cell to less than the saturated dissolved oxygen concentration at that temperature from the initial stage of the culture, a method of producing a taxane-type diterpene wherein the tissue or the cell of the plant is cultured in a culture vessel, while oxygenic gas containing 0.03-10 % of carbon dioxide is used as aeration gas to be introduced to the vessel, and a method of obtaining highly productive cultured cells for the taxane-type diterpene wherein cultured cells of the plant which produces the taxane-type diterpene are separated into a plurality of layers according to the difference in their specific gravities, and the cells contained in at least one layer are cultured, then highly productive cultured cells for the taxane-type diterpene are selected from among those cultured cells. The present invention allows industrial production of a taxane-type diterpene such as taxol which is useful as a therapeutic agent for cancer.

摘要翻译： 本发明涉及一种生产紫杉烷类双萜的方法，其中在选自茉莉酸，茉莉酮酸，缬氨酸，缬氨酸， 含有重金属的化合物，含有重金属的配合离子，重金属离子，胺类和抗乙烯剂等，制备紫杉烷类双萜的方法，其中通过控制气体中的氧浓度来培养植物的组织或细胞 培养容器中的相位低于培养初期的大气中的氧浓度，或通过将与组织或细胞接触的流体培养基中的溶解氧浓度控制为小于饱和溶解氧 从培养的初始阶段开始的温度下的浓度，制备紫杉烷类双萜的方法，其中在培养物中培养植物的组织或细胞 容器，而含有0.03-10％二氧化碳的含氧气体用作引入容器的通气气体，以及获得紫杉烷类双萜高产培养细胞的方法，其中产生紫杉烷的植物培养细胞 型二萜根据其比重差异分离成多个层，培养至少一层所含的细胞，然后从那些培养的细胞中选出紫杉烷类双萜的高效培养细胞。 本发明允许工业生产可用作癌症治疗剂的紫杉烷类二萜类例如紫杉醇。