公开(公告)号：US08822195B2

公开(公告)日：2014-09-02

申请号：US13375882

申请日：2010-06-02

申请人： Kenji Sawai ,  Kazumi Suda ,  Hideki Sawai ,  Katsushige Yamada ,  Junya Yamagishi

发明人： Kenji Sawai ,  Kazumi Suda ,  Hideki Sawai ,  Katsushige Yamada ,  Junya Yamagishi

IPC分类号： C12N1/20 ,  C12N15/00 ,  C12N9/04 ,  C12P7/56

CPC分类号： C12P7/56 ,  C12N9/0006

摘要： Highly productive D-lactic acid fermentation uses a transformant obtained by introducing into a host cell a polynucleotide encoding a polypeptide according to any one of the following (A) to (C) in such a manner that the polypeptide is expressed, which polypeptide has a D-lactate dehydrogenase activity higher than those of conventional polypeptides: (A) a polypeptide having the amino acid sequence shown in SEQ ID NO:1 or 2; (B) a polypeptide having the same amino acid sequence as shown in SEQ ID NO:1 or 2 except that one or several amino acids are substituted, deleted, inserted and/or added, which polypeptide has a D-lactate dehydrogenase activity; and (C) a polypeptide having an amino acid sequence which has a sequence identity of not less than 80% to the amino acid sequence shown in SEQ ID NO:1 or 2, which polypeptide has a D-lactate dehydrogenase activity.

摘要翻译： 高生产率D-乳酸发酵使用通过向宿主细胞中引入编码根据下列（A）至（C）中任一项所述的多肽的多核苷酸而获得的转化体，使得多肽表达， D-乳酸脱氢酶活性高于常规多肽的活性：（A）具有SEQ ID NO：1或2所示氨基酸序列的多肽; （B）具有与SEQ ID NO：1或2所示相同氨基酸序列的多肽，不同之处在于一个或几个氨基酸被取代，缺失，插入和/或添加，该多肽具有D-乳酸脱氢酶活性; 和（C）具有与SEQ ID NO：1或2所示的氨基酸序列具有不小于80％的序列同一性的氨基酸序列的多肽，该多肽具有D-乳酸脱氢酶活性。

公开(公告)号：US20140004576A1

公开(公告)日：2014-01-02

申请号：US13991929

申请日：2011-12-08

申请人： Nanami Sasaki ,  Takashi Mimitsuka ,  Hideki Sawai ,  Kenji Sawai

发明人： Nanami Sasaki ,  Takashi Mimitsuka ,  Hideki Sawai ,  Kenji Sawai

IPC分类号： C12P13/00

CPC分类号： C12P13/001 ,  C12N9/0006 ,  C12N9/88 ,  C12Y101/01003 ,  C12Y401/01018

摘要： A method of producing cadaverine is more efficient and at a higher yield than production methods by the conventional fermentation methods. The method includes culturing coryneform bacterium/bacteria having a resistance to a pH of 5.5 or less. Preferably, the coryneform bacterium/bacteria has/have lysine decarboxylase activity and, preferably, the coryneform bacterium/bacteria has/have homoserine auxotrophy and/or a resistance to S-(2-aminoethyl)-L-cysteine.

摘要翻译： 与通过常规发酵方法的生产方法相比，生产尸胺的方法更有效并且产量高。 该方法包括培养耐pH为5.5以下的棒状细菌/细菌。 优选地，棒状细菌/细菌具有/具有赖氨酸脱羧酶活性，优选棒状细菌/细菌具有高丝氨酸营养缺陷型和/或对S-（2-氨基乙基）-L-半胱氨酸的抗性。

公开(公告)号：US20130323800A1

公开(公告)日：2013-12-05

申请号：US13992081

申请日：2011-12-08

申请人： Nanami Sasaki ,  Takashi Mimitsuka ,  Hideki Sawai ,  Kenji Sawai

发明人： Nanami Sasaki ,  Takashi Mimitsuka ,  Hideki Sawai ,  Kenji Sawai

IPC分类号： C12P13/00

CPC分类号： C12P13/001 ,  C12Y401/01018

摘要： A method produces cadaverine more efficiently and at a higher yield than production methods by the conventional fermentation methods. The method includes culturing coryneform bacterium/bacteria having an ability to produce cadaverine and having a resistance to 2,2′-thiobis(ethylamine). Preferably, the coryneform bacterium/bacteria has/have lysine decarboxylase activity and, preferably, the coryneform bacterium/bacteria has/have homoserine auxotrophy and/or a resistance to S-(2-aminoethyl)-L-cysteine.

摘要翻译： 一种方法通过常规发酵方法比生产方法更有效地产生尸胺，并且产量高。 该方法包括培养具有产生尸胺的能力并具有对2,2'-硫代双（乙胺）的抗性的棒状细菌/细菌。 优选地，棒状细菌/细菌具有/具有赖氨酸脱羧酶活性，优选棒状细菌/细菌具有高丝氨酸营养缺陷型和/或对S-（2-氨基乙基）-L-半胱氨酸的抗性。

公开(公告)号：US08551745B2

公开(公告)日：2013-10-08

申请号：US12865934

申请日：2009-02-03

申请人： Nanami Sasaki ,  Ken Morita ,  Takashi Mimitsuka ,  Hideki Sawai ,  Katsushige Yamada

发明人： Nanami Sasaki ,  Ken Morita ,  Takashi Mimitsuka ,  Hideki Sawai ,  Katsushige Yamada

IPC分类号： C12P7/56

CPC分类号： C12P7/56

摘要： A method of producing lactic acid continuous fermentation including filtering a culture of polyploid yeast having a capacity to produce lactic acid through a porous membrane having an average pore size of not less than 0.01 μm and less than 1 μm, and recovering the product from the filtrate while the unfiltered liquid is retained in or returned to the culture and a fermentation feedstock is added to the culture.

摘要翻译： 一种生产乳酸连续发酵的方法，包括通过平均孔径不小于0.01μm和小于1μm的多孔膜过滤具有生产乳酸能力的多倍体酵母的培养物，并从滤液中回收产物 而未过滤的液体保留在或返回到培养物中，并且向培养物中加入发酵原料。

公开(公告)号：US20130095534A1

公开(公告)日：2013-04-18

申请号：US13580742

申请日：2011-02-22

申请人： Takashi Mimitsuka ,  Kazumi Suda ,  Hideki Sawai

发明人： Takashi Mimitsuka ,  Kazumi Suda ,  Hideki Sawai

IPC分类号： C12P13/00

CPC分类号： C12P13/001 ,  C07K2319/02 ,  C12N9/88

摘要： By a method for producing cadaverine by culturing a microorganism that extracellularly secretes lysine decarboxylase, by-production of lysine is suppressed, the yield of cadaverine relative to glucose consumption is improved compared to conventional production methods, and further, the load on the purification step in purification of cadaverine as a raw material for polyamide can be reduced.

摘要翻译： 通过培养细胞外分泌赖氨酸脱羧酶的微生物来制造尸胺的方法，抑制赖氨酸的副产生，与常规生产方法相比，尸胺相对于葡萄糖消耗的产率提高，此外，纯化步骤中的负荷 可以降低作为聚酰胺原料的尸胺的净化。

公开(公告)号：US08349597B2

公开(公告)日：2013-01-08

申请号：US12666690

申请日：2008-06-18

申请人： Masateru Ito ,  Shin-ichi Minegishi ,  Eri Shimizu ,  Kenji Sawai ,  Yohito Ito ,  Hideki Sawai ,  Katsushige Yamada

发明人： Masateru Ito ,  Shin-ichi Minegishi ,  Eri Shimizu ,  Kenji Sawai ,  Yohito Ito ,  Hideki Sawai ,  Katsushige Yamada

IPC分类号： C12P7/56

CPC分类号： C12P7/56 ,  B01D61/027 ,  B01D71/56 ,  B01D2311/06 ,  B01D2311/18 ,  B01D2311/2669

摘要： A method of producing lactic acid by separating lactic acid produced in a culture solution by microbial fermentation, comprising: a step (A) of filtering the culture solution through a nano-filtration membrane; and a step (B) of distilling a lactic-acid-containing solution produced in step (A) under a pressure of 1 Pa to atmospheric pressure at a temperature of 25 to 200° C. to recover lactic acid.

摘要翻译： 通过微生物发酵分离培养液中产生的乳酸来生产乳酸的方法，包括：通过纳滤膜过滤培养液的步骤（A）; 和在步骤（A）中制备的含乳酸的溶液在25℃至200℃的温度下在1Pa至大气压的压力下蒸馏以回收乳酸的步骤（B）。

公开(公告)号：US20120253082A1

公开(公告)日：2012-10-04

申请号：US13076053

申请日：2011-03-30

申请人： Izumi MORITA ,  Masateru Ito ,  Hideki Sawai ,  Shinichi Minegishi ,  Katsushige Yamada ,  Kenji Kawamura ,  Shinichi Yamane

发明人： Izumi MORITA ,  Masateru Ito ,  Hideki Sawai ,  Shinichi Minegishi ,  Katsushige Yamada ,  Kenji Kawamura ,  Shinichi Yamane

IPC分类号： C07C29/76

CPC分类号： C07C29/76 ,  C07C31/202 ,  C07C31/205 ,  C07C31/207 ,  C07C31/225

摘要： A method for producing a diol or triol, which has a step of removing impurities contained in a diol- or triol-containing solution, is provided. In the method, a diol- or triol-containing solution is filtered through a nanofiltration membrane having a polyamide-containing functional layer. The diol- or triol-containing solution is then collected from the permeate flow of the nanofiltration membrane.

摘要翻译： 提供了一种制备二醇或三醇的方法，其具有除去二醇或三醇溶液中所含杂质的步骤。 在该方法中，通过具有含聚酰胺功能层的纳滤膜过滤二醇或三醇溶液。 然后从纳滤膜的渗透流中收集含二醇或三醇的溶液。

公开(公告)号：US20110250637A1

公开(公告)日：2011-10-13

申请号：US13133788

申请日：2009-12-08

申请人： Hiroyuki Kurihara ,  Atsushi Minamino ,  Masateru Ito ,  Hideki Sawai ,  Masayuki Hanakawa ,  Shin-ichi Minegishi ,  Katsushige Yamada

发明人： Hiroyuki Kurihara ,  Atsushi Minamino ,  Masateru Ito ,  Hideki Sawai ,  Masayuki Hanakawa ,  Shin-ichi Minegishi ,  Katsushige Yamada

IPC分类号： C12P1/00 ,  C13K13/00

CPC分类号： C08B1/003 ,  B01D61/022 ,  B01D61/025 ,  B01D61/027 ,  C12N1/38 ,  C12P7/10 ,  C12P7/46 ,  C12P7/56 ,  C12P13/001 ,  C12P19/02 ,  C12P19/14 ,  C12P2203/00 ,  C13B20/165 ,  C13K1/02 ,  C13K1/04 ,  C13K13/00 ,  C13K13/002 ,  Y02E50/16

摘要： A sugar liquid containing only very small amounts of fermentation-inhibiting substances is produced by a method for producing a sugar liquid using a cellulose-containing biomass as a raw material, the method including: (1) a step of hydrolyzing a cellulose-containing biomass to produce an aqueous sugar solution; and (2) a step of filtering the obtained aqueous sugar solution through a nanofiltration membrane and/or reverse osmosis membrane to collect a purified sugar liquid from the feed side, while removing fermentation-inhibiting substances from the permeate side.

摘要翻译： 通过使用含纤维素的生物质作为原料的糖液的制造方法制造含有非常少量的发酵抑制物质的糖液，该方法包括：（1）水解含纤维素的生物质的步骤 以产生糖水溶液; 和（2）通过纳滤膜和/或反渗透膜过滤得到的糖水溶液的步骤，从进料侧收集纯化的糖液，同时从渗透侧除去发酵抑制物质。

公开(公告)号：US20090239274A1

公开(公告)日：2009-09-24

申请号：US12083315

申请日：2006-09-04

申请人： Hideki Sawai ,  Kenji Sawai ,  Tomonori Sonoki ,  Satoko Hatahira

发明人： Hideki Sawai ,  Kenji Sawai ,  Tomonori Sonoki ,  Satoko Hatahira

IPC分类号： C12P7/56 ,  C12N1/19

CPC分类号： C12N9/0006 ,  C12P7/56

摘要： Yeast includes an introduced gene coding a Homo sapiens- or frog-derived L-lactate dehydrogenase.It is possible to produce lactic acid, which has a variety of applications, efficiently and more cost-effectively by using the yeast and the method of producing lactic acid by using the yeast.

摘要翻译： 酵母包括编码智人或青蛙衍生的L-乳酸脱氢酶的引入基因。 通过使用酵母和通过使用酵母生产乳酸的方法，可以生产具有多种应用的乳酸，有效且更经济有效。

公开(公告)号：US09587253B2

公开(公告)日：2017-03-07

申请号：US12280197

申请日：2007-02-16

申请人： Hideki Sawai ,  Katsushige Yamada ,  Takashi Mimitsuka ,  Kenji Sawai ,  Tetsu Yonehara ,  Yohito Ito ,  Masahiro Henmi

发明人： Hideki Sawai ,  Katsushige Yamada ,  Takashi Mimitsuka ,  Kenji Sawai ,  Tetsu Yonehara ,  Yohito Ito ,  Masahiro Henmi

IPC分类号： C12P13/04 ,  C12P7/06 ,  C12M1/00 ,  C12M1/34 ,  C12P7/18 ,  C12P7/46 ,  C12P7/50 ,  C12P7/56 ,  C12P13/00 ,  C12P13/08 ,  C12P19/30

CPC分类号： C12P7/06 ,  C12M29/04 ,  C12M29/18 ,  C12M41/40 ,  C12M47/10 ,  C12P7/18 ,  C12P7/46 ,  C12P7/50 ,  C12P7/56 ,  C12P13/001 ,  C12P13/005 ,  C12P13/04 ,  C12P13/08 ,  C12P19/30 ,  Y02E50/17

摘要： The invention provides a method of producing a chemical product through continuous fermentation which includes filtering a culture of a microorganism or cultured cells with a separation membrane to recover a product from a filtrate and simultaneously retaining a nonfiltered fluid in, or refluxing it to, the culture, and adding fermentation materials to the culture, wherein a porous membrane having an average pore size of 0.01 μm or more to less than 1 μm is used as the separation membrane and the filtration is conducted with a transmembrane pressure difference in the range of 0.1 to 20 kPa. According to this method, the fermentation productivity of the chemical product can be largely elevated at high stability and a low cost.

摘要翻译： 本发明提供了一种通过连续发酵生产化学产品的方法，包括用分离膜过滤微生物培养物或培养细胞，从滤液中回收产物，同时将非过滤液体保留在培养基中或将其回流至培养物中 将发酵材料添加到培养物中，其中使用平均孔径为0.01μm以上且小于1μm的多孔膜作为分离膜，并且以跨膜压差为0.1〜 20 kPa。 根据该方法，化学产品的发酵生产率可以在高稳定性和低成本的情况下大大提高。