公开(公告)号：US07745671B2

公开(公告)日：2010-06-29

申请号：US10576030

申请日：2004-10-14

申请人： Masanori Yamaguchi ,  Yuichi Kita ,  Tetsuya Mori ,  Kenji Kanbe ,  Akihiro Tomoda ,  Atsushi Takahashi ,  Wakako Ichikawa

发明人： Masanori Yamaguchi ,  Yuichi Kita ,  Tetsuya Mori ,  Kenji Kanbe ,  Akihiro Tomoda ,  Atsushi Takahashi ,  Wakako Ichikawa

IPC分类号： C07C35/16 ,  C07H1/06 ,  C07C35/46

CPC分类号： C12P7/02 ,  C07C29/145 ,  C12N9/0004 ,  C12N9/0006 ,  C12P7/18 ,  C12P7/26

摘要： It is intended to provide a novel NAD+-independent myo-inositol 2-dehydrogenase which converts myo-inositol into scyllo-inosose in the absence of NAD+; a novel enzyme scyllo-inositol dehydrogenase which stereospecifically reduces scyllo-inosose into scyllo-inositol in the presence of NADH or NADPH; and a novel microorganism which belongs to the genus Acetobacter or Burkholderia and can convert myo-inositol into scyllo-inositol. By using these enzymes or the microorganism, scyllo-inositol is produced. Furthermore, scyllo-inositol is purified by adding boric acid and a metal salt to a liquid mixture containing scyllo-inositol and a neutral saccharide other than scyllo-inositol to form a scyllo-inositol/boric acid complex, separating the complex from the liquid mixture, dissolving the thus separated complex in an acid to give an acidic solution or an acidic suspension and then purifying scyllo-inositol from the acidic solution or the acidic suspension.

摘要翻译： 旨在提供一种新的NAD +独立肌醇2-脱氢酶，其在不存在NAD +的情况下将肌醇转化为鲨肌内糖; 在NADH或NADPH的存在下，立体选择性地将鲨肌内糖降解成鲨肌醇的新型鲨肌醇肌醇脱氢酶; 和属于醋杆菌属或伯克霍尔德氏菌属的新型微生物，可将肌醇转化为鲨肌醇。 通过使用这些酶或微生物，产生鲨肌醇。 此外，通过向含有鲨肌醇和除了鲨肌醇之外的中性糖的液体混合物中加入硼酸和金属盐以形成鲨肌醇/硼酸络合物，将所述络合物与液体混合物分离，将鲨肌醇纯化 将如此分离的络合物溶解在酸中，得到酸性溶液或酸性悬浮液，然后从酸性溶液或酸性悬浮液中纯化鲨肌醇。

公开(公告)号：US20130196417A1

公开(公告)日：2013-08-01

申请号：US13791172

申请日：2013-03-08

申请人： Masanori Yamaguchi ,  Yuichi Kita ,  Tetsuya Mori ,  Kenji Kanbe ,  Akihiro Tomoda ,  Atsushi Takahashi ,  Wakako Ichikawa

发明人： Masanori Yamaguchi ,  Yuichi Kita ,  Tetsuya Mori ,  Kenji Kanbe ,  Akihiro Tomoda ,  Atsushi Takahashi ,  Wakako Ichikawa

IPC分类号： C12N9/00

CPC分类号： C12P7/02 ,  C07C29/145 ,  C12N9/0004 ,  C12N9/0006 ,  C12P7/18 ,  C12P7/26

摘要： It is intended to provide a novel NAD+-independent myo-inositol 2-dehydrogenase which converts myo-inositol into scyllo-inosose in the absence of NAD+; a novel enzyme scyllo-inositol dehydrogenase which stereospecifically reduces scyllo-inosose into scyllo-inositol in the presence of NADH or NADPH; and a novel microorganism which belongs to the genus Acetobacter or Burkholderia and can convert myo-inositol into scyllo-inositol. By using these enzymes or the microorganism, scyllo-inositol is produced. Furthermore, scyllo-inositol is purified by adding boric acid and a metal salt to a liquid mixture containing scyllo-inositol and a neutral saccharide other than scyllo-inositol to form a scyllo-inositol/boric acid complex, separating the complex from the liquid mixture, dissolving the thus separated complex in an acid to give an acidic solution or an acidic suspension and then purifying scyllo-inositol from the acidic solution or the acidic suspension.

摘要翻译： 旨在提供一种新的NAD +独立肌醇2-脱氢酶，其在不存在NAD +的情况下将肌醇转化为鲨肌内糖; 在NADH或NADPH的存在下，立体选择性地将鲨肌内糖降解成鲨肌醇的新型鲨肌醇肌醇脱氢酶; 和属于醋杆菌属或伯克霍尔德氏菌属的新型微生物，可将肌醇转化为鲨肌醇。 通过使用这些酶或微生物，产生鲨肌醇。 此外，通过向含有鲨肌醇和除了鲨肌醇之外的中性糖的液体混合物中加入硼酸和金属盐以形成鲨肌醇/硼酸复合物，将络合物与液体混合物分离，将鲨肌醇纯化 将如此分离的络合物溶解在酸中，得到酸性溶液或酸性悬浮液，然后从酸性溶液或酸性悬浮液中纯化鲨肌醇。

公开(公告)号：US08409833B2

公开(公告)日：2013-04-02

申请号：US12712635

申请日：2010-02-25

申请人： Masanori Yamaguchi ,  Yuichi Kita ,  Tetsuya Mori ,  Kenji Kanbe ,  Akihiro Tomoda ,  Atsushi Takahashi ,  Wakako Ichikawa

发明人： Masanori Yamaguchi ,  Yuichi Kita ,  Tetsuya Mori ,  Kenji Kanbe ,  Akihiro Tomoda ,  Atsushi Takahashi ,  Wakako Ichikawa

IPC分类号： C12P19/02

CPC分类号： C12P7/02 ,  C07C29/145 ,  C12N9/0004 ,  C12N9/0006 ,  C12P7/18 ,  C12P7/26

摘要： It is intended to provide a novel NAD+-independent myo-inositol 2-dehydrogenase which converts myo-inositol into scyllo-inosose in the absence of NAD+; a novel enzyme scyllo-inositol dehydrogenase which stereospecifically reduces scyllo-inosose into scyllo-inositol in the presence of NADH or NADPH; and a novel microorganism which belongs to the genus Acetobacter or Burkholderia and can convert myo-inositol into scyllo-inositol. By using these enzymes or the microorganism, scyllo-inositol is produced. Furthermore, scyllo-inositol is purified by adding boric acid and a metal salt to a liquid mixture containing scyllo-inositol and a neutral saccharide other than scyllo-inositol to form a scyllo-inositol/boric acid complex, separating the complex from the liquid mixture, dissolving the thus separated complex in an acid to give an acidic solution or an acidic suspension and then purifying scyllo-inositol from the acidic solution or the acidic suspension.

摘要翻译： 旨在提供一种新的NAD +独立肌醇2-脱氢酶，其在不存在NAD +的情况下将肌醇转化为鲨肌内糖; 在NADH或NADPH的存在下，立体选择性地将鲨肌内糖降解成鲨肌醇的新型鲨肌醇肌醇脱氢酶; 和属于醋杆菌属或伯克霍尔德氏菌属的新型微生物，可将肌醇转化为鲨肌醇。 通过使用这些酶或微生物，产生鲨肌醇。 此外，通过向含有鲨肌醇和除了鲨肌醇之外的中性糖的液体混合物中加入硼酸和金属盐以形成鲨肌醇/硼酸络合物，将络合物与液体混合物分离，将鲨肌醇纯化 将如此分离的络合物溶解在酸中，得到酸性溶液或酸性悬浮液，然后从酸性溶液或酸性悬浮液中纯化鲨肌醇。

公开(公告)号：US20060240534A1

公开(公告)日：2006-10-26

申请号：US10576030

申请日：2004-10-14

申请人： Masanori Yamaguchi ,  Yuichi Kita ,  Tetsuya Mori ,  Kenji Kanbe ,  Akihiro Tomoda ,  Atsushi Takahashi ,  Wakako Ichikawa

发明人： Masanori Yamaguchi ,  Yuichi Kita ,  Tetsuya Mori ,  Kenji Kanbe ,  Akihiro Tomoda ,  Atsushi Takahashi ,  Wakako Ichikawa

IPC分类号： C12P9/00 ,  C12P7/02 ,  C12N9/02 ,  C12N1/21

CPC分类号： C12P7/02 ,  C07C29/145 ,  C12N9/0004 ,  C12N9/0006 ,  C12P7/18 ,  C12P7/26

摘要： It is intended to provide a novel NAD+-independent myo-inositol 2-dehydrogenase which converts myo-inositol into scyllo-inosose in the absence of NAD+; a novel enzyme scyllo-inositol dehydrogenase which stereospecifically reduces scyllo-inosose into scyllo-inositol in the presence of NADH or NADPH; and a novel microorganism which belongs to the genus Acetobacter or Burkholderia and can convert myo-inositol into scyllo-inositol. By using these enzymes or the microorganism, scyllo-inositol is produced. Furthermore, scyllo-inositol is purified by adding boric acid and a metal salt to a liquid mixture containing scyllo-inositol and a neutral saccharide other than scyllo-inositol to form a scyllo-inositol/boric acid complex, separating the complex from the liquid mixture, dissolving the thus separated complex in an acid to give an acidic solution or an acidic suspension and then purifying scyllo-inositol from the acidic solution or the acidic suspension.

摘要翻译： 旨在提供一种新的NAD +独立肌醇2-脱氢酶，其在不存在NAD +的情况下将肌醇转化为鲨肌蛋白; 在NADH或NADPH的存在下，立体选择性地将鲨肌内糖降解成鲨肌醇的新型鲨肌醇肌醇脱氢酶; 和属于醋杆菌属或伯克霍尔德氏菌属的新型微生物，可将肌醇转化为鲨肌醇。 通过使用这些酶或微生物，产生鲨肌醇。 此外，通过向含有鲨肌醇和除了鲨肌醇之外的中性糖的液体混合物中加入硼酸和金属盐以形成鲨肌醇/硼酸络合物，将所述络合物与液体混合物分离，将鲨肌醇纯化 将如此分离的络合物溶解在酸中，得到酸性溶液或酸性悬浮液，然后从酸性溶液或酸性悬浮液中纯化鲨肌醇。

公开(公告)号：US20110059489A1

公开(公告)日：2011-03-10

申请号：US12944592

申请日：2010-11-11

申请人： Tetsuya Mori ,  Wakako Ichikawa ,  Yuichi Kita ,  Yasuyuki Tetsuka

发明人： Tetsuya Mori ,  Wakako Ichikawa ,  Yuichi Kita ,  Yasuyuki Tetsuka

IPC分类号： C12P19/26

CPC分类号： C12R1/885 ,  C12P19/26

摘要： N-Acetyl-D-glucosamine can be produced by cultivating a fungus capable of producing N-acetyl-D-glucosamine, such as Trichoderma hamatum AB 10282 strain (FERM BP-10623) or Trichoderma harzianum AB10283 strain (FERM BP-10624), in a culture medium supplemented with a carbon source other than chitin and chitin oligosaccharide and a nitrogen source to produce and accumulate N-acetyl-D-glucosamine in the culture medium and then collecting N-acetyl-D-glucosamine from the culture medium.

摘要翻译： N-乙酰基-D-葡糖胺可以通过培养能够产生N-乙酰基-D-葡糖胺的真菌，例如木霉木霉AB 10282菌株（FERM BP-10623）或哈茨木霉AB10283菌株（FERM BP-10624）， 在补充有除甲壳素和几丁质寡糖以外的碳源的培养基和氮源中，在培养基中产生并积累N-乙酰基-D-葡糖胺，然后从培养基中收集N-乙酰基-D-葡糖胺。

公开(公告)号：US07998723B2

公开(公告)日：2011-08-16

申请号：US11995104

申请日：2006-07-14

申请人： Tetsuya Mori ,  Wakako Ichikawa ,  Yuichi Kita ,  Yasuyuki Tetsuka

发明人： Tetsuya Mori ,  Wakako Ichikawa ,  Yuichi Kita ,  Yasuyuki Tetsuka

IPC分类号： C12N1/14

CPC分类号： C12R1/885 ,  C12P19/26

摘要： N-Acetyl-D-glucosamine can be produced by cultivating a fungus capable of producing N-acetyl-D-glucosamine, such as Trichoderma hamatum AB10282 strain (FERM BP-10623) or Trichoderma harzianum AB10283 strain (FERM BP-10624), in a culture medium supplemented with a carbon source other than chitin and chitin oligosaccharide and a nitrogen source to produce and accumulate N-acetyl-D-glucosamine in the culture medium and then collecting N-acetyl-D-glucosamine from the culture medium.

摘要翻译： N-乙酰基-D-葡糖胺可以通过培养能够产生N-乙酰基-D-葡糖胺的真菌，如木霉木霉AB10282菌株（FERM BP-10623）或哈茨木霉AB10283菌株（FERM BP-10624），在 补充有几丁质和几丁质寡糖以外的碳源的培养基和氮源，以在培养基中产生并积累N-乙酰基-D-葡糖胺，然后从培养基中收集N-乙酰基-D-葡糖胺。

公开(公告)号：US08383368B2

公开(公告)日：2013-02-26

申请号：US12944592

申请日：2010-11-11

申请人： Tetsuya Mori ,  Wakako Ichikawa ,  Yuichi Kita ,  Yasuyuki Tetsuka

发明人： Tetsuya Mori ,  Wakako Ichikawa ,  Yuichi Kita ,  Yasuyuki Tetsuka

IPC分类号： C12P19/26 ,  C12N1/14

CPC分类号： C12R1/885 ,  C12P19/26

摘要： N-Acetyl-D-glucosamine can be produced by cultivating a fungus capable of producing N-acetyl-D-glucosamine, such as Trichoderma hamatum AB 10282 strain (FERM BP-10623) or Trichoderma harzianum AB10283 strain (FERM BP-10624), in a culture medium supplemented with a carbon source other than chitin and chitin oligosaccharide and a nitrogen source to produce and accumulate N-acetyl-D-glucosamine in the culture medium and then collecting N-acetyl-D-glucosamine from the culture medium.

摘要翻译： N-乙酰基-D-葡糖胺可以通过培养能够产生N-乙酰基-D-葡糖胺的真菌，例如木霉木霉AB 10282菌株（FERM BP-10623）或哈茨木霉AB10283菌株（FERM BP-10624）， 在补充有除甲壳素和几丁质寡糖以外的碳源的培养基和氮源中，在培养基中产生并积累N-乙酰基-D-葡糖胺，然后从培养基中收集N-乙酰基-D-葡糖胺。

公开(公告)号：US20100055746A1

公开(公告)日：2010-03-04

申请号：US11995104

申请日：2006-07-14

申请人： Tetsuya Mori ,  Wakako Ichikawa ,  Yuichi Kita ,  Yasuyuki Tetsuka

发明人： Tetsuya Mori ,  Wakako Ichikawa ,  Yuichi Kita ,  Yasuyuki Tetsuka

IPC分类号： C12P19/02 ,  C12N1/15

CPC分类号： C12R1/885 ,  C12P19/26

摘要： N-Acetyl-D-glucosamine can be produced by cultivating a fungus capable of producing N-acetyl-D-glucosamine, such as Trichoderma hamatum AB10282 strain (FERM BP-10623) or Trichoderma harzianum AB10283 strain (FERM BP-10624), in a culture medium supplemented with a carbon source other than chitin and chitin oligosaccharide and a nitrogen source to produce and accumulate N-acetyl-D-glucosamine in the culture medium and then collecting N-acetyl-D-glucosamine from the culture medium.

摘要翻译： N-乙酰基-D-葡糖胺可以通过培养能够产生N-乙酰基-D-葡糖胺的真菌，如木霉木霉AB10282菌株（FERM BP-10623）或哈茨木霉AB10283菌株（FERM BP-10624），在 补充有几丁质和几丁质寡糖以外的碳源的培养基和氮源，以在培养基中产生并积累N-乙酰基-D-葡糖胺，然后从培养基中收集N-乙酰基-D-葡糖胺。