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公开(公告)号：US06838259B2
公开(公告)日：2005-01-04
申请号：US10168050
申请日：2000-12-14
申请人： Masato Suenaga , Takao Yamada , Osamu Nishimura
发明人： Masato Suenaga , Takao Yamada , Osamu Nishimura
IPC分类号： C07K5/083 , C07K14/47 , C12N1/21 , C12N15/12 , C12N15/62 , C12P21/02 , C12P21/06 , C07K19/00
CPC分类号： C12P21/02 , C07K5/081 , C07K14/4703 , C07K2319/00 , C07K2319/75 , C12N15/62 , C12P21/06
摘要： A KiSS-1 peptide or a salt thereof can be produced in a large scale by subjecting a fusion protein or peptide in which a KiSS-1 peptide is ligated to the N-terminal of a protein or peptide having cysteine at the N-terminal, to the reaction of cleaving the peptide bond on the amino acid side of the cysteine residue.
摘要翻译： 可以通过使连接有KiSS-1肽的融合蛋白或肽与N-半胱氨酸的蛋白质或N-肽的N-末端进行连接而大规模制备KiSS-1肽或其盐， 涉及切割半胱氨酸残基的氨基酸侧上的肽键的反应。
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公开(公告)号：US6066470A
公开(公告)日：2000-05-23
申请号：US872417
申请日：1997-06-10
申请人： Osamu Nishimura , Masato Suenaga , Hiroaki Ohmae , Shinji Tsuji
发明人： Osamu Nishimura , Masato Suenaga , Hiroaki Ohmae , Shinji Tsuji
CPC分类号： C07K1/12
摘要： The present invention provides a method for chemically removing a N-terminal methionine residue selectively, specifically and efficiently from a peptide or a salt thereof having an optionally oxidized methinine residue at its N-terminal. The method reacts a peptide or a salt thereof having an optionally oxidized methinine residue at its N-terminal with an .alpha.-diketone derivative, followed by hydrolysis.
摘要翻译： 本发明提供了一种从其N-末端具有任选氧化的甲硫氨酸残基的肽或其盐选择性地，具体和有效地化学去除N末端甲硫氨酸残基的方法。 该方法使其N-末端具有任选氧化的甲硫氨酸残基的肽或其盐与α-二酮衍生物反应，然后水解。
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公开(公告)号：US06309859B1
公开(公告)日：2001-10-30
申请号：US09436518
申请日：1999-11-09
申请人： Osamu Nishimura , Masato Suenaga , Hiroaki Ohmae , Shinji Tsuji
发明人： Osamu Nishimura , Masato Suenaga , Hiroaki Ohmae , Shinji Tsuji
IPC分类号： C12P2106
CPC分类号： C07K1/12
摘要： The present invention provides a method for chemically removing a N-terminal methionine residue selectively, specifically and efficiently from a peptide or a salt thereof having an optionally oxidized methinine residue at its N-terminal. The method reacts a peptide or a salt thereof having an optionally oxidized methinine residue at its N-terminal with an &agr;-diketone derivative, followed by hydrolysis.
摘要翻译： 本发明提供了一种从其N-末端具有任选氧化的甲硫氨酸残基的肽或其盐选择性地，具体和有效地化学去除N末端甲硫氨酸残基的方法。 该方法使其N-末端具有任选氧化的甲硫氨酸残基的肽或其盐与α-二酮衍生物反应，然后水解。
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公开(公告)号：US06774221B1
公开(公告)日：2004-08-10
申请号：US09806871
申请日：2001-05-07
申请人： Osamu Nishimura , Tsuneo Asano , Masato Suenaga , Hiroaki Ohmae , Norio Okutani
发明人： Osamu Nishimura , Tsuneo Asano , Masato Suenaga , Hiroaki Ohmae , Norio Okutani
IPC分类号： C08H100
CPC分类号： C07K1/12
摘要： A method for removing from a peptide the diketone of the methionine residue, and a method for manufacturing a peptide or salt thereof which does not possess an N-terminal methionine residue, characterized by having a peptide or salt thereof which possesses a diketone of the optionally oxidized N-terminal methionine residue react with 3,4-diaminobenzoic acid or a salt thereof in the presence of acetic acid and sodium formate, formic acid and sodium formate, or formic acid and sodium acetate.
摘要翻译： 从肽中除去甲硫氨酸残基的二酮的方法及其不具有N-末端甲硫氨酸残基的肽或其盐的制造方法，其特征在于具有肽或其盐，其具有任选的 氧化的N-末端甲硫氨酸残基与3,4-二氨基苯甲酸或其盐在乙酸和甲酸钠，甲酸和甲酸钠存在下反应，或甲酸和乙酸钠。
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公开(公告)号：US20110251374A1
公开(公告)日：2011-10-13
申请号：US13140601
申请日：2009-12-18
摘要： Provided is a purification method that purifies an antibody to a high purity, effectively removes antibody polymer (or aggregate), and improves antibody recovery rate. An antibody purification method including a step for treating a solution containing an antibody by mixed mode chromatography in the presence of an amino acid is provided.
摘要翻译： 提供了一种纯化方法，可以将抗体纯化成高纯度，有效去除抗体聚合物（或聚集体），提高抗体回收率。 提供一种抗体纯化方法，其包括在氨基酸存在下通过混合模式色谱法处理含有抗体的溶液的步骤。
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公开(公告)号：US08497358B2
公开(公告)日：2013-07-30
申请号：US13140601
申请日：2009-12-18
摘要： Provided is a purification method that purifies an antibody to a high purity, effectively removes antibody polymer (or aggregate), and improves antibody recovery rate. An antibody purification method including a step for treating a solution containing an antibody by mixed mode chromatography in the presence of an amino acid is provided.
摘要翻译： 提供了一种纯化方法，可以将抗体纯化成高纯度，有效去除抗体聚合物（或聚集体），提高抗体回收率。 提供一种抗体纯化方法，其包括在氨基酸存在下通过混合模式色谱法处理含有抗体的溶液的步骤。
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