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318 条记录 (耗时 0.047 秒)

    Process for producing peptides in E. coli
    1.
    发明授权
    Process for producing peptides in E. coli 失效
    在大肠杆菌中生产肽的方法

    公开(公告)号：US5670340A

    公开(公告)日：1997-09-23

    申请号：US352179

    申请日：1994-12-05

    申请人： Masayuki Yabuta ,  Yuji Suzuki ,  Kazuhiro Ohsuye ,  Takehiro Oshima ,  Seiko Onai ,  Koji Magota ,  Shoji Tanaka

    发明人： Masayuki Yabuta ,  Yuji Suzuki ,  Kazuhiro Ohsuye ,  Takehiro Oshima ,  Seiko Onai ,  Koji Magota ,  Shoji Tanaka

    IPC分类号： C12N15/09 ,  C07K14/58 ,  C07K14/585 ,  C12N9/38 ,  C12N15/16 ,  C12N15/62 ,  C12N15/67 ,  C12N15/70 ,  C12P21/02 ,  C12P21/06 ,  C12R1/19 ,  C12P21/00

    CPC分类号： C07K14/58 ,  C07K14/585 ,  C12N15/62 ,  C12N9/2471 ,  C12Y302/01023 ,  C07K2319/00 ,  C07K2319/23 ,  C07K2319/43 ,  C07K2319/50 ,  C07K2319/75 ,  C07K2319/90

    摘要： The present invention is a process to express a target peptide in a large amount and accumulate the target peptide in host cells in the form of inclusion bodies. The process comprises: A) culturing host cells transformed with a plasmid able to express a gene coding for a fusion protein represented in the formula A--L--B, wherein B is a target peptide, A is a protective peptide comprising a 90-210 amino acid fragment E. coli .beta.-galactosidase, and L is a linker peptide positioned between the C-terminus of the protective peptide and the N-terminus of the target peptide and selected so that when the fusion protein is treated by an enzyme or chemical substance, the target peptide is separated, and wherein the protective peptide and linker peptide are selected so that the isoelectric point of the fusion protein in between 4.9 and 6.9; B) obtaining an insoluble fraction comprising inclusion bodies by homogenization of th cultured transformed cells; C) solubilizing the fusion protein in the inclusion bodies by treatment of the insoluble fraction with a solubilizing agent; and, D) cleaving the peptide bond between the C-terminus of the linker peptide and the N-terminus of the target peptide of the solubilized fusion protein to release the target peptide from the other peptides followed by purification of the target peptide.

    摘要翻译： 本发明是大量表达目标肽并以包涵体的形式在宿主细胞中聚集靶肽的方法。 该方法包括：A）培养用能够表达编码式ALB中表示的融合蛋白的基因的质粒转化的宿主细胞，其中B是靶肽，A是包含90-210个氨基酸片段E的保护肽 大肠杆菌β-半乳糖苷酶，L是位于保护肽的C-末端和靶肽的N末端之间的连接肽，并且被选择使得当融合蛋白被酶或化学物质处理时，靶 分离肽，并且其中选择保护性肽和接头肽，使得融合蛋白的等电点在4.9和6.9之间; B）通过均质培养的转化细胞获得包含包含体的不溶性级分; C）通过用增溶剂处理不溶性级分将融合蛋白溶解在包涵体中; 并且D）切割接头肽的C末端和溶解的融合蛋白的靶肽的N末端之间的肽键，从其他肽释放靶肽，然后纯化目标肽。

    Process for production of secretory Kex2 derivatives
    2.
    发明授权
    Process for production of secretory Kex2 derivatives 失效
    生产分泌型Kex2衍生物的方法

    公开(公告)号：US5885821A

    公开(公告)日：1999-03-23

    申请号：US805918

    申请日：1997-03-04

    申请人： Koji Magota ,  Toyofumi Masuda ,  Yuji Suzuki ,  Masayuki Yabuta

    发明人： Koji Magota ,  Toyofumi Masuda ,  Yuji Suzuki ,  Masayuki Yabuta

    IPC分类号： C12N1/19 ,  C12N9/60 ,  C12N15/57

    CPC分类号： C12N9/60

    摘要： Kex2 protease derivatives obtained by transforming methanol-assimilating with expression vectors containing DNA coding for any amino acid sequence which is an amino acid sequence of Kex2 protease wherein the N-terminus is the Met at position 1 and the C-terminus is one of the amino acids between positions 618 (inclusive) and 698 (inclusive), or a modification of that amino acid sequence, culturing the resulting transformants and recovering the derivatives from the cultures, as well as gene systems coding for the derivatives and a method for producing the Kex2 protease derivatives using the gene systems. Also, a method for excision of desired peptides using the Kex2 protease derivatives.

    摘要翻译： 通过用含有编码任何氨基酸序列的DNA的表达载体转化甲醇同化而获得的Kex2蛋白酶衍生物，所述氨基酸序列是Kex2蛋白酶的氨基酸序列，其中N末端是位于第1位的Met和C末端之一 位置618（含）和698（含）之间的酸，或该氨基酸序列的修饰，培养所得转化体并从培养物中回收衍生物，以及编码衍生物的基因系统和生产Kex2的方法 蛋白酶衍生物使用基因系统。 另外，使用Kex2蛋白酶衍生物切除所需肽的方法。

    Toner container including a cap with two shoulders and image forming device
    9.
    发明授权
    Toner container including a cap with two shoulders and image forming device 有权
    墨粉盒包括一个带有双肩的盖子和成像装置

    公开(公告)号：US09541862B2

    公开(公告)日：2017-01-10

    申请号：US15075810

    申请日：2016-03-21

    申请人： Nobuo Takami ,  Noriyuki Kimura ,  Eisuke Hori ,  Hideki Kimura ,  Kenji Kikuchi ,  Yuji Suzuki

    发明人： Nobuo Takami ,  Noriyuki Kimura ,  Eisuke Hori ,  Hideki Kimura ,  Kenji Kikuchi ,  Yuji Suzuki

    IPC分类号： G03G15/08 ,  G03G21/16

    CPC分类号： G03G15/0879 ,  G03G15/0863 ,  G03G15/0872 ,  G03G15/0886 ,  G03G21/1647 ,  G03G2215/0665 ,  G03G2215/0668 ,  G03G2215/0692 ,  G03G2215/0695 ,  G03G2215/0697

    摘要： A system with a container including a protrusion, and a receiver to receive the container. The receiver includes a slide block that is movable relative to a body of the receiver, the slide block including two surfaces, a first surface, initially contacted by the protrusion of the container during insertion of the container into the receiver, having a first slope to resist movement of the container towards the receiver, and a second surface, subsequently contacted by the protrusion of the container during the insertion of the container into the receiver, having a second slope to interact with the protrusion of the container to assist movement of the container towards the receiver. This construction provides the user with a changing insertion force to indicate proper mounting of the container.

    摘要翻译： 一种具有容器的系统，包括突起，以及用于容纳容器的接收器。 接收器包括可相对于接收器主体移动的滑动块，滑动块包括两个表面，第一表面在容器插入接收器期间最初由容器的突起与容器的突起接触，具有第一斜面 阻止容器朝向接收器的移动，以及在容器插入接收器期间随后与容器的突起接触的第二表面，具有与容器的突起相互作用的第二斜面，以帮助容器的移动 朝向接收器。 这种结构为使用者提供了改变的插入力，以指示容器的适当安装。

    Loropetalum plant named 'Irodori'
    10.
    植物专利
    Loropetalum plant named 'Irodori' 有权
    名为“Irodori”的Loropetalum工厂

    公开(公告)号：US20160262296P1

    公开(公告)日：2016-09-08

    申请号：US14544872

    申请日：2015-02-26

    申请人： Yuji Suzuki

    发明人： Yuji Suzuki

    IPC分类号： A01H5/00

    CPC分类号： A01H5/02

    摘要： A new and distinct Loropetalum cultivar named ‘IRODORI’ is disclosed, characterized by bright pink, white, green and maroon variegated foliage, tall upright and spread habit and bright pink flowers with some flower re-bloom after the initial heavy Spring bloom. The new variety is a Loropetalum, normally produced as an outdoor garden or container plant.

    摘要翻译： 披露了一种名为“IRODORI”的新颖而独特的Loropetalum品种，其特征是明亮的粉红色，白色，绿色和褐红色杂色的叶子，高直立和传播的习惯，鲜亮的粉红色花朵，在初始的春季春季开花后再次绽放。 新品种是Loropetalum，通常生产为户外花园或集装箱厂。