公开(公告)号：US20230235383A1

公开(公告)日：2023-07-27

申请号：US18168385

申请日：2023-02-13

Applicant: Massachusetts Institute of Technology

Inventor： Eyal Perry ,  Jan Tiepelt ,  Joseph Jacobson

IPC: C12Q1/6837 ,  C12Q1/6806 ,  C12Q1/6874

CPC classification number: C12Q1/6837 ,  C12Q1/6806 ,  C12Q1/6874

Abstract: A DNA nanoarray includes a milliscale chip substrate; a microscale binder spot having a uniform surface bound to the substrate; and immobilized oligonucleotide sequences, each linked to the binder. The immobilized oligonucleotide sequences form a monolayer, each having a length that guarantees within a statistical certainty that the immobilized oligonucleotide sequences are each unique. A method of producing the DNA nanoarray includes providing a streptavidin-coated substrate; patterning the substrate by photolithography; and immobilizing biotin-tagged oligonucleotides on the patterned surface. The oligonucleotides each have a unique string of bases. The patterned surface has an array of microscale spots with active streptavidin binding sites. Immobilization includes applying a solution containing the oligonucleotides to the microscale spots; applying a buffer over the patterned surface; and washing the patterned surface in buffered saline solution. Bits and/or spatial patterns may be stored the DNA nanoarray, then read and/or visualized.