公开(公告)号：US20240417769A1

公开(公告)日：2024-12-19

申请号：US18745495

申请日：2024-06-17

Applicant: Massachusetts Institute of Technology

Inventor： Nikhil Lal ,  Eyal Perry ,  Joseph Jacobson

IPC: C12P19/34 ,  B01J19/00

Abstract: A method of polynucleotide synthesis includes a series of enzymatic reactions for restriction, ligation, and amplification using a machine and system that provide efficient droplet-based reactions. The method enables hierarchical assembly with minimal handling in order to address challenges associated with traditional polynucleotide synthesis methods, such as high cost, limited throughput, long synthesis times, and limited ability to synthesize long sequences.

公开(公告)号：US20230235383A1

公开(公告)日：2023-07-27

申请号：US18168385

申请日：2023-02-13

Applicant: Massachusetts Institute of Technology

Inventor： Eyal Perry ,  Jan Tiepelt ,  Joseph Jacobson

IPC: C12Q1/6837 ,  C12Q1/6806 ,  C12Q1/6874

CPC classification number: C12Q1/6837 ,  C12Q1/6806 ,  C12Q1/6874

Abstract: A DNA nanoarray includes a milliscale chip substrate; a microscale binder spot having a uniform surface bound to the substrate; and immobilized oligonucleotide sequences, each linked to the binder. The immobilized oligonucleotide sequences form a monolayer, each having a length that guarantees within a statistical certainty that the immobilized oligonucleotide sequences are each unique. A method of producing the DNA nanoarray includes providing a streptavidin-coated substrate; patterning the substrate by photolithography; and immobilizing biotin-tagged oligonucleotides on the patterned surface. The oligonucleotides each have a unique string of bases. The patterned surface has an array of microscale spots with active streptavidin binding sites. Immobilization includes applying a solution containing the oligonucleotides to the microscale spots; applying a buffer over the patterned surface; and washing the patterned surface in buffered saline solution. Bits and/or spatial patterns may be stored the DNA nanoarray, then read and/or visualized.

公开(公告)号：US20160279630A1

公开(公告)日：2016-09-29

申请号：US15083706

申请日：2016-03-29

Applicant: Massachusetts Institute of Technology

Inventor： Charles Fracchia ,  Matthew Carney ,  Joseph Jacobson ,  Jason Fischman

IPC: B01L3/02 ,  G01N1/28

CPC classification number: B01L3/0217 ,  B01L2200/0684 ,  B01L2200/0689 ,  B01L2200/143 ,  B01L2200/146 ,  B01L2300/023 ,  B01L2300/0627 ,  B01L2300/0829 ,  B01L2300/0832 ,  B01L2300/0864 ,  B01L2400/0487 ,  B01L2400/0622

Abstract: A pipetting system identifies and records data regarding pipetting events, including the time and well into which a pipette dispenses liquid and the volume of liquid dispensed into the well. The pipetting system also measures and records other data regarding the context of pipetting events. This other data includes the speed of pipetting, the pressure exerted on a plunger of the pipette by a finger of a user, and the temperature, humidity and lighting conditions at a well tray into which the liquid is dispensed. The pipetting system includes a pipette that has multiple cylinders and multiple corresponding pistons. Each cylinder has different diameter. Thus, a down-stroke of the pistons causes each of the cylinders to expel a different amount of air. A rotatable mechanism selects which cylinder is connected by an airway to a pipette tip and which other cylinders vent directly to the ambient atmosphere.

Abstract translation: 移液系统识别和记录关于移液事件的数据，包括移液管分配液体的时间和质量以及分配到孔中的液体体积。 移液系统还测量和记录关于移液事件背景的其他数据。 其他数据包括移液速度，使用者的手指施加在移液管的柱塞上的压力以及在分配液体的井盘中的温度，湿度和照明条件。 移液系统包括具有多个气缸和多个相应活塞的移液管。 每个气缸的直径不同。 因此，活塞的下冲程导致每个气缸排出不同量的空气。 可旋转机构选择哪个气缸通过气道连接到移液管尖端，哪个气缸直接通向环境大气。