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    Methods and systems for identification of macromolecules
    9.
    发明申请
    Methods and systems for identification of macromolecules 审中-公开
    用于鉴定大分子的方法和系统

    公开(公告)号：US20050192755A1

    公开(公告)日：2005-09-01

    申请号：US10789424

    申请日：2004-02-27

    申请人： Srinivasa Nagalla ,  Brian Searle ,  Surendra Dasari ,  Mark Turner

    发明人： Srinivasa Nagalla ,  Brian Searle ,  Surendra Dasari ,  Mark Turner

    IPC分类号： G01N33/48 ,  G01N33/50 ,  G01N33/53 ,  G06F19/00

    CPC分类号： H01J49/0036 ,  G16B30/00 ,  G16B50/00

    摘要： A method is provided for identifying sequences of molecules and sequence modifications from mass spectrometry data. At least one de novo sequence is produced from mass spectrometry data of sequences of molecules,. At least one mass-based alignment is calculated between each de novo sequence and sequences in a sequence database. The molecular masses of molecules in the de novo sequence are compared to molecular masses of molecules in each sequence in the sequence database. Mass differences of modification sites are interpreted between the sequence in the sequence database and the de novo sequence that have been identified by the mass-based alignment as modifications identified in a modification catalog. At least one match score for the mass-based alignment is calculated that provides an indication of matching between the sequence in the sequence database and the de novo sequence. Sequences in the sequence database are identified from mass-based alignments in response to the match scores. Identifications of sequences in the sequence database are grouped from at least one de novo sequence into an identified macromolecule list that agrees with the de novo sequencing results.

    摘要翻译： 提供了一种用于从质谱数据鉴定分子序列和序列修饰的方法。 从分子序列的质谱数据产生至少一个从头序列。 在序列数据库中的每个从头序列和序列之间计算至少一个基于质量的比对。 将从头序列中的分子的分子质量与序列数据库中的每个序列中的分子的分子量进行比较。 修饰位点的质量差异在序列数据库中的序列和通过基于质量的校准被鉴定的从头序列中作为在修改目录中确定的修饰来解释。 计算用于基于质量的比对的至少一个匹配得分，其提供序列数据库中的序列与从头序列之间的匹配的指示。 序列数据库中的序列根据匹配分数从基于质量的比对中确定。 序列数据库中序列的识别从至少一个从头序列分组到与从头测序结果一致的鉴定的大分子列表中。