公开(公告)号：US20220317123A1

公开(公告)日：2022-10-06

申请号：US17705000

申请日：2022-03-25

Applicant: McMaster University

Inventor： Yingfu Li ,  Meghan Rothenbroker ,  Erin M. McConnell ,  Carlos D. M. Filipe ,  Jimmy Gu

IPC: G01N33/569 ,  C12N15/113 ,  G01N33/542

Abstract: This disclosure relates to DNAzymes and biosensors for detecting pathogenic bacteria, and in particular, for detecting Legionella pneumophila. This disclosure also provides a method for detecting the presence of Legionella pneumophila in a test sample, comprising: a) contacting said test sample with the DNAzyme or biosensor described herein, wherein the DNAzyme comprises a detectable label; b) allowing cleavage of the DNAzyme if a target is present, thereby releasing the detectable label; and c) measuring a detectable signal if the portion of the DNAzyme comprising the detectable label is released, wherein the RNA cleavage activity of the DNAzyme is activated by a target from Legionella pneumophila.

公开(公告)号：US20210262013A1

公开(公告)日：2021-08-26

申请号：US17180337

申请日：2021-02-19

Applicant: McMaster University

Inventor： Carlos Filipe ,  Yingfu Li ,  Sahar Esmaeili Samani ,  Dingran Chang ,  Erin M. McConnell ,  Meghan Rothenbroker

IPC: C12Q1/6823

Abstract: This disclosure relates to biosensors, and in particular, biosensors based on nucleic acid cleaving enzymes such as ribonucleotide-cleaving DNAzymes for the detection of analytes, and methods of use.

公开(公告)号：US11692215B2

公开(公告)日：2023-07-04

申请号：US17180337

申请日：2021-02-19

Applicant: McMaster University

Inventor： Carlos Filipe ,  Yingfu Li ,  Sahar Esmaeili Samani ,  Dingran Chang ,  Erin M. McConnell ,  Meghan Rothenbroker

IPC: C12Q1/6825 ,  C12Q1/6823

CPC classification number: C12Q1/6823 ,  C12Q1/6825 ,  C12Q1/6825 ,  C12Q2521/301 ,  C12Q2521/327 ,  C12Q2521/337 ,  C12Q2521/345 ,  C12Q2527/127 ,  C12Q2563/107

Abstract: This disclosure relates to biosensors, and in particular, biosensors based on nucleic acid cleaving enzymes such as ribonucleotide-cleaving DNAzymes for the detection of analytes, and methods of use.